Strain identifier

BacDive ID: 12584

Type strain: Yes

Species: Isoptericola halotolerans

Strain history: <- WJ Li, Yunnan Univ., China

NCBI tax ID(s): 300560 (species)

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General

@ref: 6413

BacDive-ID: 12584

DSM-Number: 16376

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Isoptericola halotolerans DSM 16376 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 300560
  • Matching level: species

strain history

@refhistory
6413<- Wen-Jun Li
67770KCTC 19046 <-- W.-J. Li YIM 70177.
67771<- WJ Li, Yunnan Univ., China

doi: 10.13145/bacdive12584.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Promicromonosporaceae
  • genus: Isoptericola
  • species: Isoptericola halotolerans
  • full scientific name: Isoptericola halotolerans Zhang et al. 2005

@ref: 6413

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Promicromonosporaceae

genus: Isoptericola

species: Isoptericola halotolerans

full scientific name: Isoptericola halotolerans Zhang et al. 2005

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
67771positive
69480no90.682
69480positive100

multicellular morphology

@refforms multicellular complexmedium name
19740noISP 2
19740noISP 3
19740noISP 4
19740noISP 5
19740noISP 6
19740noISP 7

Culture and growth conditions

culture medium

  • @ref: 6413
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6413positivegrowth28mesophilic
19740positiveoptimum28mesophilic
67770positivegrowth28mesophilic
67771positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.586

halophily

  • @ref: 19740
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 10 %

murein

  • @ref: 6413
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1974062968cellulose+
1974016634raffinose+
1974026546rhamnose+
1974028757fructose+
1974029864mannitol+
1974017268myo-inositol-
1974018222xylose-
1974017992sucrose+
1974022599arabinose+
1974017234glucose+

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19740+++-+--+++-++-++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6413soilQinghai ProvinceChinaCHNAsia
67770SoilQinghai ProvinceChinaCHNAsia
67771From saline soilQinghai ProvinceChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64131Risk group (German classification)
197401Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Isoptericola halotolerans strain YIM 70177 16S ribosomal RNA gene, partial sequence
  • accession: AY789835
  • length: 1382
  • database: ena
  • NCBI tax ID: 300560

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Isoptericola halotolerans KCTC 19046GCA_013149785scaffoldncbi300560
66792Isoptericola halotolerans strain KCTC 19046300560.7wgspatric300560
66792Isoptericola halotolerans KCTC 190462849278078draftimg300560

GC content

@refGC-contentmethod
641372.8
6777072.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes52no
flagellatedno97.247no
gram-positiveyes89.217yes
anaerobicno99.43yes
aerobicyes92.955yes
halophileno69.976no
spore-formingno78.9no
glucose-utilyes90.311no
thermophileno99.237no
motileno90.515no
glucose-fermentno79.699no

External links

@ref: 6413

culture collection no.: DSM 16376, KCTC 19046, YIM 70177, JCM 13590, NBRC 104116, CIP 108968

straininfo link

  • @ref: 81798
  • straininfo: 133635

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16166680Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China.Zhang YQ, Schumann P, Li WJ, Chen GZ, Tian XP, Stackebrandt E, Xu LH, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.63641-02005Actinomycetales/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, *Soil MicrobiologyGenetics
Phylogeny33475779Isoptericola sediminis sp. nov., Isolated from Chilika Lagoon.Kumar D, U J, A K, G S, Sasikala C, Ramana CVCurr Microbiol10.1007/s00284-020-02325-42021Actinobacteria, Bacterial Typing Techniques, DNA, Bacterial/genetics, *Fatty Acids/analysis, India, Nucleic Acid Hybridization, *Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6413Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16376)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16376
19740Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM16367.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
81798Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID133635.1StrainInfo: A central database for resolving microbial strain identifiers