Name: Methylopila oligotropha 2395A
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130402

Type strain:

Species: Methylopila oligotropha

Strain Designation: 2395A

Culture col. no.: DSM 29295, CCUG 63805, VKM B-2788

Strain history: <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; 2395A

NCBI tax ID(s): 1282438 (species)

SI-ID 400437

DSM 29295, CCUG 63805

Section

Methylopila oligotropha 2395A is an aerobe, mesophilic bacterium that was isolated from soil from territory of a salt mine.

aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylocystaceae*
	Genus	*Methylopila*
	Species	**Methylopila oligotropha**
	Full Scientific Name (LPSN)	Methylopila oligotropha Poroshina et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #63029]

Incubation period

3 days

Multimedia content

[Ref.: #21661]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21661]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #21661]

Culture medium growth

[Ref.: #21661]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21661]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #21661]			growth	28 °C
[Ref.: #63029]			growth	30 °C

[Ref.: #21661]	Temperature range	mesophilic
[Ref.: #63029]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63029]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21661]	23290	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21661]	Sample type/isolated from	soil from territory of a salt mine
[Ref.: #21661]	Geographic location (country and/or sea, region)	Perm region, Solikamsk
[Ref.: #21661]	Country	Russia
[Ref.: #21661]	Country ISO 3 Code	RUS
[Ref.: #21661]	Continent	Europe

[Ref.: #63029]	Sample type/isolated from	Sludge from saline source
[Ref.: #63029]	Sampling date	2009
[Ref.: #63029]	Geographic location (country and/or sea, region)	Perm region,Solikamsk
[Ref.: #63029]	Country	Russia
[Ref.: #63029]	Country ISO 3 Code	RUS
[Ref.: #63029]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KC243676 (>99% sequence identity) for Methylopila from Microbeatlas

Aquatic Samples	89
Soil Samples	79
Animal Samples	81
Plant Samples	25
Total Samples	274

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21661]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21661]	Methylopila oligotropha strain 2395A from Russia 16S ribosomal RNA gene, partial sequence	KC243676	1447	ENA	1282438 tax ID

Availability in culture collections External links

[Ref.: #21661]

Culture collection no.

DSM 29295, CCUG 63805, VKM B-2788

[Ref.: #88934]

SI-ID 400437

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylopila carotae sp. nov., a facultative methylotroph, isolated from a root of Daucus carota L.	Kaparullina EN, Chemodurova AA, Agafonova NV, Karataeva TA, Detkova EN, Trotsenko YA, Doronina NV	Antonie Van Leeuwenhoek	10.1007/s10482-019-01263-x	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21661

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29295

#63029 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63805

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88934

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400437.1 )

* These data were automatically processed and therefore are not curated

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