Name: Bacillus coahuilensis m4-4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1280

Type strain:

Species: Bacillus coahuilensis

Strain Designation: m4-4

Culture col. no.: DSM 25608, CECT 7197, NRRL B-41737

Strain history: <- NRRL; NRRL B-41737 <- R. Cerritos and V. Souza, Centro de Ciencias Genomicas Universidad Nacional Autonoma de Mexico; m4-4

NCBI tax ID(s): 408580 (species)

SI-ID 296479

NRRL B-41737, CECT 7197, LMG 24687, DSM 25608

Section

Bacillus coahuilensis m4-4 is a moderately halophilic, spore-forming, mesophilic bacterium that was isolated from Chihuahuan desert lagoon, pond water.

Gram-positive
rod-shaped
spore-forming
mesophilic
moderately halophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus coahuilensis**
	Full Scientific Name (LPSN)	Bacillus coahuilensis Cerritos et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #23141]	Gram stain	positive

[Ref.: #23141]	Cell length	1.5-3.0 µm

[Ref.: #23141]	Cell width	0.5-0.7 µm

[Ref.: #23141]	Cell shape	rod-shaped

[Ref.: #23141]	Colony color	light yellow
[Ref.: #23141]	Incubation period	2 days
[Ref.: #23141]	Cultivation medium used	marine agar 2216

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18058]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #18058]

Culture medium growth

[Ref.: #18058]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18058]

Culture medium composition

expand / minimize

[Ref.: #23141]

Culture medium

Marine agar (MA)

[Ref.: #23141]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #18058]			growth	30 °C
[Ref.: #23141]			optimum	30-37 °C

[Ref.: #18058]	Temperature range	mesophilic
[Ref.: #23141]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #23141]		optimum	7.0-8.0
[Ref.: #23141]	no	growth	4.5
[Ref.: #23141]		growth	9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23141]

Ability of spore formation

yes

[Ref.: #23141]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23141]		NaCl		optimum	5	%
[Ref.: #23141]		NaCl		growth	0.5-10.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23141]	arabinose ChEBI	-	other
[Ref.: #23141]	D-glucose ChEBI	-	other
[Ref.: #23141]	D-mannitol ChEBI	-	other
[Ref.: #23141]	D-mannose ChEBI	-	other
[Ref.: #23141]	D-sorbitol ChEBI	-	other
[Ref.: #23141]	D-xylose ChEBI	-	other
[Ref.: #23141]	fructose ChEBI	-	other
[Ref.: #23141]	galactitol ChEBI	-	other
[Ref.: #23141]	glycerol ChEBI	+	carbon source
[Ref.: #23141]	L-rhamnose ChEBI	-	other
[Ref.: #23141]	lactose ChEBI	-	other
[Ref.: #23141]	raffinose ChEBI	-	other
[Ref.: #23141]	ribitol ChEBI	-	other
[Ref.: #23141]	salicin ChEBI	-	other
[Ref.: #23141]	starch ChEBI	+	carbon source
[Ref.: #23141]	sucrose ChEBI	-	other
[Ref.: #23141]	trehalose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18058]	Sample type/isolated from	Chihuahuan desert lagoon, pond water
[Ref.: #18058]	Geographic location (country and/or sea, region)	Coahuila, Cuatro Ciénegas
[Ref.: #18058]	Country	Mexico
[Ref.: #18058]	Country ISO 3 Code	MEX
[Ref.: #18058]	Continent	North America

[Ref.: #23141]	Sample type/isolated from	desiccation lagoon in the Churince system
[Ref.: #23141]	Geographic location (country and/or sea, region)	western side of the Cuatro Cienegas Valley in Coahuila
[Ref.: #23141]	Country	Mexico
[Ref.: #23141]	Country ISO 3 Code	MEX
[Ref.: #23141]	Continent	Middle and South America
[Ref.: #23141]	Geographic location	26.8472°/-102.1560°
[Ref.: #23141]	Isolation date	2003-08
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Pond (small)
#Environmental	#Terrestrial	#Desert
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF014452 (>99% sequence identity) for Bacillus coahuilensis subclade from Microbeatlas

Aquatic Samples	80
Soil Samples	265
Animal Samples	161
Plant Samples	131
Total Samples	637

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18058]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18058]	Bacillus coahuilensis strain m4-4 clone a 16S ribosomal RNA gene, partial sequence	EF014450	1390	ENA	441769 tax ID
[Ref.: #18058]	Bacillus coahuilensis strain m4-4 clone b 16S ribosomal RNA gene, partial sequence	EF014451	1387	ENA	441769 tax ID
[Ref.: #18058]	Bacillus coahuilensis strain m4-4 16S ribosomal RNA gene, partial sequence	EF014452	1451	ENA	441769 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus coahuilensis m4-4	GCA_000171615	contig	GenBank	441769 tax ID	*
[Ref.: #66792]	Bacillus coahuilensis m4-4	441769.6	wgs	PATRIC	441769 tax ID	*
[Ref.: #66792]	Bacillus coahuilensis m4-4	642979338	draft	JGI IMG/M	441769 tax ID	*

[Ref.: #23141]

GC-content

37.0 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus coahuilensis m4-4 PATRIC: 441769.6
[Ref.: #69480]	gram-positive	yes	90.283	no
[Ref.: #69480]	anaerobic	no	97.864	no
[Ref.: #69480]	halophile	yes	84.23	yes
[Ref.: #69480]	spore-forming	yes	96.192	yes
[Ref.: #69480]	glucose-util	yes	85.96	no
[Ref.: #69480]	aerobic	yes	91.069	no
[Ref.: #69480]	flagellated	yes	83.5	no
[Ref.: #69480]	thermophile	no	98.088	no
[Ref.: #69480]	motile	yes	84.207	no
[Ref.: #69480]	glucose-ferment	no	92.196	no

Availability in culture collections External links

[Ref.: #18058]

Culture collection no.

DSM 25608, CECT 7197, NRRL B-41737

[Ref.: #70934]

SI-ID 296479

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Cienegas Valley in Coahuila, Mexico.	Cerritos R, Vinuesa P, Eguiarte LE, Herrera-Estrella L, Alcaraz-Peraza LD, Arvizu-Gomez JL, Olmedo G, Ramirez E, Siefert JL, Souza V	Int J Syst Evol Microbiol	10.1099/ijs.0.64959-0	2008	*

References

#18058

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25608

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23141

René Cerritos, Pablo Vinuesa, Luis E. Eguiarte, Luis Herrera-Estrella, Luis D. Alcaraz-Peraza, Jackeline L. Arvizu-Gómez, Gabriela Olmedo, Enrique Ramirez, Janet L. Siefert,Valeria Souza: Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Cienegas Valley in Coahuila, Mexico. IJSEM 58: 919 - 923 2008 ( DOI 10.1099/ijs.0.64959-0 , PubMed 18398195 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70934

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID296479.1 )

* These data were automatically processed and therefore are not curated

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