Name: Pseudalkalibacillus decolorationis mcha2343
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1208

Type strain:

Species: Pseudalkalibacillus decolorationis

Strain Designation: mcha2343

Culture col. no.: DSM 14890, CIP 108363, LMG 19507

Strain history: <- D. Janssens, LMG; mcha2343 <- J. Heyrman, Univ. Ghent, Belgium

NCBI tax ID(s): 163879 (species)

Section

Pseudalkalibacillus decolorationis mcha2343 is an obligate aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from mural painting.

colony-forming
Gram-variable
motile
rod-shaped
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Pseudalkalibacillus*
	Species	**Pseudalkalibacillus decolorationis**
	Full Scientific Name (PNU)	Pseudalkalibacillus decolorationis (Heyrman et al. 2003) Joshi et al. 2022

	Synonym	Alkalihalobacillus decolorationis
	Synonym	Bacillus decolorationis

Information on morphological and physiological properties Morphology

[Ref.: #23308]	Gram stain	variable
[Ref.: #23308]	Cell length	1.0-4.0 µm
[Ref.: #23308]	Cell width	0.5-0.8 µm
[Ref.: #23308]	Cell shape	rod-shaped
[Ref.: #23308]	Motility	yes

[Ref.: #23308]	Colony color	cream-coloured to beige
[Ref.: #23308]	Colony shape	circular
[Ref.: #23308]	Cultivation medium used	TSA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5604]

Culture medium

HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)

[Ref.: #5604]

Culture medium growth

[Ref.: #5604]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #5604]

Culture medium composition

expand / minimize

[Ref.: #23308]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #23308]

Culture medium growth

[Ref.: #33233]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #33233]

Culture medium growth

[Ref.: #33233]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5604]	growth	28 °C
[Ref.: #23308]	growth	5-40 °C
[Ref.: #23308]	optimum	25-37 °C
[Ref.: #33233]	growth	30 °C

[Ref.: #5604]	Temperature range	mesophilic
[Ref.: #23308]	Temperature range	mesophilic
[Ref.: #33233]	Temperature range	mesophilic

	Kind of pH		pH	pH range
[Ref.: #23308]	inconsistent	growth	6.0
[Ref.: #23308]		growth	6.5
[Ref.: #23308]		growth	7.0
[Ref.: #23308]		growth	7.5
[Ref.: #23308]		growth	8.0
[Ref.: #23308]		growth	9.0	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23308]

Oxygen tolerance

obligate aerobe

[Ref.: #23308]	Spore description	ellipsoidal, nearly spherical, central to subterminal, swelled sporangia slightly
[Ref.: #23308]	Type of spore	endospore
[Ref.: #23308]	Ability of spore formation	yes

	Salt	Tested relation		Salt conc.
[Ref.: #23308]	NaCl		growth	0-10	%
[Ref.: #23308]	NaCl		optimum	4-7	%
[Ref.: #23308]	NaCl	no	growth	12	%
[Ref.: #23308]	NaCl	no	growth	15	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23308]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23308]	5-dehydro-D-gluconate ChEBI	+/-	builds acid from
[Ref.: #23308]	amygdalin ChEBI	-	builds acid from
[Ref.: #23308]	arbutin ChEBI	+	builds acid from
[Ref.: #23308]	casein	+	hydrolysis
[Ref.: #23308]	cellobiose ChEBI	+/-	builds acid from
[Ref.: #23308]	citrate ChEBI	-	assimilation
[Ref.: #23308]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23308]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23308]	D-fructose ChEBI	+/-	builds acid from
[Ref.: #23308]	D-fucose ChEBI	-	builds acid from
[Ref.: #23308]	D-galactose ChEBI	+/-	builds acid from
[Ref.: #23308]	D-glucose ChEBI	+/-	builds acid from
[Ref.: #23308]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23308]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23308]	D-mannose ChEBI	+/-	builds acid from
[Ref.: #23308]	D-ribose ChEBI	+/-	builds acid from
[Ref.: #23308]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23308]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23308]	D-trehalose ChEBI	+/-	builds acid from
[Ref.: #23308]	D-xylose ChEBI	-	builds acid from
[Ref.: #23308]	erythritol ChEBI	-	builds acid from
[Ref.: #23308]	esculin ChEBI	+	hydrolysis
[Ref.: #23308]	galactitol ChEBI	-	builds acid from
[Ref.: #23308]	gelatin ChEBI	+	hydrolysis
[Ref.: #23308]	gentiobiose ChEBI	+/-	builds acid from
[Ref.: #23308]	gluconate ChEBI	-	builds acid from
[Ref.: #23308]	glycerol ChEBI	+	builds acid from
[Ref.: #23308]	glycogen ChEBI	-	builds acid from
[Ref.: #23308]	inulin ChEBI	-	builds acid from
[Ref.: #23308]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23308]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23308]	L-fucose ChEBI	-	builds acid from
[Ref.: #23308]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23308]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23308]	L-xylose ChEBI	-	builds acid from
[Ref.: #23308]	lactose ChEBI	+/-	builds acid from
[Ref.: #23308]	maltose ChEBI	+/-	builds acid from
[Ref.: #23308]	melezitose ChEBI	-	builds acid from
[Ref.: #23308]	melibiose ChEBI	-	builds acid from
[Ref.: #23308]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #23308]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23308]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #23308]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23308]	N-acetylglucosamine ChEBI	+/-	builds acid from
[Ref.: #23308]	nitrate ChEBI	+	reduction
[Ref.: #23308]	raffinose ChEBI	-	builds acid from
[Ref.: #23308]	ribitol ChEBI	-	builds acid from
[Ref.: #23308]	salicin ChEBI	+/-	builds acid from
[Ref.: #23308]	starch ChEBI	-	builds acid from
[Ref.: #23308]	sucrose ChEBI	-	builds acid from
[Ref.: #23308]	turanose ChEBI	-	builds acid from
[Ref.: #23308]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #23308]		acetoin ChEBI
[Ref.: #23308]		hydrogen sulfide ChEBI
[Ref.: #23308]		indole ChEBI

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #23308]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #23308]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23308]	beta-galactosidase	+	3.2.1.23
[Ref.: #23308]	catalase	+	1.11.1.6
[Ref.: #23308]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23308]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23308]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23308]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23308]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5604]	Sample type/isolated from	mural painting
[Ref.: #5604]	Geographic location (country and/or sea, region)	Herberstein
[Ref.: #5604]	Country	Austria
[Ref.: #5604]	Country ISO 3 Code	AUT
[Ref.: #5604]	Continent	Europe

[Ref.: #23308]	Sample type/isolated from	mural paintings, discoloured by microbial growths
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	-
#Engineered	#Other	#Painting
#Host	#Microbial	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ315075 (>99% sequence identity) for Alkalihalobacillus decolorationis from Microbeatlas

Aquatic Samples	52
Soil Samples	197
Animal Samples	9
Plant Samples	26
Total Samples	284

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5604]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5604]	Bacillus decolorationis 16S rRNA gene, type strain LMG 19507T	AJ315075	1503	ENA	163879 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudalkalibacillus decolorationis DSM 14890	GCA_024609785	scaffold	GenBank	163879 tax ID	*
[Ref.: #66792]	Pseudomonas aeruginosa 1608	GCA_003863605	contig	PATRIC	287 tax ID	*

[Ref.: #5604]	GC-content	39.8 mol%
[Ref.: #23308]	GC-content	39.8 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #5604]

Culture collection no.

DSM 14890, CIP 108363, LMG 19507

[Ref.: #20218]

Associated Passport(s) in StrainInfo

276961, 534243, 49534

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria).	Heyrman J, Balcaen A, Rodriguez-Diaz M, Logan NA, Swings J, De Vos P	Int J Syst Evol Microbiol	10.1099/ijs.0.02452-0	2003	*
Phylogeny	Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.	Nedashkovskaya OI, Van Trappen S, Frolova GM, De Vos P	Arch Microbiol	10.1007/s00203-011-0771-0	2011	*

References

#5604

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14890

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23308

Jeroen Heyrman, An Balcaen, Marina Rodriguez-Diaz, Niall A. Logan, Jean Swings, Paul De Vos: Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria). IJSEM 53: 459 - 463 2003 ( DOI 10.1099/ijs.0.02452-0 , PubMed 12710613 )

#33233 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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