Strain identifier

BacDive ID: 12549

Type strain: Yes

Species: Prevotella pallens

Strain history: CIP <- 1998, E. Könönen, National Public Health Inst., Helsinki, Finland: strain AHN 10371

NCBI tax ID(s): 997353 (strain), 60133 (species)

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General

@ref: 7671

BacDive-ID: 12549

DSM-Number: 18710

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Prevotella pallens DSM 18710 is an anaerobe, mesophilic bacterium that was isolated from human saliva, young child.

NCBI tax id

NCBI tax idMatching level
997353strain
60133species

strain history

@refhistory
7671<- CIP <- E. Könönen
340541998, E. Könönen, Nat. Public Health Inst., Helsinki, Finland: strain AHN 10371
67770E. Könönen AHN 10371.
121509CIP <- 1998, E. Könönen, National Public Health Inst., Helsinki, Finland: strain AHN 10371

doi: 10.13145/bacdive12549.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Prevotella
  • species: Prevotella pallens
  • full scientific name: Prevotella pallens Könönen et al. 1998

@ref: 7671

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella pallens

full scientific name: Prevotella pallens Könönen et al. 1998 emend. Hahnke et al. 2016

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no90.92
6948099.999negative
121509nonegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7671COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
7671PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
34054MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
121509CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
7671positivegrowth37mesophilic
34054positivegrowth37mesophilic
54814positivegrowth37mesophilic
67770positivegrowth37mesophilic
121509positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
7671anaerobe
54814anaerobe
69480anaerobe99.464

spore formation

@refspore formationconfidence
69481no100
69480no99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase+
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
7671-+---+-----+---+++-+----+--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
7671human saliva, young childHelsinkiFinlandFINEurope
54814Human saliva,young childHelsinki ?FinlandFINEurope
67770Human oral cavity
121509Human, SalivaFinlandFINEurope1992

isolation source categories

Cat1Cat2Cat3
#Host#Human#Child
#Host Body Product#Oral cavity and Airways#Saliva

taxonmaps

  • @ref: 69479
  • File name: preview.99_6460.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_295;96_3058;97_3756;98_4767;99_6460&stattab=map
  • Last taxonomy: Prevotella pallens subclade
  • 16S sequence: Y13105
  • Sequence Identity:
  • Total samples: 66183
  • soil counts: 1018
  • aquatic counts: 1248
  • animal counts: 63428
  • plant counts: 489

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
76712Risk group (German classification)
1215092Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
7671Prevotella pallens strain 10371 16S ribosomal RNA, partial sequenceNR_0264171454nuccore60133
67770Prevotella pallens gene for 16S ribosomal RNA, partial sequence, strain: JCM 11140AB5477031492ena997353
67770Prevotella pallens 16S rRNA gene, strain 10371Y131051454ena997353

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella pallens ATCC 700821997353.4wgspatric997353
66792Prevotella pallens strain DSM 1871060133.3wgspatric60133
66792Prevotella pallens ATCC 700821651324090draftimg997353
66792Prevotella pallens DSM 187102695420923draftimg60133
67770Prevotella pallens ATCC 700821GCA_000220255scaffoldncbi997353
67770Prevotella pallens DSM 18710GCA_003269195scaffoldncbi60133

GC content

  • @ref: 7671
  • GC-content: 37.4
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.373no
flagellatedno96.965no
gram-positiveno97.92no
anaerobicyes98.858no
halophileno82.386no
spore-formingno96.863no
glucose-utilyes82.156no
aerobicno98.247yes
thermophileno96.276yes
glucose-fermentyes50.863no

External links

@ref: 7671

culture collection no.: DSM 18710, ATCC 700821, CCUG 39484, CIP 105551, NCTC 13042, AHN 10371, JCM 11140

straininfo link

  • @ref: 81764
  • straininfo: 44156

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9542075Phylogenetic characterization and proposal of a new pigmented species to the genus Prevotella: Prevotella pallens sp. nov.Kononen E, Eerola E, Frandsen EV, Jalava J, Matto J, Salmenlinna S, Jousimies-Somer HInt J Syst Bacteriol10.1099/00207713-48-1-471998Acetylglucosaminidase/metabolism, Carbohydrate Metabolism, Indoles/metabolism, Molecular Sequence Data, Phylogeny, Pigments, Biological/metabolism, Prevotella/*classification/enzymology/*genetics, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, alpha-L-Fucosidase/metabolismMetabolism
Phylogeny19196771Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque.Sakamoto M, Kumada H, Hamada N, Takahashi Y, Okamoto M, Bakir MA, Benno YInt J Syst Evol Microbiol10.1099/ijs.0.002626-02009Animals, Dental Plaque/*microbiology, Fatty Acids/analysis, Macaca fascicularis/*microbiology, Molecular Sequence Data, Phylogeny, Prevotella/chemistry/*classification/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Phylogeny19654360Prevotella aurantiaca sp. nov., isolated from the human oral cavity.Sakamoto M, Suzuki N, Okamoto MInt J Syst Evol Microbiol10.1099/ijs.0.012831-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Humans, Molecular Sequence Data, Mouth/*microbiology, Periodontitis/microbiology, Phylogeny, Prevotella/*classification/genetics/*isolation & purification/metabolism, RNA, Ribosomal, 16S/geneticsMetabolism

Reference

@idauthorscataloguedoi/urltitle
7671Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18710)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18710
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34054Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17583
54814Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39484)https://www.ccug.se/strain?id=39484
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81764Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44156.1StrainInfo: A central database for resolving microbial strain identifiers
121509Curators of the CIPCollection of Institut Pasteur (CIP 105551)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105551