Strain identifier
BacDive ID: 130245
Type strain: 
Species: Clostridium frigoriphilum
Strain Designation: 14F
Strain history: <- S. Pecheritsyna, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; 14F <- S. A. Pecheritsyna {2005}
NCBI tax ID(s): 2843317 (species)
version 9.1 (current version)
General
@ref: 21879
BacDive-ID: 130245
DSM-Number: 17811
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming
description: Clostridium frigoriphilum 14F is an anaerobe, spore-forming bacterium that was isolated from water brine within permafrost.
NCBI tax id
- NCBI tax id: 2843317
- Matching level: species
strain history
- @ref: 21879
- history: <- S. Pecheritsyna, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; 14F <- S. A. Pecheritsyna {2005}
doi: 10.13145/bacdive130245.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium frigoriphilum
- full scientific name: Clostridium frigoriphilum Dorn-In et al. 2018
@ref: 21879
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium sp.
full scientific name: Clostridium sp.
strain designation: 14F
type strain: no
Morphology
colony morphology
- @ref: 21879
- incubation period: 8-14 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 21879 | WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a) | yes | https://mediadive.dsmz.de/medium/339a | Name: WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l D-Glucose 5.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water |
| 21879 | PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104c.pdf |
culture temp
- @ref: 21879
- growth: positive
- type: growth
- temperature: 5
Physiology and metabolism
oxygen tolerance
- @ref: 21879
- oxygen tolerance: anaerobe
spore formation
- @ref: 69481
- spore formation: yes
- confidence: 100
Isolation, sampling and environmental information
isolation
- @ref: 21879
- sample type: water brine within permafrost
- geographic location: Northern Eurasia, Siberia, Kolyma Lowland
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | |
| #Environmental | #Terrestrial | #Permafrost |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_505.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_327;97_364;98_413;99_505&stattab=map
- Last taxonomy: Clostridium
- 16S sequence: EF570920
- Sequence Identity:
- Total samples: 18467
- soil counts: 9246
- aquatic counts: 5428
- animal counts: 1548
- plant counts: 2245
Sequence information
16S sequences
- @ref: 21879
- description: Clostridium frigoriphilum strain 14F 16S ribosomal RNA gene, partial sequence
- accession: EF570920
- length: 1388
- database: nuccore
- NCBI tax ID: 443253
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium sp. DSM 17811 | GCA_018861905 | contig | ncbi | 2843317 |
| 66792 | Clostridium sp. DSM 17811 | 2843317.3 | wgs | patric | 2843317 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 100 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 68.91 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 84.76 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 78.274 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 94.086 | yes |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 91.911 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 79.92 | no |
External links
@ref: 21879
culture collection no.: DSM 17811, VKM B-2368
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21879 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17811 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17811) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |