Strain identifier

BacDive ID: 11903

Type strain: Yes

Species: Methylophaga marina

Strain Designation: 222

Strain history: CIP <- 1995, M. Janvier and F. Gasser, Inst. Pasteur, Paris, France: strain 222

NCBI tax ID(s): 1122235 (strain), 45495 (species)

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General

@ref: 2286

BacDive-ID: 11903

DSM-Number: 5689

keywords: 16S sequence, Bacteria, mesophilic

description: Methylophaga marina 222 is a mesophilic bacterium that was isolated from marine muds.

NCBI tax id

NCBI tax idMatching level
1122235strain
45495species

strain history

@refhistory
2286<- NCIMB <- F. Gasser, 222
356931995, M. Janvier and F. Gasser, Inst. Pasteur, Paris, France: strain 222
67770T. Urakami TK 1101 <-- NCMB 2244 <-- F. Gasser 222.
120799CIP <- 1995, M. Janvier and F. Gasser, Inst. Pasteur, Paris, France: strain 222

doi: 10.13145/bacdive11903.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Beggiatoales
  • family: Piscirickettsiaceae
  • genus: Methylophaga
  • species: Methylophaga marina
  • full scientific name: Methylophaga marina Janvier et al. 1985

@ref: 2286

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Thiotrichales

family: Piscirickettsiaceae

genus: Methylophaga

species: Methylophaga marina

full scientific name: Methylophaga marina Janvier et al. 1985

strain designation: 222

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2286MEDIUM FOR MARINE METHYLOTROPHS (DSMZ Medium 750)yeshttps://mediadive.dsmz.de/medium/750Name: MEDIUM FOR MARINE METHYLOTROPHS (DSMZ Medium 750; with strain-specific modifications) Composition: NaCl 25.0 g/l Agar 20.0 g/l Peptone 10.0 g/l None 10.0 g/l Methanol 7.92 g/l Beef extract 7.0 g/l (NH4)2SO4 1.0 g/l K2HPO4 1.0 g/l Distilled water
35693MEDIUM 138 - for Methylophaga thalassica and Methylophaga marinayesDistilled water make up to (972.000 ml);Sodium chloride (24.000 g);Potassium chloride (0.500 g);Magnesium chloride hexahydrate (3.000 g);Magnesium sulphate heptahydrate (2.000 g);Calcium chloride dihydrate (1.000 g);Methanol (6.000 ml);Trizma hydrochlorid
120799CIP Medium 138yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=138

culture temp

@refgrowthtypetemperaturerange
2286positivegrowth28mesophilic
35693positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: Q-8

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
2286marine muds
67770Marine mud
120799Environment, Marine mudEtel river estuaryFranceFRAEurope1976

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Mud (Sludge)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
22861Risk group (German classification)
1207991Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.marina gene for 16S ribosomal RNA (ATCC 35842)X873381488ena45495
20218M.marina mRNA for 16S ribosomal RNAX954591490ena45495
20218Methylophaga marina gene for 16S rRNA, partial sequence, strain: NBRC 102421AB6817771461ena45495

GC content

@refGC-contentmethod
6777044.5high performance liquid chromatography (HPLC)
6777043thermal denaturation, midpoint method (Tm)

External links

@ref: 2286

culture collection no.: DSM 5689, ATCC 35842, NCIMB 2244, JCM 6886, CIP 104551, IAM 14932, KCTC 2857, NBRC 102421, VKM B-2056, LMG 6788

straininfo link

  • @ref: 81118
  • straininfo: 3569

literature

  • topic: Phylogeny
  • Pubmed-ID: 17410879
  • title: [Vitamin B12-independent strains of Methylophaga marina isolated from Red Sea algae].
  • authors: Li TsD, Doronina NV, Ivanova EG, Trotsenko IuA
  • journal: Mikrobiologiia
  • year: 2007
  • mesh: Egypt, Eukaryota/*microbiology, Gammaproteobacteria/classification/*physiology, Genes, Bacterial, Gram-Negative Aerobic Bacteria/classification/physiology, *Marine Biology, Methanol/metabolism, Methylamines/metabolism, Oceans and Seas, Phylogeny, Species Specificity, Vitamin B 12/*metabolism, *Water Microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2286Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5689)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5689
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35693Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16472
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
81118Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3569.1StrainInfo: A central database for resolving microbial strain identifiers
120799Curators of the CIPCollection of Institut Pasteur (CIP 104551)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104551