Name: Mesorhizobium robiniae DSM 100022
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130527

Type strain:

Species: Mesorhizobium robiniae

Culture col. no.: DSM 100022, ACCC 14543, HAMBI 3082, CCNWYC 115

Strain history: <- HAMBI <- G. H. Wei, College of Life Sciences, Shaanxi, China; CCNWYC 115

NCBI tax ID(s): 559315 (species)

SI-ID 402089

DSM 100022

Section

Mesorhizobium robiniae DSM 100022 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodule of Robinia pseudoacacia.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Phyllobacteriaceae*
	Genus	*Mesorhizobium*
	Species	**Mesorhizobium robiniae**
	Full Scientific Name (LPSN)	Mesorhizobium robiniae Zhou et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29713]	Gram stain	negative

[Ref.: #29713]	Cell length	1-2.5 µm

[Ref.: #29713]	Cell width	0.3-0.6 µm

[Ref.: #29713]	Cell shape	rod-shaped

[Ref.: #29713]	Motility	yes

[Ref.: #22035]

Incubation period

2-3 days

[Ref.: #29713]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22035]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #22035]

Culture medium growth

[Ref.: #22035]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22035]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #22035]	growth	30 °C
[Ref.: #29713]	growth	15-40 °C
[Ref.: #29713]	optimum	25-30 °C

[Ref.: #22035]	Temperature range	mesophilic
[Ref.: #29713]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29713]			optimum	06-08
[Ref.: #29713]			growth	06-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29713]

Oxygen tolerance

aerobe

[Ref.: #29713]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29713]		NaCl		growth	5	%
[Ref.: #29713]		NaCl		optimum	5	%

[Ref.: #29713]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29713]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #29713]	fructose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29713]	glucose ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #29713]	lactose ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29713]	maltose ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #29713]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #29713]	raffinose ChEBI	+	carbon source
[Ref.: #29713]	ribose ChEBI	+	carbon source
[Ref.: #29713]	sorbitol ChEBI	+	carbon source
[Ref.: #29713]	succinate ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #29713]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #22035]	catalase	+	1.11.1.6
[Ref.: #22035]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #22035]	24145	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22035]	Sample type/isolated from	root nodule of Robinia pseudoacacia
[Ref.: #22035]	Host species	Robinia pseudoacacia
[Ref.: #22035]	Geographic location (country and/or sea, region)	Yangling, Shaanxi Province
[Ref.: #22035]	Country	China
[Ref.: #22035]	Country ISO 3 Code	CHN
[Ref.: #22035]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU849582 (>99% sequence identity) for Mesorhizobium from Microbeatlas

Aquatic Samples	507
Soil Samples	2635
Animal Samples	282
Plant Samples	1035
Total Samples	4459

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22035]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22035]	Mesorhizobium robiniae strain CCNWYC 115 16S ribosomal RNA gene, partial sequence	EU849582	1356	ENA	559315 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mesorhizobium robiniae DSM 100022	2928416989	draft	JGI IMG/M	559315 tax ID	*

[Ref.: #29713]

GC-content

61.5 mol%

Availability in culture collections External links

[Ref.: #22035]

Culture collection no.

DSM 100022, ACCC 14543, HAMBI 3082, CCNWYC 115

[Ref.: #89050]

SI-ID 402089

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mesorhizobium robiniae sp. nov., isolated from root nodules of Robinia pseudoacacia.	Zhou PF, Chen WM, Wei GH	Int J Syst Evol Microbiol	10.1099/ijs.0.019356-0	2009	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22035

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100022

#29713

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26096 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#89050

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402089.1 )

#26096

IJSEM 2552 2010 ( DOI 10.1099/ijs.0.019356-0 , PubMed 20008111 )

* These data were automatically processed and therefore are not curated

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