Strain identifier
BacDive ID: 1280
Type strain: 
Species: Bacillus coahuilensis
Strain Designation: m4-4
Strain history: <- NRRL; NRRL B-41737 <- R. Cerritos and V. Souza, Centro de Ciencias Genomicas Universidad Nacional Autonoma de Mexico; m4-4
NCBI tax ID(s): 408580 (species)
version 8.1 (current version)
General
@ref: 18058
BacDive-ID: 1280
DSM-Number: 25608
keywords: genome sequence, 16S sequence, Bacteria, moderately halophilic, spore-forming, mesophilic, Gram-positive, rod-shaped
description: Bacillus coahuilensis m4-4 is a moderately halophilic, spore-forming, mesophilic bacterium that was isolated from Chihuahuan desert lagoon, pond water.
NCBI tax id
- NCBI tax id: 408580
- Matching level: species
strain history
- @ref: 18058
- history: <- NRRL; NRRL B-41737 <- R. Cerritos and V. Souza, Centro de Ciencias Genomicas Universidad Nacional Autonoma de Mexico; m4-4
doi: 10.13145/bacdive1280.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus coahuilensis
- full scientific name: Bacillus coahuilensis Cerritos et al. 2008
@ref: 18058
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus coahuilensis
full scientific name: Bacillus coahuilensis Cerritos et al. 2008
strain designation: m4-4
type strain: yes
Morphology
cell morphology
- @ref: 23141
- gram stain: positive
- cell length: 1.5-3.0 µm
- cell width: 0.5-0.7 µm
- cell shape: rod-shaped
colony morphology
- @ref: 23141
- colony color: light yellow
- incubation period: 2 days
- medium used: marine agar 2216
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 18058 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
| 23141 | Marine agar (MA) | yes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 18058 | positive | growth | 30 | mesophilic |
| 23141 | positive | optimum | 30-37 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 23141 | positive | optimum | 7.0-8.0 | |
| 23141 | no | growth | 4.5 | |
| 23141 | positive | growth | 9.0 | alkaliphile |
Physiology and metabolism
spore formation
- @ref: 23141
- spore formation: yes
halophily
| @ref | halophily level | salt | growth | tested relation | concentration |
|---|---|---|---|---|---|
| 23141 | moderately halophilic | NaCl | positive | optimum | 5 % |
| 23141 | NaCl | positive | growth | 0.5-10.0 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 23141 | 15963 | ribitol | - | other |
| 23141 | 22599 | arabinose | - | other |
| 23141 | 17634 | D-glucose | - | other |
| 23141 | 16899 | D-mannitol | - | other |
| 23141 | 16024 | D-mannose | - | other |
| 23141 | 17924 | D-sorbitol | - | other |
| 23141 | 65327 | D-xylose | - | other |
| 23141 | 16813 | galactitol | - | other |
| 23141 | 28757 | fructose | - | other |
| 23141 | 62345 | L-rhamnose | - | other |
| 23141 | 17716 | lactose | - | other |
| 23141 | 16634 | raffinose | - | other |
| 23141 | 17814 | salicin | - | other |
| 23141 | 17992 | sucrose | - | other |
| 23141 | 17754 | glycerol | + | carbon source |
| 23141 | 28017 | starch | + | carbon source |
| 23141 | 27082 | trehalose | + | carbon source |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | isolation date |
|---|---|---|---|---|---|---|---|---|
| 18058 | Chihuahuan desert lagoon, pond water | Coahuila, Cuatro Ciénegas | Mexico | MEX | North America | |||
| 23141 | desiccation lagoon in the Churince system | western side of the Cuatro Cienegas Valley in Coahuila | Mexico | MEX | Middle and South America | 26.8472 | -102.156 | 2003-08 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Pond (small) |
| #Environmental | #Terrestrial | #Desert |
| #Environmental | #Aquatic | #Freshwater |
| #Environmental | #Aquatic | #Geologic |
taxonmaps
- @ref: 69479
- File name: preview.99_4043.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1652;97_2476;98_3064;99_4043&stattab=map
- Last taxonomy: Bacillus coahuilensis subclade
- 16S sequence: EF014452
- Sequence Identity:
- Total samples: 637
- soil counts: 265
- aquatic counts: 80
- animal counts: 161
- plant counts: 131
Safety information
risk assessment
- @ref: 18058
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 18058 | Bacillus coahuilensis strain m4-4 clone a 16S ribosomal RNA gene, partial sequence | EF014450 | 1390 | ena | 441769 |
| 18058 | Bacillus coahuilensis strain m4-4 clone b 16S ribosomal RNA gene, partial sequence | EF014451 | 1387 | ena | 441769 |
| 18058 | Bacillus coahuilensis strain m4-4 16S ribosomal RNA gene, partial sequence | EF014452 | 1451 | ena | 441769 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Bacillus coahuilensis m4-4 | GCA_000171615 | contig | ncbi | 441769 |
| 66792 | Bacillus coahuilensis m4-4 | 441769.6 | wgs | patric | 441769 |
| 66792 | Bacillus coahuilensis m4-4 | 642979338 | draft | img | 441769 |
GC content
- @ref: 23141
- GC-content: 37.0
- method: genome sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| gram-positive | yes | 90.283 | no |
| anaerobic | no | 97.864 | no |
| halophile | yes | 84.23 | yes |
| spore-forming | yes | 96.192 | yes |
| glucose-util | yes | 85.96 | no |
| aerobic | yes | 91.069 | no |
| flagellated | yes | 83.5 | no |
| thermophile | no | 98.088 | no |
| motile | yes | 84.207 | no |
| glucose-ferment | no | 92.196 | no |
External links
@ref: 18058
culture collection no.: DSM 25608, CECT 7197, NRRL B-41737
straininfo link
- @ref: 70934
- straininfo: 296479
literature
- topic: Phylogeny
- Pubmed-ID: 18398195
- title: Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Cienegas Valley in Coahuila, Mexico.
- authors: Cerritos R, Vinuesa P, Eguiarte LE, Herrera-Estrella L, Alcaraz-Peraza LD, Arvizu-Gomez JL, Olmedo G, Ramirez E, Siefert JL, Souza V
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.64959-0
- year: 2008
- mesh: Bacillus/*classification/genetics/*isolation & purification/metabolism, Base Composition, Base Sequence, Carbohydrate Metabolism, DNA Primers/genetics, DNA, Bacterial/chemistry/genetics, Genes, Bacterial, Mexico, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Salinity, Species Specificity, Water Microbiology
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 18058 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25608) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25608 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 23141 | René Cerritos, Pablo Vinuesa, Luis E. Eguiarte, Luis Herrera-Estrella, Luis D. Alcaraz-Peraza, Jackeline L. Arvizu-Gómez, Gabriela Olmedo, Enrique Ramirez, Janet L. Siefert,Valeria Souza | 10.1099/ijs.0.64959-0 | Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Cienegas Valley in Coahuila, Mexico | IJSEM 58: 919-923 2008 | 18398195 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 70934 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID296479.1 | StrainInfo: A central database for resolving microbial strain identifiers |