Strain identifier

BacDive ID: 12995

Type strain: No

Species: Pseudomonas stutzeri

Strain Designation: CMT.9.A

Strain history: <- D. Werner; CMT.9.A

NCBI tax ID(s): 996285 (strain), 203192 (species), 316 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1605

BacDive-ID: 12995

DSM-Number: 4166

keywords: genome sequence, Bacteria, mesophilic, motile, human pathogen

description: Pseudomonas stutzeri CMT.9.A is a mesophilic, motile human pathogen that was isolated from rhizosphere of Sorghum mutans cv. CSV5.

NCBI tax id

NCBI tax idMatching level
996285strain
203192species
316species

strain history

  • @ref: 1605
  • history: <- D. Werner; CMT.9.A

doi: 10.13145/bacdive12995.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 1605

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: CMT.9.A

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes98.735
69480100negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1605NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
1605NITROGEN-FREE MEDIUM FOR PSEUDOMONAS STUTZERI (DSMZ Medium 460)yeshttps://mediadive.dsmz.de/medium/460Name: NITROGEN-FREE MEDIUM FOR PSEUDOMONAS STUTZERI (DSMZ Medium 460) Composition: DL-Na-malate 6.6 g/l K2HPO4 0.5 g/l Yeast extract 0.2 g/l MgSO4 x 7 H2O 0.2 g/l NaCl 0.1 g/l FeCl3 x 6 H2O 0.015 g/l Distilled water

culture temp

  • @ref: 1605
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.996

compound production

  • @ref: 1605
  • compound: nitrogenase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
1605+-------+--+-+++-+--+
1605+-------+--+-+++-+--+

Isolation, sampling and environmental information

isolation

  • @ref: 1605
  • sample type: rhizosphere of Sorghum mutans cv. CSV5
  • host species: Sorghum mutans
  • country: India
  • origin.country: IND
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 1605
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas stutzeri DSM 4166 DSM 4166GCA_000195105completencbi996285
66792Pseudomonas stutzeri DSM 4166996285.3completepatric996285
66792Pseudomonas stutzeri CMT.A.9, DSM 4166651053060completeimg996285

GC content

  • @ref: 1605
  • GC-content: 66.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes90.686no
flagellatedyes88.744no
gram-positiveno98.828no
anaerobicno97.613no
aerobicyes92.083no
halophileno82.397no
spore-formingno94.959no
glucose-utilyes90.519yes
thermophileno99.806yes
glucose-fermentno92.356no

External links

@ref: 1605

culture collection no.: DSM 4166

straininfo link

  • @ref: 82205
  • straininfo: 47803

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics21515765Complete genome sequence of the nitrogen-fixing and rhizosphere-associated bacterium Pseudomonas stutzeri strain DSM4166.Yu H, Yuan M, Lu W, Yang J, Dai S, Li Q, Yang Z, Dong J, Sun L, Deng Z, Zhang W, Chen M, Ping S, Han Y, Zhan Y, Yan Y, Jin Q, Lin MJ Bacteriol10.1128/JB.05039-112011DNA, Bacterial/*chemistry/*genetics, Evolution, Molecular, *Genome, Bacterial, Molecular Sequence Data, Nitrogen/metabolism, Nitrogen Fixation, Pseudomonas stutzeri/*genetics/isolation & purification/metabolism, Rhizosphere, Sequence Analysis, DNA, Soil Microbiology, Sorghum/microbiologyMetabolism
Enzymology22226947Characterization of Halomonas sp. strain H11 alpha-glucosidase activated by monovalent cations and its application for efficient synthesis of alpha-D-glucosylglycerol.Ojima T, Saburi W, Yamamoto T, Kudo TAppl Environ Microbiol10.1128/AEM.07514-112012Amino Acid Sequence, Cations, Monovalent/*metabolism, DNA, Bacterial/chemistry/genetics, Electrophoresis, Polyacrylamide Gel, Enzyme Activators/*metabolism, Enzyme Stability, Glucosides/*metabolism, Halomonas/*enzymology/genetics, Hydrogen-Ion Concentration, Kinetics, Molecular Sequence Data, Molecular Weight, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Substrate Specificity, Temperature, alpha-Glucosidases/chemistry/genetics/*metabolismMetabolism
Enzymology24028969Monoterpenes with antibacterial activities from a Cameroonian medicinal plant Canthium Multiflorum (Rubiaceae).Kouam SF, Ngouonpe AW, Bullach A, Lamshoft M, Kuigoua GM, Spiteller MFitoterapia10.1016/j.fitote.2013.08.0252013Animals, Anti-Bacterial Agents/isolation & purification/*pharmacology, Antineoplastic Agents, Phytogenic/isolation & purification/pharmacology/therapeutic use, Antiparasitic Agents/isolation & purification/*pharmacology, Bacteria/*drug effects, Cameroon, Cell Line, Tumor, Iridoids/isolation & purification/*pharmacology, Molecular Structure, Monoterpenes/isolation & purification/*pharmacology, Neoplasms/drug therapy, Phytotherapy, Plant Components, Aerial, Plant Extracts/chemistry/*pharmacology, Plants, Medicinal, Plasmodium falciparum/drug effects, Rats, Rubiaceae/*chemistryPhylogeny
Phylogeny25251496The nitrogen-fixation island insertion site is conserved in diazotrophic Pseudomonas stutzeri and Pseudomonas sp. isolated from distal and close geographical regions.Venieraki A, Dimou M, Vezyri E, Vamvakas A, Katinaki PA, Chatzipavlidis I, Tampakaki A, Katinakis PPLoS One10.1371/journal.pone.01058372014Bacterial Proteins/genetics, Base Sequence, China, DNA, Bacterial/chemistry/genetics, Evolution, Molecular, Genetic Variation, Genomic Islands/*genetics, Geography, Germany, Greece, Models, Genetic, Molecular Sequence Data, Nitrogen Fixation/*genetics, Phylogeny, Pseudomonas/classification/*genetics, Pseudomonas stutzeri/*genetics, RNA, Ribosomal, 16S/genetics, Repetitive Sequences, Nucleic Acid/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Species SpecificityGenetics
Metabolism27233093Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria.Lidbury ID, Murphy AR, Scanlan DJ, Bending GD, Jones AM, Moore JD, Goodall A, Hammond JP, Wellington EMEnviron Microbiol10.1111/1462-2920.133902016Crops, Agricultural/growth & development/microbiology, Genomics, Phosphates/metabolism, Phosphorus/*metabolism, Proteomics, Pseudomonas fluorescens/*genetics/metabolism, Pseudomonas putida/*genetics/metabolism, Pseudomonas stutzeri/*genetics/metabolism, Regulon, Rhizosphere, *Soil MicrobiologyGenetics
Metabolism28526844Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation.Lidbury IDEA, Murphy ARJ, Fraser TD, Bending GD, Jones AME, Moore JD, Goodall A, Tibbett M, Hammond JP, Scanlan DJ, Wellington EMHSci Rep10.1038/s41598-017-02327-62017Bacterial Proteins/genetics/metabolism, Extracellular Space/metabolism, Metagenome, Metagenomics/methods, Models, Biological, Phosphoric Diester Hydrolases/genetics/*metabolism, Phosphorus/*metabolism, Pseudomonas/classification/genetics/*metabolism, *Soil MicrobiologyGenetics
Metabolism30461205Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation.Jing X, Cui Q, Li X, Yin J, Ravichandran V, Pan D, Fu J, Tu Q, Wang H, Bian X, Zhang YMicrob Biotechnol10.1111/1751-7915.133352018*Bacterial Proteins, *Biological Control Agents, *Nitrogen Fixation, Nitrogenase/metabolism, Plant Roots, *Protein Engineering, Pseudomonas, *Pseudomonas stutzeri/metabolismBiotechnology

Reference

@idauthorscataloguedoi/urltitle
1605Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4166)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4166
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82205Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47803.1StrainInfo: A central database for resolving microbial strain identifiers