Strain identifier

BacDive ID: 13003

Type strain: No

Species: Pseudomonas stutzeri

Strain Designation: AW-1

Strain history: CIP <- 2003, A. Wolterink, Wageningen Univ., Wageningen, The Netherlands, Pseudomonas chloritidismutans: strain AW-1

NCBI tax ID(s): 203192 (species), 316 (species)

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General

@ref: 5100

BacDive-ID: 13003

DSM-Number: 13592

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Pseudomonas stutzeri AW-1 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from airlift reactor.

NCBI tax id

NCBI tax idMatching level
203192species
316species

strain history

@refhistory
5100<- A. Wolterink, Univ. Wageningen; AW-1
67770IAM 15183 <-- KCTC 12116 <-- DSM 13592 <-- A. Wolterink AW-1.
123337CIP <- 2003, A. Wolterink, Wageningen Univ., Wageningen, The Netherlands, Pseudomonas chloritidismutans: strain AW-1

doi: 10.13145/bacdive13003.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 5100

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: AW-1

type strain: no

Morphology

cell morphology

  • @ref: 123337
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 123337
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5100PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM (DSMZ Medium 944)yeshttps://mediadive.dsmz.de/medium/944Name: PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM (DSMZ Medium 944) Composition: NaHCO3 3.73 g/l Na-acetate x 3 H2O 2.72 g/l NaClO3 1.06 g/l Na2HPO4 0.53 g/l Na2S x 9 H2O 0.5 g/l NH4HCO3 0.44 g/l KH2PO4 0.41 g/l CaCl2 0.11 g/l MgCl2 0.1 g/l NaOH 0.005 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l alpha-lipoic acid 5e-05 g/l Riboflavin 5e-05 g/l Pantothenic acid 5e-05 g/l Vitamin B12 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Na2WO4 x 2 H2O 3e-05 g/l Na2SeO3 x 5 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l Nicotinic acid 2.5e-05 g/l Nicotine amide 2.5e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Pyridoxamine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
5100R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
34277MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123337CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5100positivegrowth30mesophilic
34277positivegrowth30mesophilic
59220positivegrowth30mesophilic
67770positivegrowth30mesophilic
123337positivegrowth25-37mesophilic
123337nogrowth5psychrophilic
123337nogrowth10psychrophilic
123337nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5100aerobe
5100anaerobe
59220aerobe
123337obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
123337NaClpositivegrowth0-6 %
123337NaClnogrowth8 %
123337NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12333716947citrate-carbon source
1233374853esculin-hydrolysis
12333717632nitrate+reduction
12333716301nitrite+reduction
12333717632nitrate-respiration

antibiotic resistance

  • @ref: 123337
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123337
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
123337oxidase+
123337beta-galactosidase-3.2.1.23
123337alcohol dehydrogenase-1.1.1.1
123337gelatinase-
123337amylase+
123337DNase-
123337caseinase-3.4.21.50
123337catalase+1.11.1.6
123337tween esterase+
123337lecithinase-
123337lipase-
123337lysine decarboxylase-4.1.1.18
123337ornithine decarboxylase-4.1.1.17
123337protease+
123337tryptophan deaminase-
123337urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123337-+++-+-----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123337++-------++---------------+----+-+--------+++----++--+------+------+-----+-+++-++++--++++-++++-++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5100airlift reactorBotlek, area, RotterdamNetherlandsNLDEurope
59220Airlift reactorNetherlandsNLDEurope
67770Air lift reactor
123337Environment, SludgeNetherlandsNLDEurope1999

isolation source categories

  • Cat1: #Engineered

taxonmaps

  • @ref: 69479
  • File name: preview.99_976.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_64;98_148;99_976&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AY017341
  • Sequence Identity:
  • Total samples: 243
  • soil counts: 62
  • aquatic counts: 64
  • animal counts: 92
  • plant counts: 25

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
5100yes, in single cases1Risk group (German classification)
1233371Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Pseudomonas chloritidismutans 16S ribosomal RNA gene, partial sequence
  • accession: AY017341
  • length: 1501
  • database: ena
  • NCBI tax ID: 203192

Genome sequences

  • @ref: 67770
  • description: Stutzerimonas chloritidismutans AW-1
  • accession: GCA_000495915
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1263865

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.109no
flagellatedyes84.778no
gram-positiveno98.686no
anaerobicno98.001no
aerobicyes92.161no
halophileno80.692no
spore-formingno94.375no
thermophileno99.756yes
glucose-utilyes85.008no
glucose-fermentno93.419no

External links

@ref: 5100

culture collection no.: DSM 13592, KCTC 12116, CCUG 51509, ATCC BAA 443, JCM 21638, IAM 15183, NBRC 102227, CIP 107870

straininfo link

  • @ref: 82213
  • straininfo: 100885

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12508887Pseudomonas chloritidismutans sp. nov., a non-denitrifying, chlorate-reducing bacterium.Wolterink AFWM, Jonker AB, Kengen SWM, Stams AJMInt J Syst Evol Microbiol10.1099/00207713-52-6-21832002Bioreactors, Chlorates/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Microscopy, Electron, Molecular Sequence Data, Oxidation-Reduction, Phenotype, Phylogeny, Pseudomonas/*classification/genetics/isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityMetabolism
Phylogeny16166710Dechloromonas hortensis sp. nov. and strain ASK-1, two novel (per)chlorate-reducing bacteria, and taxonomic description of strain GR-1.Wolterink A, Kim S, Muusse M, Kim IS, Roholl PJM, van Ginkel CG, Stams AJM, Kengen SWMInt J Syst Evol Microbiol10.1099/ijs.0.63404-02005Chlorates/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Perchlorates/*metabolism, RNA, Ribosomal, 16S/genetics, Rhodocyclaceae/*classification/genetics/isolation & purification/metabolism, Sequence Analysis, DNA, Sewage/*microbiology, *Soil Microbiology, Species Specificity, Waste Disposal, Fluid/methods, Water Pollution, ChemicalMetabolism
Genetics16315012Genotype versus phenotype in the circumscription of bacterial species: the case of Pseudomonas stutzeri and Pseudomonas chloritidismutans.Cladera AM, Garcia-Valdes E, Lalucat JArch Microbiol10.1007/s00203-005-0052-x2005Chlorates/metabolism, Genotype, Molecular Sequence Data, Nitrates/metabolism, Phenotype, Pseudomonas/*classification/genetics/growth & development, Pseudomonas stutzeri/*classification/geneticsPhenotype
Metabolism19352644Growth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor.Mehboob F, Junca H, Schraa G, Stams AJAppl Microbiol Biotechnol10.1007/s00253-009-1985-92009Alkanes/*metabolism, Chlorates/*metabolism, Molecular Sequence Data, Nitrates/metabolism, Oxidoreductases/metabolism, Oxygen/metabolism, Pseudomonas/*growth & development/*metabolismGenetics
Metabolism23919996Structure and evolution of chlorate reduction composite transposons.Clark IC, Melnyk RA, Engelbrektson A, Coates JDmBio10.1128/mBio.00379-132013Bacteria/*genetics/*metabolism, Biotransformation, Chlorates/*metabolism, Cluster Analysis, Computational Biology, *DNA Transposable Elements, Genes, Bacterial, Metabolic Networks and Pathways/*genetics, Oxidation-Reduction, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNAPhylogeny
Metabolism25900248Genome and proteome analysis of Pseudomonas chloritidismutans AW-1(T) that grows on n-decane with chlorate or oxygen as electron acceptor.Mehboob F, Oosterkamp MJ, Koehorst JJ, Farrakh S, Veuskens T, Plugge CM, Boeren S, de Vos WM, Schraa G, Stams AJ, Schaap PJEnviron Microbiol10.1111/1462-2920.128802015Alkanes/*metabolism, Chlorates/*metabolism, Cytochrome P-450 CYP4A/genetics/metabolism, Gene Expression Profiling, Genome, Bacterial/genetics, Oxidants, Oxidation-Reduction, Oxidoreductases/genetics/metabolism, Oxygen/*metabolism, Phylogeny, Proteome/metabolism, Proteomics, Pseudomonas stutzeri/genetics/*growth & development/*metabolismProteome
Metabolism28411224Concurrent Haloalkanoate Degradation and Chlorate Reduction by Pseudomonas chloritidismutans AW-1(T).Peng P, Zheng Y, Koehorst JJ, Schaap PJ, Stams AJM, Smidt H, Atashgahi SAppl Environ Microbiol10.1128/AEM.00325-172017Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/metabolism, Biodegradation, Environmental, Chlorates/*metabolism, Halogens/*metabolism, Hydrolases/chemistry/genetics/metabolism, Molecular Sequence Data, Oxidation-Reduction, Oxygen/metabolism, Pseudomonas/chemistry/enzymology/genetics/*metabolism, Sequence AlignmentGenetics

Reference

@idauthorscataloguedoi/urltitle
5100Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13592)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13592
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34277Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5429
59220Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51509)https://www.ccug.se/strain?id=51509
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82213Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100885.1StrainInfo: A central database for resolving microbial strain identifiers
123337Curators of the CIPCollection of Institut Pasteur (CIP 107870)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107870