Strain identifier

BacDive ID: 11742

Type strain: Yes

Species: Pasteurella bettyae

Strain Designation: 50, A99 and A271, A99

Strain history: CIP <- 1986, NCTC <- ATCC, Pasteurella bettii <- D.G. Hollis, CDC: strain 41-5568, Pasteurella sp. <- Florida State Hlth. Dept., USA: strain A99

NCBI tax ID(s): 1095749 (strain), 752 (species)

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General

@ref: 16645

BacDive-ID: 11742

DSM-Number: 23000

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Pasteurella bettyae 50 is a mesophilic, Gram-negative bacterium that was isolated from human Bartholin cyst.

NCBI tax id

NCBI tax idMatching level
752species
1095749strain

strain history

@refhistory
16645<- CCUG <- NCTC <- D. G. Hollis, CDC <- Florida State Dept. Health, USA
116798CIP <- 1986, NCTC <- ATCC, Pasteurella bettii <- D.G. Hollis, CDC: strain 41-5568, Pasteurella sp. <- Florida State Hlth. Dept., USA: strain A99

doi: 10.13145/bacdive11742.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Pasteurella
  • species: Pasteurella bettyae
  • full scientific name: Pasteurella bettyae corrig. Sneath and Stevens 1990

  • synonyms

    • @ref: 20215
    • synonym: Pasteurella bettii

@ref: 16645

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Pasteurella

species: Pasteurella bettyae

full scientific name: Pasteurella bettyae Sneath and Stevens 1990

strain designation: 50, A99 and A271, A99

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.923

colony morphology

  • @ref: 16645
  • type of hemolysis: gamma

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16645COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16645BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
40441MEDIUM 118 - for Pasteurella, Actinobacillus and Psychrobacter frigidicolayesDistilled water make up to (1000.000 ml);Yeast extract (1.000 g);Tryptocasein soy agar (40.000 g)
116798CIP Medium 118-byeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=118-b

culture temp

@refgrowthtypetemperaturerange
16645positivegrowth37mesophilic
40441positivegrowth37mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.987

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837717634D-glucose+builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6838129016arginine-hydrolysis
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
68377beta-galactosidase-3.2.1.23
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
44469-+---+----++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
44469-++-----+---+
16645+++/-+/----+--++-
16645-+++/-+/----+--++

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
16645-----++--------------+----------
44469-----+--------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16645human Bartholin cystFloridaUSAUSANorth America
44469Human bartholin cystFloridaUSAUSANorth America
116798Human, Bartholin cyst

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Urogenital tract#Vagina

taxonmaps

  • @ref: 69479
  • File name: preview.99_7260.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_3369;97_4137;98_5293;99_7260&stattab=map
  • Last taxonomy: Pasteurella bettyae
  • 16S sequence: AY362917
  • Sequence Identity:
  • Total samples: 194
  • soil counts: 1
  • aquatic counts: 4
  • animal counts: 189

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
166452Risk group (German classification)
1167982Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pasteurella bettyae strain CCUG 2042 T 16S ribosomal RNA gene, partial sequenceL060881476ena752
16645Pasteurella bettyae strain CCUG 2042 16S ribosomal RNA gene, partial sequenceAY3629171362ena752

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pasteurella bettyae CCUG 2042GCA_000262245contigncbi1095749
66792Pasteurella bettyae NCTC10535GCA_900454515contigncbi752
66792Pasteurella bettyae CCUG 20421095749.3wgspatric1095749
66792Pasteurella bettyae strain NCTC10535752.3wgspatric752
66792Pasteurella bettyae CCUG 20422537561960draftimg1095749

GC content

  • @ref: 16645
  • GC-content: 38.6
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno89.412no
flagellatedno96.374no
gram-positiveno98.182no
anaerobicno89.364no
aerobicno95.711no
halophileyes54.475no
spore-formingno98.705no
glucose-utilno58.898no
glucose-fermentyes61.497no
thermophileno95.994yes

External links

@ref: 16645

culture collection no.: DSM 23000, ATCC 23273, CCUG 2042, CDC 41-5568, CIP 102636, NCTC 10535

straininfo link

  • @ref: 80960
  • straininfo: 37981

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16645Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23000)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23000
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40441Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14344
44469Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2042)https://www.ccug.se/strain?id=2042
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68377Automatically annotated from API NH
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80960Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37981.1StrainInfo: A central database for resolving microbial strain identifiers
116798Curators of the CIPCollection of Institut Pasteur (CIP 102636)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102636