Strain identifier

BacDive ID: 12602

Type strain: Yes

Species: Promicromonospora vindobonensis

Strain Designation: V-45, V45

Strain history: CIP <- 2003, CCM <- H.J. Busse, Vienna Univ., Vienna, Austria <- C. Zlamala: strain V45

NCBI tax ID(s): 195748 (species)

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General

@ref: 6199

BacDive-ID: 12602

DSM-Number: 15942

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Promicromonospora vindobonensis V-45 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from air in the medieval "Virgilkapelle".

NCBI tax id

  • NCBI tax id: 195748
  • Matching level: species

strain history

@refhistory
6199<- P. Kämpfer; V-45 <- H. J. Busse <- C. Zlamala
67770CCM 7044 <-- H.-J. Busse V45 <-- C. Zlamala.
123845CIP <- 2003, CCM <- H.J. Busse, Vienna Univ., Vienna, Austria <- C. Zlamala: strain V45

doi: 10.13145/bacdive12602.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Promicromonosporaceae
  • genus: Promicromonospora
  • species: Promicromonospora vindobonensis
  • full scientific name: Promicromonospora vindobonensis Busse et al. 2003

@ref: 6199

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Promicromonosporaceae

genus: Promicromonospora

species: Promicromonospora vindobonensis

full scientific name: Promicromonospora vindobonensis Busse et al. 2003

strain designation: V-45, V45

type strain: yes

Morphology

cell morphology

  • @ref: 123845
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 123845

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6199TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33208MEDIUM 464 - for Citricoccus muralisyesDistilled water make up to (1000.000 ml);Sodium chloride (5.000 g);Agar(20.000 g);Peptone (10.000 g);Beef extract (10.000 g)
123845CIP Medium 464yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=464

culture temp

@refgrowthtypetemperaturerange
6199positivegrowth28mesophilic
33208positivegrowth30mesophilic
67770positivegrowth28mesophilic
123845positivegrowth25-37mesophilic
123845nogrowth10psychrophilic
123845nogrowth41thermophilic
123845nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123845
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
123845NaClpositivegrowth0-4 %
123845NaClnogrowth6 %
123845NaClnogrowth8 %
123845NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12384516947citrate-carbon source
1238454853esculin+hydrolysis
123845606565hippurate+hydrolysis
12384517632nitrate+reduction
12384516301nitrite-reduction
12384517632nitrate-respiration

antibiotic resistance

  • @ref: 123845
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123845
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12384515688acetoin-
12384517234glucose-

enzymes

@refvalueactivityec
123845oxidase-
123845beta-galactosidase+3.2.1.23
123845alcohol dehydrogenase-1.1.1.1
123845gelatinase+/-
123845amylase+
123845DNase+
123845caseinase+3.4.21.50
123845catalase+1.11.1.6
123845tween esterase-
123845gamma-glutamyltransferase+2.3.2.2
123845lecithinase-
123845lipase-
123845lysine decarboxylase-4.1.1.18
123845ornithine decarboxylase-4.1.1.17
123845phenylalanine ammonia-lyase-4.3.1.24
123845protease+
123845tryptophan deaminase-
123845urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123845-+++-+--+---++-++++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123845+-+++--+-+++++-++++++++++-+----+-+++---+---+-----+----+-++-++--------------+-+-++-+---+-+-+----+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6199air in the medieval "Virgilkapelle"ViennaAustriaAUTEurope
67770Air in the 'Virgilkapelle' chapelViennaAustriaAUTEurope
123845Environment, Air in the chapelViennaAustriaAUTEurope1999

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air
  • Cat3: #Indoor Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_69467.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1939;97_2331;98_2876;99_69467&stattab=map
  • Last taxonomy: Promicromonospora
  • 16S sequence: AJ487302
  • Sequence Identity:
  • Total samples: 171
  • soil counts: 93
  • animal counts: 5
  • plant counts: 73

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
61991Risk group (German classification)
1238451Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6199
  • description: Promicromonospora vindobonesis partial 16S rRNA gene, type strain V-45T
  • accession: AJ487302
  • length: 1342
  • database: ena
  • NCBI tax ID: 195748

GC content

@refGC-contentmethod
619970
6777070high performance liquid chromatography (HPLC)

External links

@ref: 6199

culture collection no.: DSM 15942, CCM 7044, JCM 14120, CIP 108164, IFO 16525, NBRC 16525

straininfo link

  • @ref: 81816
  • straininfo: 88109

literature

  • topic: Phylogeny
  • Pubmed-ID: 13130039
  • title: Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval 'Virgilkapelle' in Vienna.
  • authors: Busse HJ, Zlamala C, Buczolits S, Lubitz W, Kampfer P, Takeuchi M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02522-0
  • year: 2003
  • mesh: Actinobacteria/*classification/genetics/*isolation & purification/metabolism, Air Microbiology, Austria, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
6199Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15942)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15942
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33208Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5756
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81816Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88109.1StrainInfo: A central database for resolving microbial strain identifiers
123845Curators of the CIPCollection of Institut Pasteur (CIP 108164)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108164