Strain identifier
BacDive ID: 12637
Type strain: 
Species: Cutibacterium acnes
Strain history: <- P. Dürre; KPA171202 <- S. Hujer
NCBI tax ID(s): 1747 (species)
version 8.1 (current version)
General
@ref: 6416
BacDive-ID: 12637
DSM-Number: 16379
keywords: genome sequence, Bacteria, anaerobe, mesophilic
description: Cutibacterium acnes DSM 16379 is an anaerobe, mesophilic bacterium that was isolated from contamination of an anaerobic culture.
NCBI tax id
- NCBI tax id: 1747
- Matching level: species
strain history
- @ref: 6416
- history: <- P. Dürre; KPA171202 <- S. Hujer
doi: 10.13145/bacdive12637.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Propionibacteriales
- family: Propionibacteriaceae
- genus: Cutibacterium
- species: Cutibacterium acnes
- full scientific name: Cutibacterium acnes (Gilchrist 1900) Scholz and Kilian 2016
synonyms
@ref synonym 20215 Bacillus acnes 20215 Propionibacterium acnes
@ref: 6416
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Propionibacteriaceae
genus: Cutibacterium
species: Cutibacterium acnes
full scientific name: Cutibacterium acnes (Gilchrist 1900) Scholz and Kilian 2016 emend. Nouioui et al. 2018
type strain: no
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 94.665 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 6416 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
| 6416 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
culture temp
- @ref: 6416
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 6416
- oxygen tolerance: anaerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.994 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68380 | 16199 | urea | - | hydrolysis |
| 68380 | 16634 | raffinose | - | fermentation |
| 68380 | 29985 | L-glutamate | - | degradation |
| 68380 | 17632 | nitrate | + | reduction |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68380 | glutamyl-glutamate arylamidase | - | |
| 68380 | histidine arylamidase | - | |
| 68380 | tyrosine arylamidase | - | |
| 68380 | leucine arylamidase | - | 3.4.11.1 |
| 68380 | phenylalanine arylamidase | - | |
| 68380 | proline-arylamidase | + | 3.4.11.5 |
| 68380 | alkaline phosphatase | - | 3.1.3.1 |
| 68380 | alpha-fucosidase | - | 3.2.1.51 |
| 68380 | glutamate decarboxylase | - | 4.1.1.15 |
| 68380 | alpha-arabinosidase | - | 3.2.1.55 |
| 68380 | beta-glucosidase | - | 3.2.1.21 |
| 68380 | alpha-glucosidase | + | 3.2.1.20 |
| 68380 | beta-Galactosidase 6-phosphate | - | |
| 68380 | alpha-galactosidase | - | 3.2.1.22 |
| 68380 | urease | - | 3.5.1.5 |
API rID32A
| @ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 6416 | - | - | - | +/- | - | + | - | - | - | + | + | - | - | - | + | + | - | +/- | + | - | - | - | +/- | - | + | + | - | - | + |
| 6416 | - | + | - | +/- | - | + | - | - | - | +/- | + | - | - | - | + | + | - | - | + | - | - | - | - | - | + | + | - | - | + |
| 6416 | - | + | - | - | - | + | - | - | - | + | + | - | - | - | + | + | - | - | + | - | - | - | - | - | + | + | - | - | + |
| 6416 | - | + | - | + | - | + | - | - | - | + | + | - | - | - | + | +/- | - | + | + | - | - | - | - | - | + | + | - | - | + |
| 6416 | - | + | - | + | - | + | - | - | +/- | +/- | - | - | - | - | + | - | - | + | + | +/- | - | - | - | - | + | + | - | - | + |
| 6416 | - | - | - | +/- | - | + | - | - | + | + | + | - | - | - | + | - | - | +/- | + | - | - | - | - | - | +/- | +/- | - | - | +/- |
Isolation, sampling and environmental information
isolation
- @ref: 6416
- sample type: contamination of an anaerobic culture
- geographic location: Ulm
- country: Germany
- origin.country: DEU
- continent: Europe
- latitude: 48.4011
- longitude: 9.9876
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | |
| #Engineered | #Laboratory | #Lab enrichment |
| #Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 6416
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
- @ref: 66792
- description: Cutibacterium acnes KPA171202
- accession: 267747.46
- assembly level: complete
- database: patric
- NCBI tax ID: 267747
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 94.078 | no |
| gram-positive | yes | 90.304 | no |
| anaerobic | yes | 60.386 | yes |
| halophile | no | 84.005 | no |
| spore-forming | no | 90.986 | no |
| glucose-util | yes | 90.767 | no |
| flagellated | no | 98.206 | no |
| thermophile | no | 78.719 | yes |
| aerobic | no | 87.833 | yes |
| glucose-ferment | yes | 82.678 | no |
External links
@ref: 6416
culture collection no.: DSM 16379, KPA 171202
straininfo link
- @ref: 81850
- straininfo: 215483
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 18201989 | A new phylogenetic group of Propionibacterium acnes. | McDowell A, Perry AL, Lambert PA, Patrick S | J Med Microbiol | 10.1099/jmm.0.47489-0 | 2008 | Antibodies, Bacterial/metabolism, Antibodies, Monoclonal/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Gram-Positive Bacterial Infections/*microbiology, Humans, Microscopy, Fluorescence, Molecular Sequence Data, Phylogeny, Polymorphism, Genetic, Propionibacterium acnes/*classification/cytology/*genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Serotyping | Metabolism |
| Enzymology | 19132369 | Characterization of beta-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes. | Nakajima M, Nishimoto M, Kitaoka M | Appl Microbiol Biotechnol | 10.1007/s00253-008-1838-y | 2009 | Acetylglucosamine/analogs & derivatives/metabolism, Cloning, Molecular, DNA, Bacterial/chemistry/genetics, Disaccharides/metabolism, Enzyme Stability, Galactosyltransferases/*genetics/*metabolism, Hydrogen-Ion Concentration, Kinetics, Molecular Sequence Data, Phylogeny, Propionibacterium acnes/*enzymology/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Substrate Specificity, Temperature | Metabolism |
| Enzymology | 19194001 | Expression, purification, crystallization and preliminary X-ray diffraction analysis of the soluble domain of PPA0092, a putative nitrite reductase from Propionibacterium acnes. | Nojiri M, Shirota F, Hira D, Suzuki S | Acta Crystallogr Sect F Struct Biol Cryst Commun | 10.1107/S1744309108040207 | 2009 | Amino Acid Sequence, Anaerobiosis, Bacterial Proteins/*biosynthesis/*chemistry/genetics, Gene Expression Regulation, Bacterial/*physiology, Molecular Sequence Data, Nitrates/chemistry/metabolism, Nitrite Reductases/*biosynthesis/*chemistry/genetics, Nitrogen/chemistry/metabolism, Oxygen Consumption, Propionibacterium acnes/*chemistry/genetics, Protein Structure, Tertiary/physiology | Genetics |
| Genetics | 21738717 | Comparative genomics and transcriptomics of Propionibacterium acnes. | Brzuszkiewicz E, Weiner J, Wollherr A, Thurmer A, Hupeden J, Lomholt HB, Kilian M, Gottschalk G, Daniel R, Mollenkopf HJ, Meyer TF, Bruggemann H | PLoS One | 10.1371/journal.pone.0021581 | 2011 | Bacterial Proteins/*genetics, Genomics/*methods, Propionibacterium acnes/*genetics, Transcriptome/*genetics | Transcriptome |
| Genetics | 23762865 | Analysis of complete genomes of Propionibacterium acnes reveals a novel plasmid and increased pseudogenes in an acne associated strain. | Kasimatis G, Fitz-Gibbon S, Tomida S, Wong M, Li H | Biomed Res Int | 10.1155/2013/918320 | 2013 | Acne Vulgaris/*microbiology, Chromosome Inversion, Chromosomes, Bacterial/genetics, DNA, Circular/genetics, Genes, Bacterial/genetics, Genetic Loci/genetics, Genome, Bacterial/*genetics, Genomic Islands/genetics, Humans, Molecular Sequence Annotation, Molecular Sequence Data, Plasmids/*genetics, Propionibacterium acnes/*genetics, Pseudogenes/*genetics, Sequence Homology, Nucleic Acid | Pathogenicity |
| Genetics | 28602804 | In vitro emergence of fluoroquinolone resistance in Cutibacterium (formerly Propionibacterium) acnes and molecular characterization of mutations in the gyrA gene. | Takoudju EM, Guillouzouic A, Kambarev S, Pecorari F, Corvec S | Anaerobe | 10.1016/j.anaerobe.2017.06.005 | 2017 | Anti-Bacterial Agents/*pharmacology, Bacteriological Techniques, DNA Gyrase/*genetics, *Drug Resistance, Bacterial, Fluoroquinolones/*pharmacology, Genotype, Microbial Sensitivity Tests, Mutation Rate, *Mutation, Missense, Propionibacterium acnes/*drug effects/*genetics, Selection, Genetic, Sequence Analysis, DNA | Transcriptome |
| 30647611 | In silico prediction and qPCR validation of novel sRNAs in Propionibacterium acnes KPA171202. | Balgir PP, Dhiman SR, Kaur P | J Genet Eng Biotechnol | 10.1016/j.jgeb.2016.03.002 | 2016 | |||
| Genetics | 32675190 | Complete Genome Sequences and Methylome Analyses of Cutibacterium acnes subsp. acnes Strains DSM 16379 and DSM 1897(T). | Deptula P, Laine P, Paulin L, Auvinen P, Roberts RJ, Johnston CD, Varmanen P | Microbiol Resour Announc | 10.1128/MRA.00705-20 | 2020 | ||
| Pathogenicity | 35344565 | Engineering selectivity of Cutibacterium acnes phages by epigenetic imprinting. | Knodlseder N, Nevot G, Fabrega MJ, Mir-Pedrol J, Sanvicente-Garcia M, Campama-Sanz N, Paetzold B, Lood R, Guell M | PLoS Pathog | 10.1371/journal.ppat.1010420 | 2022 | *Acne Vulgaris/genetics/microbiology, *Bacteriophages/genetics, Epigenesis, Genetic, Humans, Propionibacterium acnes/genetics, Skin/microbiology | Biotechnology |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 6416 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16379) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16379 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 68380 | Automatically annotated from API rID32A | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 81850 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID215483.1 | StrainInfo: A central database for resolving microbial strain identifiers |