Strain identifier
BacDive ID: 11831
Type strain: 
Species: Desulfotruncus alcoholivorax
Strain Designation: RE35E1
Strain history: DSM 16058 <-- A. Kaksonen RE35E1.
NCBI tax ID(s): 265477 (species)
version 8.1 (current version)
General
@ref: 6247
BacDive-ID: 11831
DSM-Number: 16058
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped
description: Desulfotruncus alcoholivorax RE35E1 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from fluidized-bed reactor.
NCBI tax id
- NCBI tax id: 265477
- Matching level: species
strain history
| @ref | history |
|---|---|
| 6247 | <- A. Kaksonen; RE35E1 |
| 67770 | DSM 16058 <-- A. Kaksonen RE35E1. |
doi: 10.13145/bacdive11831.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Peptococcaceae
- genus: Desulfotruncus
- species: Desulfotruncus alcoholivorax
- full scientific name: Desulfotruncus alcoholivorax (Kaksonen et al. 2008) Watanabe et al. 2021
synonyms
@ref synonym 20215 Desulfotomaculum alcoholivorax 20215 Desulfallas alcoholivorax
@ref: 6247
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Peptococcaceae
genus: Desulfallas
species: Desulfallas alcoholivorax
full scientific name: Desulfallas alcoholivorax (Kaksonen et al. 2008) Watanabe et al. 2018
strain designation: RE35E1
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 32137 | positive | 5 µm | 0.9 µm | rod-shaped | yes | |
| 69480 | yes | 94.386 | ||||
| 69480 | positive | 100 |
Culture and growth conditions
culture medium
- @ref: 6247
- name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)
- growth: yes
- link: https://mediadive.dsmz.de/medium/641
- composition: Name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641; with strain-specific modifications) Composition: Na-pyruvate 2.49252 g/l Na2SO4 1.99402 g/l Na2S2O3 x 5 H2O 0.997009 g/l MgSO4 x 7 H2O 0.997009 g/l NH4Cl 0.997009 g/l Yeast extract 0.997009 g/l Na2CO3 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.0997009 g/l CaCl2 x 2 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 6247 | positive | growth | 45 | thermophilic | |
| 32137 | positive | growth | 33-51 | ||
| 32137 | positive | optimum | 45 | thermophilic | |
| 67770 | positive | growth | 50 | thermophilic | |
| 69480 | thermophilic | 99.999 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 32137 | positive | growth | 6-7.5 |
| 32137 | positive | optimum | 6.85 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 6247 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 32137 | yes | |
| 69481 | yes | 100 |
| 69480 | yes | 100 |
halophily
- @ref: 32137
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-0.25 %
observation
- @ref: 67770
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 6247 | fluidized-bed reactor | Finland | FIN | Europe |
| 67770 | Fluidized-bed-reactor treating acidic water containing metal and sulfate |
isolation source categories
- Cat1: #Engineered
- Cat2: #Bioreactor
taxonmaps
- @ref: 69479
- File name: preview.99_88752.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_377;96_9239;97_46758;98_61277;99_88752&stattab=map
- Last taxonomy: Desulfallas alcoholivorax subclade
- 16S sequence: AY548778
- Sequence Identity:
- Total samples: 7
- soil counts: 6
- aquatic counts: 1
Safety information
risk assessment
- @ref: 6247
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6247
- description: Desulfotomaculum alcoholivorax strain RE35E1 16S ribosomal RNA gene gene, partial sequence
- accession: AY548778
- length: 1429
- database: ena
- NCBI tax ID: 1121422
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Desulfotomaculum alcoholivorax DSM 16058 | 1121422.3 | wgs | patric | 1121422 |
| 66792 | Desulfotomaculum alcoholivorax DSM 16058 | 2524023147 | draft | img | 1121422 |
| 67770 | Desulfotruncus alcoholivorax DSM 16058 | GCA_000430885 | scaffold | ncbi | 1121422 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 6247 | 48 | high performance liquid chromatography (HPLC) |
| 32137 | 48 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 82.1 | no |
| flagellated | no | 93.091 | no |
| gram-positive | no | 90.699 | no |
| anaerobic | yes | 99.631 | yes |
| aerobic | no | 97.587 | no |
| halophile | no | 96.107 | yes |
| spore-forming | yes | 95.117 | no |
| thermophile | yes | 95.41 | yes |
| glucose-util | yes | 74.812 | no |
| glucose-ferment | no | 80.645 | no |
External links
@ref: 6247
culture collection no.: DSM 16058, JCM 14019
straininfo link
- @ref: 81050
- straininfo: 343793
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6247 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16058) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16058 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 32137 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28381 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 81050 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID343793.1 | StrainInfo: A central database for resolving microbial strain identifiers |