Isoptericola halotolerans (DSM 16376, KCTC 19046, YIM 70177)

Name: Isoptericola halotolerans (DSM 16376, KCTC 19046, YIM 70177)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12584

Type strain

Culture col. no. DSM 16376 KCTC 19046 YIM 70177 NBRC 104116 JCM 13590 1 more

NCBI tax ID(s) 300560

Special Collections DSMZ JCM KCTC CIP Wink compendium

History <- Wen-Jun Li KCTC 19046 <-- W.-J. Li YIM 70177. <- WJ Li, Yunnan Univ., China

Links

Isoptericola halotolerans DSM 16376 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from soil.

Gram-positive aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 133635

DSM 16376,KCTC 19046,JCM 13590,NBRC 104116

@ref 20215

Last LPSN update 2025-12-16 09:49:53

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Promicromonosporaceae

Genus Isoptericola

Species Isoptericola halotolerans

Full scientific name Isoptericola halotolerans Zhang et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Confidence
67771	positive
125439	positive	99.8
125438		92.5
125438	positive	91.309

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19740		ISP 2
19740		ISP 3
19740		ISP 4
19740		ISP 5
19740		ISP 6
19740		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6413	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6413	positive	growth	28	mesophilic
19740	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic
67771	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
67771	aerobe
125439	obligate aerobe	98.9

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19740	NaCl	positive	maximum	10 %

Murein

@ref	Murein short key	Type
6413	A11.31	A4alpha L-Lys-D-Asp

Observation

67770

Observationquinones: MK-9(H₄), MK-9(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
19740	22599 ChEBI	arabinose	+
19740	62968 ChEBI	cellulose	+
19740	28757 ChEBI	fructose	+
19740	17234 ChEBI	glucose	+
19740	29864 ChEBI	mannitol	+
19740	17268 ChEBI	myo-inositol	-
19740	16634 ChEBI	raffinose	+
19740	26546 ChEBI	rhamnose	+
19740	17992 ChEBI	sucrose	+
19740	18222 ChEBI	xylose	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	+	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19740	not determinedn.d.	+	+	+	-	+	-	-	+	+	+	-	+	+	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6413	soil	Qinghai Province	China	CHN	Asia
67770	Soil	Qinghai Province	China	CHN	Asia
67771	From saline soil	Qinghai Province	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6413	1	Risk group (German classification) According to TRBA 466
19740	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1314978v1 assembly for Isoptericola halotolerans KCTC 19046	scaffold	GCA_013149785	300560.7	2849278078	300560	67.4
124043	ASM3952925v1 assembly for Isoptericola halotolerans JCM 13590	scaffold	GCA_039529255			300560	13.22

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Isoptericola halotolerans strain YIM 70177 16S ribosomal RNA gene, partial sequence	AY789835	1382	300560
124043	Isoptericola halotolerans gene for 16S rRNA, partial sequence, strain: NBRC 104116.	AB489222	1481	300560
124043	Isoptericola halotolerans strain YIM 70177 16S ribosomal RNA gene, partial sequence.	MT539304	1300	300560

GC content

@ref	GC-content (mol%)	Method
6413	72.8
67770	72.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Isoptericola halotolerans KCTC 19046
NCBI: GCA_013149785

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	51.80	no
125439	motility	BacteriaNetⓘ	no	79.20	no
125439	gram_stain	BacteriaNetⓘ	positive	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Isoptericola halotolerans KCTC 19046
NCBI: GCA_013149785.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.31	yes
125438	anaerobic	anaerobicⓘ	no	96.22	yes
125438	aerobic	aerobicⓘ	yes	88.35	yes
125438	spore-forming	spore-formingⓘ	no	56.87	no
125438	thermophilic	thermophileⓘ	no	95.50	yes
125438	flagellated	motile2+ⓘ	no	92.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genomic analysis of Isoptericola halotolerans SM2308 reveals its potential involved in fucoidan degradation.	Zhang YQ, Yuan Q, Liu JQ, Liang XC, Wang JP, Jiang WX, Li PY.	Mar Genomics	10.1016/j.margen.2025.101165	2025
	Insight into Bacterial Community Responses to Polycyclic Aromatic Hydrocarbons and the Degradation Potentials of Three Bacterial Isolates in Seagrass Halophila ovalis Sediments.	Ahmad M, Ling J, Yang Q, Sajjad W, Zhou W, Yin J, Dong J.	Curr Microbiol	10.1007/s00284-021-02670-y	2021
Genetics	Genome Analysis of a Potential Novel Vibrio Species Secreting pH- and Thermo-Stable Alginate Lyase and Its Application in Producing Alginate Oligosaccharides.	Bao K, Yang M, Sun Q, Zhang K, Huang H.	Mar Drugs	10.3390/md22090414	2024
Enzymology	Biochemical Characterization and Degradation Pattern of a Novel Endo-Type Bifunctional Alginate Lyase AlyA from Marine Bacterium Isoptericola halotolerans.	Zhu B, Ning L, Jiang Y, Ge L.	Mar Drugs	10.3390/md16080258	2018
	Purification and Characterization of a Novel Alginate Lyase from a Marine Streptomyces Species Isolated from Seaweed.	Nguyen TNT, Chataway T, Araujo R, Puri M, Franco CMM.	Mar Drugs	10.3390/md19110590	2021
	A novel marine bacterium Isoptericola sp. JS-C42 with the ability to saccharifying the plant biomasses for the aid in cellulosic ethanol production.	Santhi VS, Gupta A, Saranya S, Jebakumar SRD.	Biotechnol Rep (Amst)	10.1016/j.btre.2014.05.002	2014
	Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria.	Dragojevic M, Stankovic N, Djokic L, Raicevic V, Jovicic-Petrovic J.	Environ Microbiome	10.1186/s40793-023-00477-x	2023
Metabolism	Purification and Characterization of a Novel Endolytic Alginate Lyase from Microbulbifer sp. SH-1 and Its Agricultural Application.	Yang J, Cui D, Chen D, Chen W, Ma S, Shen H.	Mar Drugs	10.3390/md18040184	2020
	Screening of alginate lyase-excreting microorganisms from the surface of brown algae.	Wang M, Chen L, Zhang Z, Wang X, Qin S, Yan P.	AMB Express	10.1186/s13568-017-0361-x	2017
Metabolism	Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase.	Zhu B, Chen M, Yin H, Du Y, Ning L.	Mar Drugs	10.3390/md14060108	2016
Phylogeny	Variation in Sodic Soil Bacterial Communities Associated with Different Alkali Vegetation Types.	Borsodi AK, Mucsi M, Krett G, Szabo A, Felfoldi T, Szili-Kovacs T.	Microorganisms	10.3390/microorganisms9081673	2021
Metabolism	Characterization of a thermostable endoglucanase produced by Isoptericola variabilis sp. IDAH9.	Azizi M, Hemmat J, Seifati SM, Torktaz I, Karimi S.	Braz J Microbiol	10.1590/s1517-838246420140846	2015
Genetics	(Meta)genomic insights into the pathogenome of Cellulosimicrobium cellulans.	Sharma A, Gilbert JA, Lal R.	Sci Rep	10.1038/srep25527	2016
Enzymology	Thermophilic and alkaliphilic Actinobacteria: biology and potential applications.	Shivlata L, Satyanarayana T.	Front Microbiol	10.3389/fmicb.2015.01014	2015
	Isoptericola croceus sp. nov., a novel actinobacterium isolated from saline-alkali soil.	OuYang YT, Lv AP, Liu L, Li TT, Zhou Y, She TT, Cao LX, Jiao JY, Wang S, Li WJ.	Antonie Van Leeuwenhoek	10.1007/s10482-023-01849-6	2023
	Isoptericola rhizosphaerae sp. nov. and Isoptericola aurantiacus sp. nov. two novel bacteria isolated from rhizosphere soil.	Liu H, Yang Q, Li J, Yang F, Li C, Li D, Sun Y, Yang L, Jiang M.	Antonie Van Leeuwenhoek	10.1007/s10482-025-02105-9	2025
	Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study.	Vidal-Verdu A, Latorre-Perez A, Pascual J, Manes-Collado R, Nevot-Terraes A, Porcar M.	Front Microbiol	10.3389/fmicb.2025.1471121	2025
Phylogeny	Isoptericola sediminis sp. nov., Isolated from Chilika Lagoon.	Kumar D, U J, A K, G S, Sasikala C, Ramana CV	Curr Microbiol	10.1007/s00284-020-02325-4	2021
Phylogeny	Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China.	Zhang YQ, Schumann P, Li WJ, Chen GZ, Tian XP, Stackebrandt E, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.63641-0	2005

References

#6413	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16376
#19740	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Isoptericola halotolerans (DSM 16376, KCTC 19046, YIM 70177)

Name and taxonomic classification

Morphology

Cell morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Murein

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_013149785

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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