Archive BacDiveID:10.13145/bacdive13003.20221219.7.1

Strain identifier

BacDive ID: 13003

Type strain:

Species: Pseudomonas stutzeri

Strain Designation: AW-1

Strain history: IAM 15183 <-- KCTC 12116 <-- DSM 13592 <-- A. Wolterink AW-1.

NCBI tax ID(s): 203192 (species), 316 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 5100

BacDive-ID: 13003

DSM-Number: 13592

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, human pathogen

description: Pseudomonas stutzeri AW-1 is an aerobe, mesophilic human pathogen that was isolated from airlift reactor.

NCBI tax id

NCBI tax id	Matching level
203192	species
316	species

strain history

doi: 10.13145/bacdive13003.20221219.7.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas stutzeri
full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

synonyms

@ref	synonym
20215	Stutzerimonas chloritidismutans
20215	Pseudomonas perfectomarinus
20215	Bacterium stutzeri
20215	Pseudomonas kunmingensis
20215	Pseudomonas chloritidismutans
20215	Stutzerimonas kunmingensis
20215	Stutzerimonas stutzeri
20215	Stutzerimonas perfectomarina
20215	Pseudomonas perfectomarina

@ref: 5100

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: AW-1

type strain: no

Morphology

cell morphology

@ref	motility	confidence	gram stain
69480	yes	92.658
69480		99.565	negative

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
5100	PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM (DSMZ Medium 944)	yes	https://mediadive.dsmz.de/medium/944	Name: PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM (DSMZ Medium 944) Composition: NaHCO3 3.73 g/l Na-acetate x 3 H2O 2.72 g/l NaClO3 1.06 g/l Na2HPO4 0.53 g/l Na2S x 9 H2O 0.5 g/l NH4HCO3 0.44 g/l KH2PO4 0.41 g/l CaCl2 0.11 g/l MgCl2 0.1 g/l NaOH 0.005 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l alpha-lipoic acid 5e-05 g/l Riboflavin 5e-05 g/l Pantothenic acid 5e-05 g/l Vitamin B12 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Na2WO4 x 2 H2O 3e-05 g/l Na2SeO3 x 5 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l Nicotinic acid 2.5e-05 g/l Nicotine amide 2.5e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Pyridoxamine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
5100	R2A MEDIUM (DSMZ Medium 830)	yes	https://mediadive.dsmz.de/medium/830	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
34277	MEDIUM 72- for trypto casein soja agar	yes		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@ref	growth	type	temperature	range
5100	positive	growth	30	mesophilic
34277	positive	growth	30	mesophilic
59220	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance
5100	aerobe
5100	anaerobe
59220	aerobe

spore formation

@ref: 69480
spore formation: no
confidence: 97.8

halophily

@ref: 69480
halophily level: non-halophilic
confidence: 91.014

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
5100	airlift reactor	Botlek, area, Rotterdam	Netherlands	NLD	Europe
59220	Airlift reactor		Netherlands	NLD	Europe
67770	Air lift reactor

isolation source categories

Cat1: #Engineered

taxonmaps

@ref: 69479
File name: preview.99_976.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_64;98_148;99_976&stattab=map
Last taxonomy: Pseudomonas
16S sequence: AY017341
Sequence Identity:
Total samples: 243
soil counts: 62
aquatic counts: 64
animal counts: 92
plant counts: 25

Safety information

risk assessment

@ref: 5100
pathogenicity human: yes, in single cases
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 67770
description: Pseudomonas chloritidismutans 16S ribosomal RNA gene, partial sequence
accession: AY017341
length: 1501
database: ena
NCBI tax ID: 203192

Genome sequences

@ref: 67770
description: Stutzerimonas chloritidismutans AW-1 AW-1
accession: GCA_000495915
assembly level: contig
database: ncbi
NCBI tax ID: 1263865

External links

@ref: 5100

culture collection no.: DSM 13592, KCTC 12116, CCUG 51509, ATCC BAA 443, JCM 21638, IAM 15183, NBRC 102227

straininfo link

@ref	passport
20218	http://www.straininfo.net/strains/303530
20218	http://www.straininfo.net/strains/319983

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	12508887	Pseudomonas chloritidismutans sp. nov., a non-denitrifying, chlorate-reducing bacterium.	Wolterink AFWM, Jonker AB, Kengen SWM, Stams AJM	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2183	2002	Bioreactors, Chlorates/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Microscopy, Electron, Molecular Sequence Data, Oxidation-Reduction, Phenotype, Phylogeny, Pseudomonas/*classification/genetics/isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity	Metabolism
Phylogeny	16166710	Dechloromonas hortensis sp. nov. and strain ASK-1, two novel (per)chlorate-reducing bacteria, and taxonomic description of strain GR-1.	Wolterink A, Kim S, Muusse M, Kim IS, Roholl PJM, van Ginkel CG, Stams AJM, Kengen SWM	Int J Syst Evol Microbiol	10.1099/ijs.0.63404-0	2005	Chlorates/metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Perchlorates/metabolism, RNA, Ribosomal, 16S/genetics, Rhodocyclaceae/classification/genetics/isolation & purification/metabolism, Sequence Analysis, DNA, Sewage/microbiology, *Soil Microbiology, Species Specificity, Waste Disposal, Fluid/methods, Water Pollution, Chemical	Metabolism
Genetics	16315012	Genotype versus phenotype in the circumscription of bacterial species: the case of Pseudomonas stutzeri and Pseudomonas chloritidismutans.	Cladera AM, Garcia-Valdes E, Lalucat J	Arch Microbiol	10.1007/s00203-005-0052-x	2005	Chlorates/metabolism, Genotype, Molecular Sequence Data, Nitrates/metabolism, Phenotype, Pseudomonas/classification/genetics/growth & development, Pseudomonas stutzeri/classification/genetics	Phenotype
Metabolism	19352644	Growth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor.	Mehboob F, Junca H, Schraa G, Stams AJ	Appl Microbiol Biotechnol	10.1007/s00253-009-1985-9	2009	Alkanes/metabolism, Chlorates/metabolism, Molecular Sequence Data, Nitrates/metabolism, Oxidoreductases/metabolism, Oxygen/metabolism, Pseudomonas/growth & development/metabolism	Genetics
Metabolism	23919996	Structure and evolution of chlorate reduction composite transposons.	Clark IC, Melnyk RA, Engelbrektson A, Coates JD	mBio	10.1128/mBio.00379-13	2013	Bacteria/genetics/metabolism, Biotransformation, Chlorates/metabolism, Cluster Analysis, Computational Biology, DNA Transposable Elements, Genes, Bacterial, Metabolic Networks and Pathways/*genetics, Oxidation-Reduction, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA	Phylogeny
Metabolism	25900248	Genome and proteome analysis of Pseudomonas chloritidismutans AW-1(T) that grows on n-decane with chlorate or oxygen as electron acceptor.	Mehboob F, Oosterkamp MJ, Koehorst JJ, Farrakh S, Veuskens T, Plugge CM, Boeren S, de Vos WM, Schraa G, Stams AJ, Schaap PJ	Environ Microbiol	10.1111/1462-2920.12880	2015	Alkanes/metabolism, Chlorates/metabolism, Cytochrome P-450 CYP4A/genetics/metabolism, Gene Expression Profiling, Genome, Bacterial/genetics, Oxidants, Oxidation-Reduction, Oxidoreductases/genetics/metabolism, Oxygen/metabolism, Phylogeny, Proteome/metabolism, Proteomics, Pseudomonas stutzeri/genetics/growth & development/*metabolism	Proteome
Metabolism	28411224	Concurrent Haloalkanoate Degradation and Chlorate Reduction by Pseudomonas chloritidismutans AW-1(T).	Peng P, Zheng Y, Koehorst JJ, Schaap PJ, Stams AJM, Smidt H, Atashgahi S	Appl Environ Microbiol	10.1128/AEM.00325-17	2017	Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/metabolism, Biodegradation, Environmental, Chlorates/metabolism, Halogens/metabolism, Hydrolases/chemistry/genetics/metabolism, Molecular Sequence Data, Oxidation-Reduction, Oxygen/metabolism, Pseudomonas/chemistry/enzymology/genetics/*metabolism, Sequence Alignment	Genetics

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
5100	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13592)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-13592
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
34277	Curators of the CIP		https://brclims.pasteur.fr/brcWeb/souche/detail/1/5429
59220	Curators of the CCUG	Culture Collection University of Gothenburg (CCUG) (CCUG 51509)	https://www.ccug.se/strain?id=51509
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)