Strain identifier

BacDive ID: 11886

Type strain: Yes

Species: Mesorhizobium huakuii

Strain Designation: 103

Strain history: CIP <- 2002, P. de Lajudie, Montpellier, France: strain ORS 1752T <- 1993, LMG <- IAM, Rhizobium huakuii <- W. Chen: strain 103 <- Nanjing Agriculture Univ., China

NCBI tax ID(s): 28104 (species)

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General

@ref: 2734

BacDive-ID: 11886

DSM-Number: 6573

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Mesorhizobium huakuii 103 is a mesophilic, Gram-negative, motile bacterium that was isolated from root nodules of Astragalus sinicus.

NCBI tax id

  • NCBI tax id: 28104
  • Matching level: species

strain history

@refhistory
2734<- W.X. Chen, 103
67770IAM 14158 <-- W. X. Chen CCBAU 2609.
119978CIP <- 2002, P. de Lajudie, Montpellier, France: strain ORS 1752T <- 1993, LMG <- IAM, Rhizobium huakuii <- W. Chen: strain 103 <- Nanjing Agriculture Univ., China

doi: 10.13145/bacdive11886.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium huakuii
  • full scientific name: Mesorhizobium huakuii (Chen et al. 1991) Jarvis et al. 1997

  • synonyms

    • @ref: 20215
    • synonym: Rhizobium huakuii

@ref: 2734

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium huakuii

full scientific name: Mesorhizobium huakuii (Chen et al. 1991) Jarvis et al. 1997

strain designation: 103

type strain: yes

Morphology

cell morphology

  • @ref: 119978
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refincubation period
27341-2 days
119978

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2734RHIZOBIUM MEDIUM (DSMZ Medium 98)yeshttps://mediadive.dsmz.de/medium/98Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
33770MEDIUM 296 - for RhizobiumyesDistilled water make up to (800.000 ml);Agar (15.000 g);Yeast extract (1.000 g);Mannitol (10.000 g);Earth extract - M0541 (200.000 ml)
119978CIP Medium 296yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=296

culture temp

@refgrowthtypetemperaturerange
2734positivegrowth30mesophilic
33770positivegrowth30mesophilic
67770positivegrowth25mesophilic
119978positivegrowth25-37mesophilic
119978nogrowth10psychrophilic
119978nogrowth41thermophilic
119978nogrowth45thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
119978NaClpositivegrowth0 %
119978NaClnogrowth2 %
119978NaClnogrowth4 %
119978NaClnogrowth6 %
119978NaClnogrowth8 %
119978NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate-assimilation24265
68369maltose+assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose+assimilation30849
68369D-glucose+assimilation17634
68369gelatin-hydrolysis5291
68369esculin+hydrolysis4853
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan-energy source27897
68369nitrate-reduction17632
119978citrate+carbon source16947
119978esculin+hydrolysis4853
119978hippurate-hydrolysis606565
119978nitrate-reduction17632
119978nitrite-reduction16301
119978nitrate-respiration17632

antibiotic resistance

  • @ref: 119978
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11997835581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11997815688acetoin-
11997817234glucose-

enzymes

@refvalueactivityec
2734catalase+1.11.1.6
2734cytochrome-c oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369arginine dihydrolase-3.5.3.6
119978oxidase+
119978beta-galactosidase-3.2.1.23
119978alcohol dehydrogenase-1.1.1.1
119978gelatinase-
119978amylase-
119978DNase-
119978caseinase-3.4.21.50
119978catalase-1.11.1.6
119978tween esterase-
119978gamma-glutamyltransferase+2.3.2.2
119978lecithinase-
119978lipase-
119978lysine decarboxylase-4.1.1.18
119978ornithine decarboxylase-4.1.1.17
119978phenylalanine ammonia-lyase-4.3.1.24
119978tryptophan deaminase-
119978urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119978-+++-+--+-++---+----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2734----++-+++++++---+--+
2734----+/-+-+++++++---+--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119978------------------------+/-------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119978+++++--+--++++++++++++-++-++++--++++++-+--+------+---------+--+--+-----+---+---++-----+---++-+-----

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentgeographic location
2734root nodules of Astragalus sinicusAstragalus sinicusChinaCHNAsia
67770Root nodules of Astragalus sinicusAstragalus sinicusChinaCHNAsiaNanjing
119978Astragalus sinicusChinaCHNAsiaNanjing

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_315.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_268;99_315&stattab=map
  • Last taxonomy: Mesorhizobium
  • 16S sequence: NR_116706
  • Sequence Identity:
  • Total samples: 49047
  • soil counts: 28701
  • aquatic counts: 8865
  • animal counts: 7451
  • plant counts: 4030

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
27341Risk group (German classification)
1199781Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mesorhizobium huakuii strain CCBAU 2609 16S ribosomal RNA gene, partial sequenceFJ4912641317ena28104
2021816S rRNA [Rhizobium huakuii, CCBAU2609T, Genomic, 260 nt]S74905260ena28104
20218Mesorhizobium huakuii gene for 16S rRNA, strain: IAM 14158D127971438ena28104
20218Mesorhizobium huakuii gene for 16S rRNA, complete sequence, type strain: IFO 15243D134311443ena28104
20218Mesorhizobium huakuii strain LMG 14107 16S ribosomal RNA gene, partial sequence; internal transcribed spacer, complete sequence; and 23S ribosomal RNA, partial sequenceAF345259857ena28104
20218Mesorhizobium huakuii gene for 16S rRNA, partial sequence, strain: NBRC 15243AB6808161410ena28104
20218Mesorhizobium huakuii strain USDA 4779 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceHQ143397930ena28104
2734Mesorhizobium huakuii strain CCBAU 2609 16S ribosomal RNA, partial sequenceNR_1167061317nuccore28104

External links

@ref: 2734

culture collection no.: DSM 6573, ATCC 51122, CCBAU 2609, IAM 14158, JCM 21036, HAMBI 1674, HAMBI 2035, IFO 15243, LMG 14107, NBRC 15243, OUT 30007, CIP 107328, ORS 1752T

straininfo link

  • @ref: 81103
  • straininfo: 11556

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics11117330Structure of the O-specific polysaccharide of Mesorhizobium huakuii IFO15243T.Choma A, Sowinski P, Mayer HCarbohydr Res10.1016/s0008-6215(00)00180-42000Carbohydrate Sequence, Gas Chromatography-Mass Spectrometry, Hydrolysis, Magnetic Resonance Spectroscopy, Methylation, Molecular Sequence Data, O Antigens/*chemistry/isolation & purification, Rhizobiaceae/*chemistryEnzymology
Phylogeny12545212Lipopolysaccharides from Mesorhizobium huakuii and Mesorhizobium ciceri: chemical and immunological comparative data.Choma AActa Biochim Pol02490410432002Alphaproteobacteria/*chemistry/*classification/immunology, Animals, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Immune Sera/immunology, Lipopolysaccharides/*chemistry/*immunology, Rabbits, Species SpecificityEnzymology
Metabolism15030481Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues.Choma A, Sowinski PEur J Biochem10.1111/j.1432-1033.2004.04038.x2004Alphaproteobacteria/*metabolism, Gas Chromatography-Mass Spectrometry, Glucosamine/*analogs & derivatives/chemistry, Hexuronic Acids/*chemistry, Hydrocarbons/chemistry, Lipid A/*chemistry, Lipids/chemistry, Magnetic Resonance Spectroscopy, Models, Chemical, Phosphates/*chemistry, Phosphorylation, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
20692653Synthesis of the 6-deoxytalose-containing tri- and hexasaccharides of the O-antigen polysaccharide from Mesorhizobium huakuii IFO15243T.Zong G, Feng Y, Liang X, Chen L, Zhang J, Wang DCarbohydr Res10.1016/j.carres.2010.07.0232010Deoxy Sugars/*chemistry, Hexoses/*chemistry, O Antigens/*chemistry, Oligosaccharides/*chemical synthesis, Rhizobiaceae/chemistry
Phylogeny25736411Revision of the taxonomic status of type strains of Mesorhizobium loti and reclassification of strain USDA 3471T as the type strain of Mesorhizobiumerdmanii sp. nov. and ATCC 33669T as the type strain of Mesorhizobiumjarvisii sp. nov.Martinez-Hidalgo P, Ramirez-Bahena MH, Flores-Felix JD, Rivas R, Igual JM, Mateos PF, Martinez-Molina E, Leon-Barrios M, Peix A, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.0001642015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Lotus/*microbiology, Mesorhizobium/*classification/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/microbiology, Sequence Analysis, DNA, United States, United States Department of AgricultureGenetics
Phylogeny26296780Mesorhizobium waimense sp. nov. isolated from Sophora longicarinata root nodules and Mesorhizobium cantuariense sp. nov. isolated from Sophora microphylla root nodules.De Meyer SE, Wee Tan H, Heenan PB, Andrews M, Willems AInt J Syst Evol Microbiol10.1099/ijsem.0.0004302015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, New Zealand, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Sophora/*microbiologyGenetics
Metabolism27253495Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance.Komaniecka I, Zamlynska K, Zan R, Staszczak M, Pawelec J, Seta I, Choma AActa Biochim Pol10.18388/abp.2015_12122016Anti-Bacterial Agents/metabolism/*pharmacology, Cell Membrane/metabolism, Cell Membrane Permeability, Drug Resistance, Bacterial, Microbial Sensitivity Tests, Microbial Viability, Polymyxin B/metabolism/*pharmacology, Rhizobium/drug effects/*physiology/ultrastructurePathogenicity
Phylogeny30457516Mesorhizobium carmichaelinearum sp. nov., isolated from Carmichaelineae spp. root nodules.De Meyer SE, Andrews M, James EK, Willems AInt J Syst Evol Microbiol10.1099/ijsem.0.0031202018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fabaceae/*microbiology, Fatty Acids/chemistry, Genes, Bacterial, Mesorhizobium/*classification/isolation & purification, New Zealand, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNATranscriptome
33391311Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties.Liu F, Yi M, Liu X, Shen Y, Li J, Wang H, Yang D, Sun ZFront Plant Sci10.3389/fpls.2020.5994002020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2734Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6573)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6573
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33770Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4752
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81103Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID11556.1StrainInfo: A central database for resolving microbial strain identifiers
119978Curators of the CIPCollection of Institut Pasteur (CIP 107328)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107328