Strain identifier

BacDive ID: 12852

Type strain: No

Species: Pseudomonas fluorescens

Strain Designation: 12, 216

Strain history: CIP <- 1973, Inst. Rech. Chimie Appliquée, France <- Beerens, Inst. Pasteur, Lille, France <- ATCC <- R.Y. Stanier, Inst. Pasteur, Paris, France: strain 12 <- den Dooren de Jong, Lab. Microbiol., Delft, The Netherlands: strain 216

NCBI tax ID(s): 306 (species)

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General

@ref: 12635

BacDive-ID: 12852

DSM-Number: 50091

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Pseudomonas fluorescens 12 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from tap water.

NCBI tax id

  • NCBI tax id: 306
  • Matching level: species

strain history

@refhistory
12635<- ICPB <- R.Y. Stanier; 12 <- Lab. Microbiol., Delft
119409CIP <- 1973, Inst. Rech. Chimie Appliquée, France <- Beerens, Inst. Pasteur, Lille, France <- ATCC <- R.Y. Stanier, Inst. Pasteur, Paris, France: strain 12 <- den Dooren de Jong, Lab. Microbiol., Delft, The Netherlands: strain 216

doi: 10.13145/bacdive12852.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas fluorescens
  • full scientific name: Pseudomonas fluorescens Migula 1895 (Approved Lists 1980)

@ref: 12635

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas fluorescens

full scientific name: Pseudomonas sp.

strain designation: 12, 216

type strain: no

Morphology

cell morphology

  • @ref: 119409
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 119409
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12635REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
33488MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119409CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
12635positivegrowth30mesophilic
33488positivegrowth30mesophilic
119409positivegrowth30mesophilic
119409nogrowth5psychrophilic
119409nogrowth10psychrophilic
119409nogrowth41thermophilic
119409nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119409
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate+assimilation27689
68369gluconate+assimilation24265
68369maltose-assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose+assimilation30849
68369D-glucose+assimilation17634
68369urea-hydrolysis16199
68369arginine+hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan-energy source27897
68369nitrate-reduction17632
119409citrate+carbon source16947
119409nitrate-reduction17632
119409nitrite-reduction16301
119409nitrate-respiration17632

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11940935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
119409oxidase+
119409alcohol dehydrogenase-1.1.1.1
119409catalase+1.11.1.6
119409lysine decarboxylase-4.1.1.18
119409ornithine decarboxylase-4.1.1.17
119409tryptophan deaminase-
119409urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119409--------------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
12635---+-++-+++++-++-++++
12635---+----+++++-++-++++
12635---+-+--+++++-++-++++

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119409-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119409---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
12635tap water
119409Environment, Tap water1926

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Engineered#Built environment#Water reservoir (Aquarium/pool)
#Engineered#Built environment

taxonmaps

  • @ref: 69479
  • File name: preview.99_168.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_168&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF094727
  • Sequence Identity:
  • Total samples: 5463
  • soil counts: 555
  • aquatic counts: 976
  • animal counts: 2173
  • plant counts: 1759

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
12635yes, in single casesyes1Risk group (German classification)
1194091Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas fluorescens strain ATCC 17397 16S ribosomal RNA gene, partial sequenceAF0947271417ena294
20218Pseudomonas fluorescens strain ATCC 17397 16S ribosomal RNA gene, partial sequenceJN630889791ena294

External links

@ref: 12635

culture collection no.: DSM 50091, ATCC 17397, ICPB 2657-12, CIP 73.25, IMET 11046, LMG 5916, NCIMB 10525

straininfo link

  • @ref: 82064
  • straininfo: 1871

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12635Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50091)https://www.dsmz.de/collection/catalogue/details/culture/DSM-50091
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33488Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10678
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82064Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1871.1StrainInfo: A central database for resolving microbial strain identifiers
119409Curators of the CIPCollection of Institut Pasteur (CIP 73.25)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2073.25