Pseudalkalibacillus decolorationis (DSM 14890, CIP 108363, LMG 19507)

Name: Pseudalkalibacillus decolorationis (DSM 14890, CIP 108363, LMG 19507)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1208

Type strain

Strain Designation mcha2343

Culture col. no. DSM 14890 CIP 108363 LMG 19507 mcha2343

NCBI tax ID(s) 163879

Special Collections DSMZ CIP Literature strains

Links

Pseudalkalibacillus decolorationis mcha2343 is an obligate aerobe, spore-forming, mesophilic prokaryote that forms circular colonies and was isolated from mural painting.

spore-forming Gram-variable motile rod-shaped colony-forming obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 13189

LMG 19507,DSM 14890,KCTC 3850,CIP 108363

@ref 20215

Last LPSN update 2025-12-16 09:47:03

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Pseudalkalibacillus

Species Pseudalkalibacillus decolorationis

Full scientific name Pseudalkalibacillus decolorationis (Heyrman et al. 2003) Joshi et al. 2022

Synonyms (2)

Bacillus decolorationis
Alkalihalobacillus decolorationis

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
23308	variable	1.0-4.0 µm	0.5-0.8 µm	rod-shaped
118861	positive			rod-shaped

Colony morphology

@ref	Colony color	Colony shape	Medium used
23308	cream-coloured to beige	circular	TSA
118861

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5604	HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)	Medium recipe at MediaDive	Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
23308	Trypticase Soy Agar (TSA)
33233	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118861	CIP Medium 116	Medium recipe at CIP
118861	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5604	positive	growth	28	mesophilic
23308	positive	growth	5-40
23308	positive	optimum	25-37	mesophilic
33233	positive	growth	30	mesophilic
118861	positive	growth	22-30
118861	negative	growth	10
118861	negative	growth	37
118861	negative	growth	45
118861	negative	growth	55

Culture pH

@ref	Ability	Type	PH	PH range
23308	inconsistent	growth	6.0
23308	positive	growth	6.5
23308	positive	growth	7.0
23308	positive	growth	7.5
23308	positive	growth	8.0
23308	positive	growth	9.0	alkaliphile
118861	positive	growth	6

Physiology and metabolism

Oxygen tolerance

23308

Oxygen toleranceobligate aerobe

Spore formation

@ref	Spore description	Type of spore	Confidence
23308	ellipsoidal, nearly spherical, central to subterminal, swelled sporangia slightly	endospore
118861
125438			91.116

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23308	NaCl	positive	growth	0-10 %
23308	NaCl	positive	optimum	4-7 %
23308	NaCl		growth	12 %
23308	NaCl		growth	15 %
118861	NaCl	positive	growth	2-10 %
118861	NaCl		growth	0 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23308	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
23308	17426 ChEBI	5-dehydro-D-gluconate	+/-	builds acid from
23308	27613 ChEBI	amygdalin	-	builds acid from
23308	18305 ChEBI	arbutin	+	builds acid from
23308		casein	+	hydrolysis
23308	17057 ChEBI	cellobiose	+/-	builds acid from
23308	16947 ChEBI	citrate	-	assimilation
118861	16947 ChEBI	citrate	-	carbon source
23308	17108 ChEBI	D-arabinose	-	builds acid from
23308	18333 ChEBI	D-arabitol	-	builds acid from
23308	15824 ChEBI	D-fructose	+/-	builds acid from
23308	28847 ChEBI	D-fucose	-	builds acid from
23308	12936 ChEBI	D-galactose	+/-	builds acid from
23308	17634 ChEBI	D-glucose	+/-	builds acid from
23308	62318 ChEBI	D-lyxose	-	builds acid from
23308	16899 ChEBI	D-mannitol	+	builds acid from
23308	16024 ChEBI	D-mannose	+/-	builds acid from
23308	16988 ChEBI	D-ribose	+/-	builds acid from
23308	17924 ChEBI	D-sorbitol	-	builds acid from
23308	16443 ChEBI	D-tagatose	-	builds acid from
23308	16551 ChEBI	D-trehalose	+/-	builds acid from
23308	65327 ChEBI	D-xylose	-	builds acid from
23308	17113 ChEBI	erythritol	-	builds acid from
23308	4853 ChEBI	esculin	+	hydrolysis
118861	4853 ChEBI	esculin	-	hydrolysis
23308	16813 ChEBI	galactitol	-	builds acid from
23308	5291 ChEBI	gelatin	+	hydrolysis
23308	28066 ChEBI	gentiobiose	+/-	builds acid from
23308	24265 ChEBI	gluconate	-	builds acid from
23308	17754 ChEBI	glycerol	+	builds acid from
23308	28087 ChEBI	glycogen	-	builds acid from
118861	606565 ChEBI	hippurate	-	hydrolysis
23308	15443 ChEBI	inulin	-	builds acid from
23308	30849 ChEBI	L-arabinose	-	builds acid from
23308	18403 ChEBI	L-arabitol	-	builds acid from
23308	18287 ChEBI	L-fucose	-	builds acid from
23308	62345 ChEBI	L-rhamnose	-	builds acid from
23308	17266 ChEBI	L-sorbose	-	builds acid from
23308	65328 ChEBI	L-xylose	-	builds acid from
23308	17716 ChEBI	lactose	+/-	builds acid from
23308	17306 ChEBI	maltose	+/-	builds acid from
23308	6731 ChEBI	melezitose	-	builds acid from
23308	28053 ChEBI	melibiose	-	builds acid from
23308	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from
23308	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
23308	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
23308	17268 ChEBI	myo-inositol	-	builds acid from
23308	506227 ChEBI	N-acetylglucosamine	+/-	builds acid from
23308	17632 ChEBI	nitrate	+	reduction
118861	17632 ChEBI	nitrate	-	reduction
118861	17632 ChEBI	nitrate	-	respiration
118861	16301 ChEBI	nitrite	-	reduction
23308	16634 ChEBI	raffinose	-	builds acid from
23308	15963 ChEBI	ribitol	-	builds acid from
23308	17814 ChEBI	salicin	+/-	builds acid from
23308	28017 ChEBI	starch	-	builds acid from
23308	17992 ChEBI	sucrose	-	builds acid from
23308	32528 ChEBI	turanose	-	builds acid from
23308	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite
23308	15688 ChEBI	acetoin
23308	16136 ChEBI	hydrogen sulfide
23308	35581 ChEBI	indole
118861	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
23308	15688 ChEBI	acetoin	-
118861	15688 ChEBI	acetoin	-
118861	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
118861	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
118861	amylase	-
23308	arginine dihydrolase	-	3.5.3.6
23308	beta-galactosidase	+	3.2.1.23
68382	beta-galactosidase	+	3.2.1.23	from API zym
118861	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118861	caseinase	+	3.4.21.50
23308	catalase	+	1.11.1.6
118861	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
23308	cytochrome oxidase	+	1.9.3.1
118861	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
118861	gamma-glutamyltransferase	+	2.3.2.2
118861	gelatinase	+/-
118861	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
118861	lipase	-
68382	lipase (C 14)	-		from API zym
23308	lysine decarboxylase	-	4.1.1.18
118861	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
23308	ornithine decarboxylase	-	4.1.1.17
118861	ornithine decarboxylase	-	4.1.1.17
118861	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
23308	tryptophan deaminase	-	4.1.99.1
118861	tween esterase	-
23308	urease	-	3.5.1.5
118861	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118861	-	+	+	+	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
118861	not determinedn.d.	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	+	+	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	-
#Engineered	#Other	#Painting
#Host	#Microbial	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5604	mural painting	Herberstein	Austria	AUT	Europe
23308	mural paintings, discoloured by microbial growths
118861	Mural painting		Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ315075 (>99% sequence identity) for Alkalihalobacillus decolorationis from Microbeatlas

Aquatic Samples	52
Soil Samples	197
Animal Samples	9
Plant Samples	26
Total Samples	284

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5604	1	Risk group (German classification) According to TRBA 466
118861	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM2460978v1 assembly for Pseudalkalibacillus decolorationis DSM 14890	scaffold	GCA_024609785	163879.3	163879	53.38

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5604	Bacillus decolorationis 16S rRNA gene, type strain LMG 19507T	AJ315075	1503		163879

GC content

@ref	GC-content (mol%)	Method
5604	39.8
23308	39.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudalkalibacillus decolorationis DSM 14890
NCBI: GCA_024609785.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	78.11	no
125438	anaerobic	anaerobicⓘ	no	98.23	yes
125438	aerobic	aerobicⓘ	yes	76.99	yes
125438	spore-forming	spore-formingⓘ	yes	91.12	yes
125438	thermophilic	thermophileⓘ	no	88.42	yes
125438	flagellated	motile2+ⓘ	yes	81.35	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Alkalihalobacterium elongatum gen. nov. sp. nov.: An Antibiotic-Producing Bacterium Isolated From Lonar Lake and Reclassification of the Genus Alkalihalobacillus Into Seven Novel Genera.	Joshi A, Thite S, Karodi P, Joseph N, Lodha T.	Front Microbiol	10.3389/fmicb.2021.722369	2021
Phylogeny	Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.	Nedashkovskaya OI, Van Trappen S, Frolova GM, De Vos P	Arch Microbiol	10.1007/s00203-011-0771-0	2011
Phylogeny	Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria).	Heyrman J, Balcaen A, Rodriguez-Diaz M, Logan NA, Swings J, De Vos P	Int J Syst Evol Microbiol	10.1099/ijs.0.02452-0	2003

References

#5604	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14890
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23308	Jeroen Heyrman, An Balcaen, Marina Rodriguez-Diaz, Niall A. Logan, Jean Swings, Paul De Vos: Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria). IJSEM 53: 459 - 463 2003 ( DOI 10.1099/ijs.0.02452-0 , PubMed 12710613 )
#33233	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118861	Collection of Institut Pasteur ; Curators of the CIP; CIP 108363
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudalkalibacillus decolorationis (DSM 14890, CIP 108363, LMG 19507)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_024609785

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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