Strain identifier

BacDive ID: 1243

Type strain: Yes

Species: Metabacillus niabensis

Strain Designation: 4T19

Strain history: CIP <- 2008, DSMZ <- B.Y. Kim, KACC, Suwon, Korea: strain 4T19

NCBI tax ID(s): 324854 (species)

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General

@ref: 6919

BacDive-ID: 1243

DSM-Number: 17723

keywords: genome sequence, 16S sequence, Bacteria, facultative aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Metabacillus niabensis 4T19 is a facultative aerobe, spore-forming, mesophilic bacterium that was isolated from compost for cultivation of mushroom.

NCBI tax id

  • NCBI tax id: 324854
  • Matching level: species

strain history

@refhistory
6919<- Byung-Yong Kim, 4T19
67770KACC 11279 <-- H.-Y. Weon 4T19.
121288CIP <- 2008, DSMZ <- B.Y. Kim, KACC, Suwon, Korea: strain 4T19

doi: 10.13145/bacdive1243.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Metabacillus
  • species: Metabacillus niabensis
  • full scientific name: Metabacillus niabensis (Kwon et al. 2007) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus niabensis

@ref: 6919

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Metabacillus

species: Metabacillus niabensis

full scientific name: Metabacillus niabensis (Kwon et al. 2007) Patel and Gupta 2020

strain designation: 4T19

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31749positive02-03 µm0.5-0.7 µmrod-shapedyes
121288positiverod-shapedyes

pigmentation

  • @ref: 31749
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6919CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
40071MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121288CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6919positivegrowth30mesophilic
31749positivegrowth04-45
31749positiveoptimum30mesophilic
40071positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
31749positivegrowth06-08
31749positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31749facultative aerobe
121288facultative anaerobe

spore formation

  • @ref: 31749
  • spore formation: yes

halophily

  • @ref: 31749
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

observation

@refobservation
31749aggregates in chains
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3174922599arabinose+carbon source
3174917234glucose+carbon source
3174929864mannitol+carbon source
3174918222xylose+carbon source
317494853esculin+hydrolysis
3174917632nitrate+reduction
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12128817632nitrate+reduction
12128816301nitrite-reduction

metabolite production

  • @ref: 121288
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31749catalase+1.11.1.6
31749gelatinase+
31749urease+3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121288oxidase+
121288catalase+1.11.1.6
121288urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121288-+++------++-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121288---+/-------+/-+/-+/--+/----------+--+-+/-+/-----++/------------+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6919compost for cultivation of mushroomSuwonRepublic of KoreaKORAsia
67770Cotton-waste composts for mushroom cultivationSuwonRepublic of KoreaKORAsia
121288Mushroom, cultivationSuwonRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Composting
#Engineered#Food production#Food
#Host#Fungi#Mushroom

taxonmaps

  • @ref: 69479
  • File name: preview.99_126.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_96;97_103;98_112;99_126&stattab=map
  • Last taxonomy: Metabacillus halosaccharovorans
  • 16S sequence: AY998119
  • Sequence Identity:
  • Total samples: 817
  • soil counts: 464
  • aquatic counts: 69
  • animal counts: 265
  • plant counts: 19

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
69191Risk group (German classification)
1212881Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6919
  • description: Bacillus niabensis strain 4T19 16S ribosomal RNA gene, partial sequence
  • accession: AY998119
  • length: 1478
  • database: ena
  • NCBI tax ID: 324854

Genome sequences

  • @ref: 66792
  • description: Bacillus niabensis DSM 17723
  • accession: 2926518972
  • assembly level: draft
  • database: img
  • NCBI tax ID: 324854

GC content

@refGC-contentmethod
691939.3
3174935.2
6777040.9high performance liquid chromatography (HPLC)

External links

@ref: 6919

culture collection no.: DSM 17723, CIP 109816, KACC 11279, JCM 16399

straininfo link

  • @ref: 70899
  • straininfo: 309433

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17684280Bacillus niabensis sp. nov., isolated from cotton-waste composts for mushroom cultivation.Kwon SW, Lee SY, Kim BY, Weon HY, Kim JB, Go SJ, Lee GBInt J Syst Evol Microbiol10.1099/ijs.0.64178-02007Agaricales, Bacillus/genetics/*isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Gossypium/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyGenetics
Phylogeny26362330A heavy metal tolerant novel bacterium, Bacillus malikii sp. nov., isolated from tannery effluent wastewater.Abbas S, Ahmed I, Kudo T, Iqbal M, Lee YJ, Fujiwara T, Ohkuma MAntonie Van Leeuwenhoek10.1007/s10482-015-0584-22015Aerobiosis, Bacillus/*classification/drug effects/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Drug Resistance, Bacterial, Fatty Acids/analysis, Glycolipids/analysis, Hydrogen-Ion Concentration, Industrial Waste, Locomotion, Metals, Heavy/*toxicity, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Waste Water/*microbiologyGenetics
Phylogeny26373875Bacillus endolithicus sp. nov., isolated from pebbles.Parag B, Sasikala C, Ramana CVInt J Syst Evol Microbiol10.1099/ijsem.0.0006122015Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial/genetics, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny26410293Bacillus cellulasensis sp. nov., isolated from marine sediment.Mawlankar R, Thorat MN, Krishnamurthi S, Dastager SGArch Microbiol10.1007/s00203-015-1155-72015Bacillus/*classification/genetics/*isolation & purification, Base Composition, Fatty Acids/analysis, Geologic Sediments/*microbiology, India, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Species SpecificityEnzymology
Phylogeny34150213Draft genome of Metabacillus niabensis strain 4T19(T) isolated from cotton-waste composts for mushroom cultivation.Kangale LJ, Levasseur A, Raoult D, Ghigo E, Fournier PENew Microbes New Infect10.1016/j.nmni.2021.1008942021Cultivation

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6919Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17723)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17723
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31749Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2802328776041
40071Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7601
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70899Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309433.1StrainInfo: A central database for resolving microbial strain identifiers
121288Curators of the CIPCollection of Institut Pasteur (CIP 109816)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109816