Strain identifier
BacDive ID: 1249
Type strain: 
Species: Neobacillus pocheonensis
Strain history: <- ST Lee, KAIST
NCBI tax ID(s): 363869 (species)
version 9.1 (current version)
General
@ref: 7403
BacDive-ID: 1249
DSM-Number: 18135
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, rod-shaped
description: Neobacillus pocheonensis DSM 18135 is an aerobe, spore-forming, rod-shaped bacterium that was isolated from soil, ginseng field.
NCBI tax id
- NCBI tax id: 363869
- Matching level: species
strain history
| @ref | history |
|---|---|
| 7403 | <- Sung-Taik Lee <- Wan-Taek Im, Gsoil 420 |
| 67771 | <- ST Lee, KAIST |
doi: 10.13145/bacdive1249.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Neobacillus
- species: Neobacillus pocheonensis
- full scientific name: Neobacillus pocheonensis (Ten et al. 2007) Gupta et al. 2020
synonyms
- @ref: 20215
- synonym: Bacillus pocheonensis
@ref: 7403
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Neobacillus
species: Neobacillus pocheonensis
full scientific name: Neobacillus pocheonensis (Ten et al. 2007) Gupta et al. 2020
type strain: yes
Morphology
cell morphology
| @ref | cell shape | motility | gram stain |
|---|---|---|---|
| 67771 | rod-shaped | no | |
| 67771 | positive |
Culture and growth conditions
culture medium
- @ref: 7403
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 7403 | positive | growth | 30 |
| 67771 | positive | growth | 25 |
Physiology and metabolism
oxygen tolerance
- @ref: 67771
- oxygen tolerance: aerobe
spore formation
| @ref | type of spore | spore formation | confidence |
|---|---|---|---|
| 67771 | endospore | yes | |
| 69480 | yes | 92.391 |
observation
- @ref: 67771
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent | geographic location |
|---|---|---|---|---|---|
| 7403 | soil, ginseng field | Republic of Korea | KOR | Asia | |
| 67771 | From soil of the ginseng field | Republic of Korea | KOR | Asia | Pocheon Province |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Agriculture | #Field |
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
Safety information
risk assessment
- @ref: 7403
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7403
- description: Bacillus pocheonensis gene for 16S rRNA, partial sequence, strain: Gsoil 420
- accession: AB245377
- length: 1495
- database: nuccore
- NCBI tax ID: 363869
Genome sequences
- @ref: 66792
- description: Neobacillus pocheonensis KCTC 13943
- accession: GCA_023702235
- assembly level: contig
- database: ncbi
- NCBI tax ID: 363869
GC content
- @ref: 7403
- GC-content: 44.9
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 72.146 | yes |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 90.719 | yes |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 66.977 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 92.391 | yes |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 91.643 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 62.696 | yes |
External links
@ref: 7403
culture collection no.: DSM 18135, KCTC 13943, Gsoil 420
straininfo link
- @ref: 70905
- straininfo: 403948
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 17978214 | Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field. | Ten LN, Baek SH, Im WT, Larina LL, Lee JS, Oh HM, Lee ST | Int J Syst Evol Microbiol | 10.1099/ijs.0.64491-0 | 2007 | Aerobiosis, Bacillus/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Korea, Molecular Sequence Data, Panax/*growth & development, Phenotype, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*metabolism, *Soil Microbiology, Species Specificity | Genetics |
| Phylogeny | 22611202 | Bacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. | Nguyen NL, Kim YJ, Hoang VA, Min JW, Liang ZQ, Yang DC | Int J Syst Evol Microbiol | 10.1099/ijs.0.031740-0 | 2012 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Panax/*microbiology, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology | Genetics |
| Metabolism | 27084709 | Bacillus mesophilus sp. nov., an alginate-degrading bacterium isolated from a soil sample collected from an abandoned marine solar saltern. | Zhou YX, Liu GH, Liu B, Chen GJ, Du ZJ | Antonie Van Leeuwenhoek | 10.1007/s10482-016-0692-7 | 2016 | Alginates/*metabolism, Bacillus/genetics/*isolation & purification/*metabolism, Cell Wall/metabolism, China, Fatty Acids/metabolism, Genotype, Glucuronic Acid/metabolism, Hexuronic Acids/metabolism, Nucleic Acid Hybridization, Peptidoglycan/metabolism, Phenotype, Phosphatidylethanolamines/metabolism, Phylogeny, Sequence Analysis, DNA, Sodium Chloride, Soil/chemistry, *Soil Microbiology, Vitamin K 2/metabolism | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 7403 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18135) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18135 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 70905 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403948.1 | StrainInfo: A central database for resolving microbial strain identifiers |