Strain identifier

BacDive ID: 130361

Type strain: No

Species: Murimonas intestini

Strain Designation: SRB-509-4-S-H

Strain history: <- T. Clavel, Research Center for Nutrition and Food Sci. (ZIEL), TU Munich, Germany <- K. Kläring

NCBI tax ID(s): 1337051 (species)

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General

@ref: 22148

BacDive-ID: 130361

DSM-Number: 27577

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, animal pathogen

description: Murimonas intestini SRB-509-4-S-H is an anaerobe, mesophilic animal pathogen that was isolated from caecal content of a 17-week-old female heterozygous TNF DeltaARE/+ mouse that was fed a standard experimental diet supplemented with ground flaxseeds.

NCBI tax id

  • NCBI tax id: 1337051
  • Matching level: species

strain history

  • @ref: 22148
  • history: <- T. Clavel, Research Center for Nutrition and Food Sci. (ZIEL), TU Munich, Germany <- K. Kläring

doi: 10.13145/bacdive130361.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Murimonas
  • species: Murimonas intestini
  • full scientific name: Murimonas intestini Kläring et al. 2015

@ref: 22148

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Murimonas

species: Murimonas intestini

full scientific name: Murimonas intestini Kläring et al. 2015

strain designation: SRB-509-4-S-H

type strain: no

Morphology

colony morphology

  • @ref: 63142
  • incubation period: 2 days

Culture and growth conditions

culture medium

  • @ref: 22148
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
63142positivegrowth37mesophilic
22148positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22148anaerobe
63142anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22148--++-+/-++---+-+---------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
22148caecal content of a 17-week-old female heterozygous TNF DeltaARE/+ mouse that was fed a standard experimental diet supplemented with ground flaxseedsFreising-WeihenstephanGermanyDEUEurope
63142Mouse intestineWeihenstephan,FreisingGermanyDEUEurope2012-08-01

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Gastrointestinal tract#Large intestine
#Host Body Product#Gastrointestinal tract#Caecal content

taxonmaps

  • @ref: 69479
  • File name: preview.99_63911.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_22070;97_27562;98_45214;99_63911&stattab=map
  • Last taxonomy: Murimonas intestini subclade
  • 16S sequence: KF601349
  • Sequence Identity:
  • Total samples: 10246
  • soil counts: 24
  • aquatic counts: 74
  • animal counts: 10124
  • plant counts: 24

Safety information

risk assessment

  • @ref: 22148
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22148
  • description: Murimonas intestini strain SRB-509-4-S-H 16S ribosomal RNA gene, partial sequence
  • accession: KF601349
  • length: 1357
  • database: ena
  • NCBI tax ID: 1337051

Genome sequences

  • @ref: 66792
  • description: Murimonas intestini DSM 27577
  • accession: GCA_024622165
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1337051

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno91.389no
gram-positiveyes90.542no
anaerobicyes98.433yes
aerobicno98.185yes
halophileno80.46no
spore-formingyes78.963no
motileno79.429no
glucose-fermentyes70.551no
thermophileno99.253no
glucose-utilyes91.556no

External links

@ref: 22148

culture collection no.: DSM 27577, CCUG 64595

straininfo link

  • @ref: 88893
  • straininfo: 399488

literature

  • topic: Phylogeny
  • Pubmed-ID: 25519299
  • title: Murimonas intestini gen. nov., sp. nov., an acetate-producing bacterium of the family Lachnospiraceae isolated from the mouse gut.
  • authors: Klaring K, Just S, Lagkouvardos I, Hanske L, Haller D, Blaut M, Wenning M, Clavel T
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.000030
  • year: 2014
  • mesh: Animals, Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Female, Gram-Positive Bacteria/*classification/genetics/isolation & purification, Humans, Intestines/*microbiology, Mice/microbiology, Mice, Inbred C57BL, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22148Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27577Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27577)
63142Curators of the CCUGhttps://www.ccug.se/strain?id=64595Culture Collection University of Gothenburg (CCUG) (CCUG 64595)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88893Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399488.1