Strain identifier

BacDive ID: 1208

Type strain: Yes

Species: Pseudalkalibacillus decolorationis

Strain Designation: mcha2343

Strain history: CIP <- 2004, DSMZ <- LMG <- J. Heyrman, Ghent Univ., Ghent, Belgium

NCBI tax ID(s): 163879 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5604

BacDive-ID: 1208

DSM-Number: 14890

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-variable, motile, rod-shaped, colony-forming

description: Pseudalkalibacillus decolorationis mcha2343 is an obligate aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from mural painting.

NCBI tax id

  • NCBI tax id: 163879
  • Matching level: species

strain history

@refhistory
5604<- D. Janssens, LMG; mcha2343 <- J. Heyrman, Univ. Ghent, Belgium
118861CIP <- 2004, DSMZ <- LMG <- J. Heyrman, Ghent Univ., Ghent, Belgium

doi: 10.13145/bacdive1208.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Pseudalkalibacillus
  • species: Pseudalkalibacillus decolorationis
  • full scientific name: Pseudalkalibacillus decolorationis (Heyrman et al. 2003) Joshi et al. 2022

  • synonyms

    @refsynonym
    20215Alkalihalobacillus decolorationis
    20215Bacillus decolorationis

@ref: 5604

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Pseudalkalibacillus

species: Pseudalkalibacillus decolorationis

full scientific name: Pseudalkalibacillus decolorationis (Heyrman et al. 2003) Joshi et al. 2022

strain designation: mcha2343

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23308variable1.0-4.0 µm0.5-0.8 µmrod-shapedyes
118861positiverod-shapedno

colony morphology

@refcolony colorcolony shapemedium used
23308cream-coloured to beigecircularTSA
118861

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5604HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)yeshttps://mediadive.dsmz.de/medium/428Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
23308Trypticase Soy Agar (TSA)yes
33233MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118861CIP Medium 116yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=116
118861CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5604positivegrowth28mesophilic
23308positivegrowth5-40
23308positiveoptimum25-37mesophilic
33233positivegrowth30mesophilic
118861positivegrowth22-30
118861nogrowth10psychrophilic
118861nogrowth37mesophilic
118861nogrowth45thermophilic
118861nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
23308inconsistentgrowth6.0
23308positivegrowth6.5
23308positivegrowth7.0
23308positivegrowth7.5
23308positivegrowth8.0
23308positivegrowth9.0alkaliphile
118861positivegrowth6

Physiology and metabolism

oxygen tolerance

  • @ref: 23308
  • oxygen tolerance: obligate aerobe

spore formation

@refspore descriptiontype of sporespore formation
23308ellipsoidal, nearly spherical, central to subterminal, swelled sporangia slightlyendosporeyes
118861yes

halophily

@refsaltgrowthtested relationconcentration
23308NaClpositivegrowth0-10 %
23308NaClpositiveoptimum4-7 %
23308NaClnogrowth12 %
23308NaClnogrowth15 %
118861NaClpositivegrowth2-10 %
118861NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2330816947citrate-assimilation
23308168082-dehydro-D-gluconate-builds acid from
2330827613amygdalin-builds acid from
2330815963ribitol-builds acid from
2330817108D-arabinose-builds acid from
2330818333D-arabitol-builds acid from
2330828847D-fucose-builds acid from
2330862318D-lyxose-builds acid from
233086731melezitose-builds acid from
2330828053melibiose-builds acid from
2330816634raffinose-builds acid from
2330817992sucrose-builds acid from
2330817924D-sorbitol-builds acid from
2330816443D-tagatose-builds acid from
2330832528turanose-builds acid from
2330865327D-xylose-builds acid from
2330816813galactitol-builds acid from
2330817113erythritol-builds acid from
2330824265gluconate-builds acid from
2330828087glycogen-builds acid from
2330817268myo-inositol-builds acid from
2330815443inulin-builds acid from
2330830849L-arabinose-builds acid from
2330818403L-arabitol-builds acid from
2330818287L-fucose-builds acid from
2330862345L-rhamnose-builds acid from
2330817266L-sorbose-builds acid from
2330865328L-xylose-builds acid from
23308320061methyl alpha-D-glucopyranoside-builds acid from
2330843943methyl alpha-D-mannoside-builds acid from
2330874863methyl beta-D-xylopyranoside-builds acid from
2330828017starch-builds acid from
2330817151xylitol-builds acid from
2330818305arbutin+builds acid from
2330816899D-mannitol+builds acid from
2330817754glycerol+builds acid from
23308casein+hydrolysis
233084853esculin+hydrolysis
233085291gelatin+hydrolysis
2330817632nitrate+reduction
23308174265-dehydro-D-gluconate+/-builds acid from
2330817057cellobiose+/-builds acid from
2330815824D-fructose+/-builds acid from
2330812936D-galactose+/-builds acid from
2330817634D-glucose+/-builds acid from
2330817716lactose+/-builds acid from
2330817306maltose+/-builds acid from
2330816024D-mannose+/-builds acid from
2330816988D-ribose+/-builds acid from
2330816551D-trehalose+/-builds acid from
2330828066gentiobiose+/-builds acid from
23308506227N-acetylglucosamine+/-builds acid from
2330817814salicin+/-builds acid from
11886116947citrate-carbon source
1188614853esculin-hydrolysis
118861606565hippurate-hydrolysis
11886117632nitrate-reduction
11886116301nitrite-reduction
11886117632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
2330835581indoleno
2330816136hydrogen sulfideno
2330815688acetoinno
11886135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2330815688acetoin-
11886115688acetoin-
11886117234glucose-

enzymes

@refvalueactivityec
23308arginine dihydrolase-3.5.3.6
23308beta-galactosidase+3.2.1.23
23308catalase+1.11.1.6
23308cytochrome oxidase+1.9.3.1
23308lysine decarboxylase-4.1.1.18
23308ornithine decarboxylase-4.1.1.17
23308tryptophan deaminase-4.1.99.1
23308urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
118861oxidase+
118861beta-galactosidase+3.2.1.23
118861alcohol dehydrogenase-1.1.1.1
118861gelatinase+/-
118861amylase-
118861DNase-
118861caseinase+3.4.21.50
118861catalase+1.11.1.6
118861tween esterase-
118861gamma-glutamyltransferase+2.3.2.2
118861lecithinase-
118861lipase-
118861lysine decarboxylase-4.1.1.18
118861ornithine decarboxylase-4.1.1.17
118861urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118861-+++------++-+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118861++-++-----------+-------------------------+-----------+------+--------+++-+---------+---+--------+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5604mural paintingHerbersteinAustriaAUTEurope
23308mural paintings, discoloured by microbial growths
118861Mural paintingAustriaAUTEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment
#Engineered#Other#Painting
#Host#Microbial

taxonmaps

  • @ref: 69479
  • File name: preview.99_103546.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_7922;97_9527;98_11698;99_103546&stattab=map
  • Last taxonomy: Alkalihalobacillus decolorationis
  • 16S sequence: AJ315075
  • Sequence Identity:
  • Total samples: 284
  • soil counts: 197
  • aquatic counts: 52
  • animal counts: 9
  • plant counts: 26

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56041Risk group (German classification)
1188611Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5604
  • description: Bacillus decolorationis 16S rRNA gene, type strain LMG 19507T
  • accession: AJ315075
  • length: 1503
  • database: ena
  • NCBI tax ID: 163879

Genome sequences

  • @ref: 66792
  • description: Pseudalkalibacillus decolorationis DSM 14890
  • accession: GCA_024609785
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 163879

GC content

@refGC-contentmethod
560439.8
2330839.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes73.09no
gram-positiveyes80.631yes
anaerobicno98.642no
aerobicyes89.499yes
halophileno54.895no
spore-formingyes95.282yes
motileyes88.791no
glucose-fermentno93.268no
thermophileno96.845no
glucose-utilyes91.531no

External links

@ref: 5604

culture collection no.: DSM 14890, CIP 108363, LMG 19507

straininfo link

  • @ref: 70864
  • straininfo: 13189

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12710613Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria).Heyrman J, Balcaen A, Rodriguez-Diaz M, Logan NA, Swings J, De Vos PInt J Syst Evol Microbiol10.1099/ijs.0.02452-02003Austria, Bacillus/*classification/genetics/isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, *Paintings, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/*analysis/genetics, SpainGenetics
Phylogeny22102083Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.Nedashkovskaya OI, Van Trappen S, Frolova GM, De Vos PArch Microbiol10.1007/s00203-011-0771-02011Animals, Bacillus/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, rRNA, Genotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Strongylocentrotus/*microbiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5604Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14890)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14890
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23308Jeroen Heyrman, An Balcaen, Marina Rodriguez-Diaz, Niall A. Logan, Jean Swings, Paul De Vos10.1099/ijs.0.02452-0Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria)IJSEM 53: 459-463 200312710613
33233Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5977
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70864Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13189.1StrainInfo: A central database for resolving microbial strain identifiers
118861Curators of the CIPCollection of Institut Pasteur (CIP 108363)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108363