Strain identifier

BacDive ID: 12690

Type strain: Yes

Species: Pseudoalteromonas flavipulchra

Strain history: CIP <- 2002, NCIMB <- M.J. Gauthier, Nice, France

NCBI tax ID(s): 1315273 (strain), 137779 (species)

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General

@ref: 5338

BacDive-ID: 12690

DSM-Number: 14401

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, motile

description: Pseudoalteromonas flavipulchra DSM 14401 is an obligate aerobe, mesophilic, motile bacterium that was isolated from seawater.

NCBI tax id

NCBI tax idMatching level
1315273strain
137779species

strain history

@refhistory
5338<- E. P. Ivanova <- NCIMB
119379CIP <- 2002, NCIMB <- M.J. Gauthier, Nice, France

doi: 10.13145/bacdive12690.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Pseudoalteromonadaceae
  • genus: Pseudoalteromonas
  • species: Pseudoalteromonas flavipulchra
  • full scientific name: Pseudoalteromonas flavipulchra Ivanova et al. 2002

@ref: 5338

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Pseudoalteromonadaceae

genus: Pseudoalteromonas

species: Pseudoalteromonas flavipulchra

full scientific name: Pseudoalteromonas flavipulchra Ivanova et al. 2002

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes97.759
6948099.975negative
119379yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5338BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
42042Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
119379CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5338positivegrowth28mesophilic
42042positivegrowth30mesophilic
119379positivegrowth25-37mesophilic
119379nogrowth5psychrophilic
119379nogrowth10psychrophilic
119379nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119379
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.69
119379no

halophily

@refsaltgrowthtested relationconcentration
119379NaClpositivegrowth2-8 %
119379NaClnogrowth0 %
119379NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1193794853esculin-hydrolysis
11937917632nitrate-reduction
11937916301nitrite-reduction
11937917632nitrate-respiration

antibiotic resistance

  • @ref: 119379
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119379
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
119379oxidase+
119379beta-galactosidase-3.2.1.23
119379alcohol dehydrogenase-1.1.1.1
119379gelatinase+
119379amylase+
119379DNase+
119379caseinase+3.4.21.50
119379catalase+1.11.1.6
119379tween esterase+
119379lecithinase+
119379lipase+
119379lysine decarboxylase-4.1.1.18
119379ornithine decarboxylase-4.1.1.17
119379protease-
119379tryptophan deaminase-
119379urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119379-+++-+--+-++-----+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119379+--++--+-++--------------------------------------+---------+-------------------++-----+---++-++-++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5338seawaterNiceFranceFRAEurope
119379Environment, SeawaterNiceFranceFRAEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_6623.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_57;96_353;97_393;98_4884;99_6623&stattab=map
  • Last taxonomy: Pseudoalteromonas
  • 16S sequence: AF297958
  • Sequence Identity:
  • Total samples: 1509
  • soil counts: 33
  • aquatic counts: 1221
  • animal counts: 253
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53381Risk group (German classification)
1193791Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5338
  • description: Pseudoalteromonas flavipulchra 16S ribosomal RNA gene, partial sequence
  • accession: AF297958
  • length: 1483
  • database: ena
  • NCBI tax ID: 137779

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudoalteromonas flavipulchra LMG 20361GCA_014596995contigncbi137779
66792Pseudoalteromonas flavipulchra NCIMB 2033 = ATCC BAA-314GCA_014858715contigncbi1315273
66792Pseudoalteromonas flavipulchra NCIMB 2033 = ATCC BAA-3141315273.3wgspatric1315273
66792Pseudoalteromonas flavipulchra NCIMB 2033 = ATCC BAA-3141315273.7wgspatric1315273
66792Pseudoalteromonas flavipulchra strain LMG 20361137779.9wgspatric137779
66792Pseudoalteromonas flavipulchra NCIMB 2033 = ATCC BAA-3142630968884draftimg1315273

GC content

  • @ref: 5338
  • GC-content: 41.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes89.633no
gram-positiveno99.211no
anaerobicno97.723no
halophileyes63.169no
spore-formingno96.594no
glucose-utilyes92.593no
flagellatedyes88.822no
thermophileno98.867yes
aerobicyes91.478no
glucose-fermentno79.076no

External links

@ref: 5338

culture collection no.: DSM 14401, KMM 3630, LMG 20361, NCIMB 2033, CIP 107428

straininfo link

  • @ref: 81902
  • straininfo: 14997

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11837311Pseudoalteromonas maricaloris sp. nov., isolated from an Australian sponge, and reclassification of [Pseudoalteromonas aurantia] NCIMB 2033 as Pseudoalteromonas flavipulchra sp. nov.Ivanova EP, Shevchenko LS, Sawabe T, Lysenko AM, Svetashev VI, Gorshkova NM, Satomi M, Christen R, Mikhailov VVInt J Syst Evol Microbiol10.1099/00207713-52-1-2632002Animals, Australia, Base Composition, DNA, Ribosomal/analysis, Fatty Acids/analysis, Gammaproteobacteria/*classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phospholipids/analysis, Phylogeny, Porifera/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
12559750Structure of an acidic polysaccharide from the marine bacterium Pseudoalteromonas flavipulchra NCIMB 2033(T).Muldoon J, Perepelov AV, Shashkov AS, Nazarenko EL, Zubkov VA, Gorshkova RP, Ivanova EP, Gorshkova NM, Knirel YA, Savage AVCarbohydr Res10.1016/s0008-6215(02)00483-42003Deoxy Sugars/chemistry, Galactose/chemistry, Hexoses/chemistry, Magnetic Resonance Spectroscopy, Polysaccharides/*chemistry, Pseudoalteromonas/*chemistry, Sugar Acids/chemistry
Enzymology35323464Identification and Characterization of Three Chitinases with Potential in Direct Conversion of Crystalline Chitin into N,N'-diacetylchitobiose.Ren XB, Dang YR, Liu SS, Huang KX, Qin QL, Chen XL, Zhang YZ, Wang YJ, Li PYMar Drugs10.3390/md200301652022Bacterial Proteins/*chemistry/isolation & purification, Chitin/*chemistry, Chitinases/*chemistry/isolation & purification, Disaccharides/*chemistry, Genome, Bacterial, Pseudoalteromonas/*enzymology/genetics, Sodium Chloride/chemistryPhylogeny

Reference

@idauthorscataloguedoi/urltitle
5338Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14401)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14401
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
42042Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4863
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81902Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14997.1StrainInfo: A central database for resolving microbial strain identifiers
119379Curators of the CIPCollection of Institut Pasteur (CIP 107428)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107428