Name: Metabacillus niabensis 4T19
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1243

Type strain:

Species: Metabacillus niabensis

Strain Designation: 4T19

Culture col. no.: DSM 17723, CIP 109816, KACC 11279, JCM 16399

Strain history: CIP <- 2008, DSMZ <- B.Y. Kim, KACC, Suwon, Korea: strain 4T19

NCBI tax ID(s): 324854 (species)

SI-ID 309433

DSM 17723, KACC 11279, CIP 109816, JCM 16399

Special Collections

Section

Metabacillus niabensis 4T19 is a facultative aerobe, spore-forming, mesophilic bacterium that was isolated from compost for cultivation of mushroom.

Gram-positive
motile
rod-shaped
spore-forming
facultative aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Metabacillus*
	Species	**Metabacillus niabensis**
	Full Scientific Name (LPSN)	Metabacillus niabensis (Kwon et al. 2007) Patel and Gupta 2020

Synonym

Bacillus niabensis

Information on morphological and physiological properties Morphology

[Ref.: #31749]	Gram stain	positive
[Ref.: #121288]	Gram stain	positive

[Ref.: #31749]	Cell length	02-03 µm

[Ref.: #31749]	Cell width	0.5-0.7 µm

[Ref.: #31749]	Cell shape	rod-shaped
[Ref.: #121288]	Cell shape	rod-shaped

[Ref.: #31749]	Motility	yes
[Ref.: #121288]	Motility	yes

[Ref.: #31749]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6919]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #6919]

Culture medium growth

[Ref.: #6919]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6919]

Culture medium composition

expand / minimize

[Ref.: #40071]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40071]

Culture medium growth

[Ref.: #40071]

Culture medium composition

expand / minimize

[Ref.: #121288]

Culture medium

CIP Medium 72

[Ref.: #121288]

Culture medium growth

[Ref.: #121288]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6919]	growth	30 °C
[Ref.: #31749]	growth	04-45 °C
[Ref.: #31749]	optimum	30 °C
[Ref.: #40071]	growth	30 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #31749]			optimum	7
[Ref.: #31749]			growth	06-08

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31749]	Oxygen tolerance	facultative aerobe
[Ref.: #121288]	Oxygen tolerance	facultative anaerobe

[Ref.: #31749]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31749]		NaCl		growth	0-5	%

[Ref.: #31749]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31749]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31749]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31749]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31749]	mannitol ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31749]	nitrate ChEBI	+	reduction
[Ref.: #121288]	nitrate ChEBI	+	reduction
[Ref.: #121288]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31749]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121288]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31749]	catalase	+	1.11.1.6
[Ref.: #121288]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #31749]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121288]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #31749]	urease	+	3.5.1.5
[Ref.: #121288]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121288]	11662	not determinedn.d.	-	-	-	+/-	-	-	-	-	-	-	+/-	+/-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	+/-	+/-	-	-	-	-	+	+/-	-	-	-	-	-	-	-	-	-	-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121288]	12131	-	+	+	+	-	-	-	-	-	-	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6919]	Sample type/isolated from	compost for cultivation of mushroom
[Ref.: #6919]	Geographic location (country and/or sea, region)	Suwon
[Ref.: #6919]	Country	Republic of Korea
[Ref.: #6919]	Country ISO 3 Code	KOR
[Ref.: #6919]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Cotton-waste composts for mushroom cultivation
[Ref.: #67770]	Geographic location (country and/or sea, region)	Suwon
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #121288]	Sample type/isolated from	Mushroom, cultivation
[Ref.: #121288]	Geographic location (country and/or sea, region)	Suwon
[Ref.: #121288]	Country	Republic of Korea
[Ref.: #121288]	Country ISO 3 Code	KOR
[Ref.: #121288]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Engineered	#Food production	#Food
#Host	#Fungi	#Mushroom

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY998119 (>99% sequence identity) for Metabacillus halosaccharovorans from Microbeatlas

Aquatic Samples	69
Soil Samples	464
Animal Samples	265
Plant Samples	19
Total Samples	817

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6919]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121288]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6919]	Bacillus niabensis strain 4T19 16S ribosomal RNA gene, partial sequence	AY998119	1478	GenBank ENA	324854 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus niabensis DSM 17723	2926518972	draft	JGI IMG/M	324854 tax ID	*

[Ref.: #6919]	GC-content	39.3 mol%
[Ref.: #31749]	GC-content	35.2 mol%
[Ref.: #67770]	GC-content	40.9 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #6919]

Culture collection no.

DSM 17723, CIP 109816, KACC 11279, JCM 16399

[Ref.: #70899]

SI-ID 309433

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Draft genome of Metabacillus niabensis strain 4T19(T) isolated from cotton-waste composts for mushroom cultivation.	Kangale LJ, Levasseur A, Raoult D, Ghigo E, Fournier PE	New Microbes New Infect	10.1016/j.nmni.2021.100894	2021	*
Phylogeny	Bacillus cellulasensis sp. nov., isolated from marine sediment.	Mawlankar R, Thorat MN, Krishnamurthi S, Dastager SG	Arch Microbiol	10.1007/s00203-015-1155-7	2015	*
Phylogeny	Bacillus endolithicus sp. nov., isolated from pebbles.	Parag B, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijsem.0.000612	2015	*
Phylogeny	A heavy metal tolerant novel bacterium, Bacillus malikii sp. nov., isolated from tannery effluent wastewater.	Abbas S, Ahmed I, Kudo T, Iqbal M, Lee YJ, Fujiwara T, Ohkuma M	Antonie Van Leeuwenhoek	10.1007/s10482-015-0584-2	2015	*
Phylogeny	Bacillus niabensis sp. nov., isolated from cotton-waste composts for mushroom cultivation.	Kwon SW, Lee SY, Kim BY, Weon HY, Kim JB, Go SJ, Lee GB	Int J Syst Evol Microbiol	10.1099/ijs.0.64178-0	2007	*

References

#6919

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17723

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31749

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28023 (see below)

#40071 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70899

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID309433.1 )

#121288 Collection of Institut Pasteur ; Curators of the CIP; CIP 109816

#28023

IJSEM 1909 2007 ( DOI 10.1099/ijs.0.64178-0 , PubMed 17684280 )

* These data were automatically processed and therefore are not curated

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