Strain identifier

BacDive ID: 130449

Type strain: Yes

Species: Acinetobacter boissieri

Strain Designation: SAP284.1

Strain history: CIP <- 2012, CECT <- Alvarez-Perez strain: SAP284.1

NCBI tax ID(s): 1219383 (species)

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General

@ref: 21450

BacDive-ID: 130449

DSM-Number: 29976

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, ovoid-shaped

description: Acinetobacter boissieri SAP284.1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from floral nectar of wild Mediterranean insect-pollinated plants,Muscari comosum.

NCBI tax id

  • NCBI tax id: 1219383
  • Matching level: species

strain history

@refhistory
21450<- CECT <- S. Álvarez-Pérez, CSIC, Estación Biológica de Doñana
116833CIP <- 2012, CECT <- Alvarez-Perez strain: SAP284.1

doi: 10.13145/bacdive130449.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter boissieri
  • full scientific name: Acinetobacter boissieri Álvarez-Pérez et al. 2013

@ref: 21450

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter boissieri

full scientific name: Acinetobacter boissieri Álvarez-Pérez et al. 2013

strain designation: SAP284.1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30741negative1.5 µm1.5 µmovoid-shapedno
116833negativerod-shapedno

colony morphology

@refincubation period
214502-3 days
634862 days

pigmentation

  • @ref: 30741
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
32731MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
21450TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
116833CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
21450positivegrowth30mesophilic
30741positivegrowth04-30
32731positivegrowth30mesophilic
63486positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30741aerobe
63486aerobe

spore formation

  • @ref: 30741
  • spore formation: no

observation

  • @ref: 30741
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3074122653asparagine+carbon source
3074135391aspartate+carbon source
3074128757fructose+carbon source
3074124265gluconate+carbon source
3074129987glutamate+carbon source
3074125115malate+carbon source
3074137684mannose+carbon source
3074126271proline+carbon source
3074130031succinate+carbon source
3074117992sucrose+carbon source
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
21450catalase+1.11.1.6
21450cytochrome-c oxidase-1.9.3.1
30741catalase+1.11.1.6
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
21450--+-----------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling date
21450floral nectar of wild Mediterranean insect-pollinated plants,Muscari comosumMuscari comosumHinojos (Huelva)SpainESPEurope
63486Floral nectar,plant species Muscari comosumHinojos,(Huelva)SpainESPEurope2011
116833Plant, Floral nectar of wild Mediterranean insect-pollinated plants

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Flower
#Host Body Product#Plant#Nectar

taxonmaps

  • @ref: 69479
  • File name: preview.99_92285.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_695;97_810;98_63520;99_92285&stattab=map
  • Last taxonomy: Acinetobacter boissieri subclade
  • 16S sequence: JQ771141
  • Sequence Identity:
  • Total samples: 1385
  • soil counts: 82
  • aquatic counts: 171
  • animal counts: 972
  • plant counts: 160

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
214501Risk group (German classification)
1168331Risk group (French classification)

Sequence information

16S sequences

  • @ref: 21450
  • description: Acinetobacter boissieri strain SAP 284.1 16S ribosomal RNA gene, partial sequence
  • accession: JQ771141
  • length: 1498
  • database: ena
  • NCBI tax ID: 1219383

GC content

  • @ref: 21450
  • GC-content: 37.8

External links

@ref: 21450

culture collection no.: DSM 29976, CECT 8128, LMG 26959, CCUG 68432, CIP 110548, SAP 284.1

straininfo link

  • @ref: 88976
  • straininfo: 379901

literature

  • topic: Phylogeny
  • Pubmed-ID: 22904213
  • title: Acinetobacter nectaris sp. nov. and Acinetobacter boissieri sp. nov., isolated from floral nectar of wild Mediterranean insect-pollinated plants.
  • authors: Alvarez-Perez S, Lievens B, Jacquemyn H, Herrera CM
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.043489-0
  • year: 2012
  • mesh: Acinetobacter/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Flowers/microbiology, Insecta, Mediterranean Region, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Plant Nectar/*analysis, Pollination, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21450Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29976Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29976)
30741Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172707228776041
32731Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8419
63486Curators of the CCUGhttps://www.ccug.se/strain?id=68432Culture Collection University of Gothenburg (CCUG) (CCUG 68432)
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88976Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID379901.1
116833Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110548Collection of Institut Pasteur (CIP 110548)