Strain identifier

BacDive ID: 130507

Type strain: Yes

Species: Glaciimonas singularis

Strain Designation: A2-57

Strain history: CIP <- 2012, P.V. Morais, Coimbra Univ., IMAR-CMA, Coimbra, Portugal: strain A2-57

NCBI tax ID(s): 1229971 (species)

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General

@ref: 22084

BacDive-ID: 130507

DSM-Number: 100199

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Glaciimonas singularis A2-57 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from acidic water collected from a water treatment plant in former uranium mine.

NCBI tax id

  • NCBI tax id: 1229971
  • Matching level: species

strain history

@refhistory
22084<- CIP <- A. P. Chung, Univ. Coimbra, Portugal; A2-57
67772Chung A.P. University of Coimbra
122633CIP <- 2012, P.V. Morais, Coimbra Univ., IMAR-CMA, Coimbra, Portugal: strain A2-57

doi: 10.13145/bacdive130507.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Oxalobacteraceae
  • genus: Glaciimonas
  • species: Glaciimonas singularis
  • full scientific name: Glaciimonas singularis Chung et al. 2013

@ref: 22084

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Glaciimonas

species: Glaciimonas singularis

full scientific name: Glaciimonas singularis Chung et al. 2013

strain designation: A2-57

type strain: yes

Morphology

cell morphology

  • @ref: 30878
  • gram stain: negative
  • cell length: 1.5 µm
  • cell width: 0.9 µm
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 30878
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39786MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
22084REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)yesName: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled waterhttps://mediadive.dsmz.de/medium/830c
122633CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
22084positivegrowth25mesophilic
30878positivegrowth10-30
30878positiveoptimum25mesophilic
39786positivegrowth25mesophilic
67772positiveminimum10psychrophilic
67772positiveoptimum25mesophilic
67772positivemaximum30mesophilic

culture pH

@refabilitytypepHPH range
30878positivegrowth5.0-10.0alkaliphile
30878positiveoptimum6.5
67772positiveoptimum6.0-7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 30878
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30878
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3087816449alanine+carbon source
3087822599arabinose+carbon source
3087822653asparagine+carbon source
3087835391aspartate+carbon source
3087828757fructose+carbon source
3087828260galactose+carbon source
3087817234glucose+carbon source
3087829987glutamate+carbon source
3087817754glycerol+carbon source
3087827570histidine+carbon source
30878182404-hydroxy-L-proline+carbon source
3087824996lactate+carbon source
3087815792malonate+carbon source
3087829864mannitol+carbon source
3087837684mannose+carbon source
3087817268myo-inositol+carbon source
30878506227N-acetylglucosamine+carbon source
3087826271proline+carbon source
3087817272propionate+carbon source
3087851850methyl pyruvate+carbon source
3087826490quinate+carbon source
3087830911sorbitol+carbon source
3087853423tween 40+carbon source
3087853426tween 80+carbon source

enzymes

@refvalueactivityec
30878acid phosphatase+3.1.3.2
30878alkaline phosphatase+3.1.3.1
30878catalase+1.11.1.6
30878cytochrome oxidase+1.9.3.1
30878urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
22084acidic water collected from a water treatment plant in former uranium minePoco das Cobras, Urgeirica, Central PortugalPortugalPRTEurope
67772Uranium mine wastewater treatment plant (acidic water sample -pH 5)Viseu, UrgeiriçaPortugalPRTEurope2009
122633Environment, Soil, waste water treatment plantUrgeiricaPortugalPRTEurope2009

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Engineered#Waste#Water treatment plant
#Environmental#Aquatic
#Condition#Acidic

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
220841Risk group (German classification)
677721
1226331Risk group (French classification)

Sequence information

16S sequences

  • @ref: 22084
  • description: Glaciimonas singularis strain A2-57 16S ribosomal RNA gene, partial sequence
  • accession: JX218021
  • length: 1507
  • database: ena
  • NCBI tax ID: 1229971

GC content

  • @ref: 30878
  • GC-content: 55

External links

@ref: 22084

culture collection no.: DSM 100199, CIP 110539, LMG 27070, UCCCB36

straininfo link

  • @ref: 89030
  • straininfo: 380616

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23178726Glaciimonas singularis sp. nov., isolated from a uranium mine wastewater treatment plant.Chung AP, Tiago I, Nobre MF, Verissimo A, Morais PVInt J Syst Evol Microbiol10.1099/ijs.0.046581-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, *Mining, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, *Phylogeny, Portugal, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis, Uranium, Waste Water/*microbiology, Water MicrobiologyGenetics
Phylogeny24478210Noviherbaspirillum suwonense sp. nov., isolated from an air sample.Kim SJ, Moon JY, Weon HY, Hong SB, Seok SJ, Kwon SWInt J Syst Evol Microbiol10.1099/ijs.0.057372-02014*Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Ubiquinone/chemistryGenetics
Phylogeny26184665Glaciimonas alpina sp. nov. isolated from alpine glaciers and reclassification of Glaciimonas immobilis Cr9-12 as the type strain of Glaciimonas alpina sp. nov.Frasson D, Udovicic M, Frey B, Lapanje A, Zhang DC, Margesin R, Sievers MInt J Syst Evol Microbiol10.1099/ijs.0.0001742015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Ice Cover/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Switzerland, Ubiquinone/chemistryGenetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22084Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100199Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100199)
30878Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172720828776041
39786Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8409
67772Curators of the UCCCBhttps://ucccb.uc.pt/
89030Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID380616.1
122633Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110539Collection of Institut Pasteur (CIP 110539)