General

@ref: 21661

BacDive-ID: 130402

DSM-Number: 29295

keywords: 16S sequence, Bacteria, aerobe, mesophilic

description: Methylopila oligotropha 2395A is an aerobe, mesophilic bacterium that was isolated from soil from territory of a salt mine.

NCBI tax id

• NCBI tax id: 1282438
• Matching level: species

strain history

• @ref: 21661
• history: <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; 2395A

doi: 10.13145/bacdive130402.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/pseudomonadota
• domain: Bacteria
• phylum: Pseudomonadota
• class: Alphaproteobacteria
• order: Hyphomicrobiales
• family: Methylocystaceae
• genus: Methylopila
• species: Methylopila oligotropha
• full scientific name: Methylopila oligotropha Poroshina et al. 2014

@ref: 21661

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylocystaceae

genus: Methylopila

species: Methylopila oligotropha

full scientific name: Methylopila oligotropha Poroshina et al. 2014

strain designation: 2395A

type strain: yes

Morphology

colony morphology

• @ref: 63029
• incubation period: 3 days

multimedia

• @ref: 21661
• multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_29295.jpg
• intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

• @ref: 21661
• name: NUTRIENT AGAR (DSMZ Medium 1)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1
• composition: Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Agar 15.0 g/l NaCl 5.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Methanol Distilled water

culture temp

Physiology and metabolism

oxygen tolerance

• @ref: 63029
• oxygen tolerance: aerobe

metabolite utilization

metabolite production

• @ref: 68369
• Chebi-ID: 35581
• metabolite: indole
• production: no

metabolite tests

• @ref: 68369
• Chebi-ID: 35581
• metabolite: indole
• indole test: -

enzymes

API 20NE

Isolation, sampling and environmental information

isolation

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_1985.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_1101;97_1289;98_1553;99_1985&stattab=map
• Last taxonomy: Methylopila
• 16S sequence: KC243676
• Sequence Identity:
• Total samples: 274
• soil counts: 79
• aquatic counts: 89
• animal counts: 81
• plant counts: 25

Safety information

risk assessment

• @ref: 21661
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 21661
• description: Methylopila oligotropha strain 2395A from Russia 16S ribosomal RNA gene, partial sequence
• accession: KC243676
• length: 1447
• database: ena
• NCBI tax ID: 1282438

External links

@ref: 21661

culture collection no.: DSM 29295, CCUG 63805, VKM B-2788

straininfo link

• @ref: 88934
• straininfo: 400437

literature

• topic: Phylogeny
• Pubmed-ID: 30972598
• title: Methylopila carotae sp. nov., a facultative methylotroph, isolated from a root of Daucus carota L.
• authors: Kaparullina EN, Chemodurova AA, Agafonova NV, Karataeva TA, Detkova EN, Trotsenko YA, Doronina NV
• journal: Antonie Van Leeuwenhoek
• DOI: 10.1007/s10482-019-01263-x
• year: 2019
• mesh: Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Daucus carota/*microbiology, Fatty Acids/analysis, Methylocystaceae/*classification/genetics/*isolation & purification/physiology, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Plant Roots/microbiology, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
• topic2: Transcriptome

Reference