Strain identifier
BacDive ID: 11753
Type strain: 
Species: Aggregatibacter actinomycetemcomitans
Strain history: Y. Kosako <-- R. Sakazaki <-- ATCC 29524 <-- CDC B5386 <-- Washington State Dept. Health, USA <-- Children's Orthopedic Hosp., Seattle, USA.
NCBI tax ID(s): 714 (species)
version 8.1 (current version)
General
@ref: 4214
BacDive-ID: 11753
DSM-Number: 11122
keywords: 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen
description: Aggregatibacter actinomycetemcomitans DSM 11122 is a microaerophile, mesophilic human pathogen that was isolated from chest aspirate.
NCBI tax id
- NCBI tax id: 714
- Matching level: species
strain history
| @ref | history |
|---|---|
| 4214 | <- ATCC <- CDC, B5386 <- Washington State Dept. Health <- Children's Orthopedic Hospital, Seattle |
| 67770 | Y. Kosako <-- R. Sakazaki <-- ATCC 29524 <-- CDC B5386 <-- Washington State Dept. Health, USA <-- Children's Orthopedic Hosp., Seattle, USA. |
doi: 10.13145/bacdive11753.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pasteurellales
- family: Pasteurellaceae
- genus: Aggregatibacter
- species: Aggregatibacter actinomycetemcomitans
- full scientific name: Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006
synonyms
@ref synonym 20215 Bacterium actinomycetemcomitans 20215 Haemophilus actinomycetemcomitans 20215 Actinobacillus actinomycetemcomitans
@ref: 4214
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pasteurellales
family: Pasteurellaceae
genus: Aggregatibacter
species: Aggregatibacter actinomycetemcomitans
full scientific name: Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006
type strain: no
Morphology
colony morphology
- @ref: 4214
- type of hemolysis: gamma
- incubation period: 3-7 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 4214 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 4214 | BHI MEDIUM (DSMZ Medium 215) | yes | https://mediadive.dsmz.de/medium/215 | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 4214 | positive | growth | 37 | mesophilic |
| 67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 4214
- oxygen tolerance: microaerophile
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68377 | 17634 | D-glucose | + | builds acid from |
| 68377 | 17992 | sucrose | - | builds acid from |
| 68377 | 18257 | ornithine | - | degradation |
| 68377 | 16199 | urea | - | hydrolysis |
| 68377 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 4214 | catalase | + | 1.11.1.6 |
| 4214 | cytochrome-c oxidase | - | 1.9.3.1 |
| 68377 | tryptophan deaminase | - | 4.1.99.1 |
| 68377 | beta-galactosidase | - | 3.2.1.23 |
| 68377 | urease | - | 3.5.1.5 |
| 68377 | ornithine decarboxylase | - | 4.1.1.17 |
API NH
| @ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 4214 | + | + | +/- | - | - | - | - | + | - | - | + | - | - |
| 4214 | - | + | + | +/- | - | - | - | - | + | - | - | + | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 4214 | chest aspirate | Seattle | USA | USA | North America |
| 67770 | Chest aspirate |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body-Site | #Other | #Thoracic segment |
| #Host Body Product | #Fluids | #Aspirate |
taxonmaps
- @ref: 69479
- File name: preview.99_570.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_321;97_406;98_465;99_570&stattab=map
- Last taxonomy: Aggregatibacter actinomycetemcomitans subclade
- 16S sequence: LC383929
- Sequence Identity:
- Total samples: 8336
- soil counts: 46
- aquatic counts: 279
- animal counts: 7969
- plant counts: 42
Safety information
risk assessment
- @ref: 4214
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Aggregatibacter actinomycetemcomitans strain ATCC 29524 16S ribosomal RNA gene, partial sequence | M75037 | 1485 | ena | 714 |
| 67770 | Aggregatibacter actinomycetemcomitans JCM 2435 gene for 16S ribosomal RNA, partial sequence | LC383929 | 1462 | ena | 714 |
External links
@ref: 4214
culture collection no.: DSM 11122, ATCC 29524, NCTC 10982, CDC B5386, JCM 2435
straininfo link
- @ref: 80971
- straininfo: 44836
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 1548238 | Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. | Dewhirst FE, Paster BJ, Olsen I, Fraser GJ | J Bacteriol | 10.1128/jb.174.6.2002-2013.1992 | 1992 | Base Sequence, DNA, Ribosomal/genetics, Molecular Sequence Data, Oligodeoxyribonucleotides/chemistry, Pasteurellaceae/*genetics, Phylogeny, RNA, Ribosomal, 16S/*genetics, Sequence Alignment | Genetics |
| Enzymology | 3655733 | Chemical composition and immunobiological activities of sodium dodecyl sulphate extracts from the cell envelopes of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Fusobacterium nucleatum. | Kato K, Kokeguchi S, Ishihara H, Murayama Y, Tsujimoto M, Takada H, Ogawa T, Kotani S | J Gen Microbiol | 10.1099/00221287-133-4-1033 | 1987 | Actinobacillus/*analysis, Adjuvants, Immunologic/analysis, Animals, Bacteroides/*analysis, Cell Membrane/analysis, Cell Movement, DNA/biosynthesis, Electrophoresis, Polyacrylamide Gel, Fatty Acids/analysis, Fibroblasts/metabolism, Fusobacterium/*analysis, Guinea Pigs, Humans, Mice, Sodium Dodecyl Sulfate | Pathogenicity |
| Metabolism | 6407997 | Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease. | Zambon JJ, Slots J, Genco RJ | Infect Immun | 10.1128/iai.41.1.19-27.1983 | 1983 | Actinobacillus/*classification/immunology/metabolism, Actinobacillus Infections/*microbiology, Antigens, Bacterial/immunology, Fluorescent Antibody Technique, Humans, Immunodiffusion, Periodontitis/*microbiology, Serotyping, Xylose/metabolism | Pathogenicity |
| Metabolism | 7054125 | Characterization of an inducible bacteriophage from a leukotoxic strain of Actinobacillus actinomycetemcomitans. | Stevens RH, Hammond BF, Lai CH | Infect Immun | 10.1128/iai.35.1.343-349.1982 | 1982 | *Actinobacillus/metabolism, Bacteriophages/growth & development/*isolation & purification/ultrastructure, Centrifugation, Density Gradient, DNA, Viral/analysis, Exotoxins/*biosynthesis, Virus Activation | Enzymology |
| Phylogeny | 8233565 | Multivariate chemosystematics demonstrate two groups of Actinobacillus actinomycetemcomitans strains. | Brondz I, Olsen I | Oral Microbiol Immunol | 10.1111/j.1399-302x.1993.tb00654.x | 1993 | Aggregatibacter actinomycetemcomitans/*chemistry/*classification, Bacterial Typing Techniques, Chromatography, Gas, Haemophilus/chemistry/classification, Multivariate Analysis, Pasteurella/chemistry/classification | |
| Pathogenicity | 10803129 | Absence of an especially toxic clone among isolates of Actinobacillus actinomycetemcomitans recovered from army recruits. | Macheleidt A, Muller HP, Eger T, Putzker M, Fuhrmann A, Zoller L | Clin Oral Investig | 10.1007/s007840050096 | 1999 | Adolescent, Adult, Aggregatibacter actinomycetemcomitans/*genetics/*pathogenicity, Aggressive Periodontitis/ethnology/microbiology, Bacterial Toxins/*genetics, Blacks/*genetics, Clone Cells, DNA, Bacterial/analysis, Exotoxins/*genetics, Genes, Bacterial/genetics, Humans, Military Personnel, Periodontitis/ethnology/*microbiology, Polymerase Chain Reaction, Promoter Regions, Genetic/genetics, Sequence Deletion, United States/epidemiology, Virulence, Whites/genetics | Genetics |
| Enzymology | 11981879 | Molecular genotyping of isolates of Actinobacillus actinomycetemcomitans by pulsed-field gel electrophoresis after separate digestion with Sse8387I and XhoI. | Matsuda M, Takayama K, Haga S, Kagawa S, Ohta H, Fukui K, Moore JE | J Basic Microbiol | 10.1002/1521-4028(200205)42:2<137::AID-JOBM137>3.0.CO;2-8 | 2002 | Aggregatibacter actinomycetemcomitans/classification/*genetics/*isolation & purification, Bacterial Typing Techniques/*methods, Deoxyribonucleases, Type II Site-Specific/*metabolism, *Electrophoresis, Gel, Pulsed-Field/methods, Genotype, Species Specificity | Phylogeny |
| Metabolism | 18954350 | Differential effects of five Aggregatibacter actinomycetemcomitans strains on gingival epithelial cells. | Shimada T, Sugano N, Nishihara R, Suzuki K, Tanaka H, Ito K | Oral Microbiol Immunol | 10.1111/j.1399-302X.2008.00449.x | 2008 | Actinobacillus Infections/*microbiology, Aggregatibacter actinomycetemcomitans/*pathogenicity, Cell Proliferation, Cells, Cultured, Epithelial Cells/metabolism/*microbiology, Gingiva/metabolism/*microbiology, Humans, Intercellular Adhesion Molecule-1/metabolism, Interleukin-8/metabolism, RNA, Messenger/metabolism, Species Specificity | Pathogenicity |
| Pathogenicity | 19505801 | Effect of eel galectin AJL-1 on periodontopathic bacterial biofilm formation and their lipopolysaccharide-mediated inflammatory cytokine induction. | Takayama S, Saitoh E, Kimizuka R, Yamada S, Kato T | Int J Antimicrob Agents | 10.1016/j.ijantimicag.2009.04.003 | 2009 | Aggregatibacter actinomycetemcomitans/*drug effects/growth & development, Animals, Biofilms/*drug effects, Cells, Cultured, Cytokines/drug effects/*immunology, Eels, Endothelial Cells/immunology, Galectin 1/*pharmacology, Humans, Inflammation/immunology, Lipopolysaccharides/immunology/*pharmacology, Microbial Sensitivity Tests/methods, Periodontal Diseases/*microbiology, Porphyromonas gingivalis/drug effects, Prevotella intermedia/drug effects |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 4214 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11122) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11122 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68377 | Automatically annotated from API NH | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 80971 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44836.1 | StrainInfo: A central database for resolving microbial strain identifiers |