Strain identifier

BacDive ID: 11687

Type strain: Yes

Species: Actinobacillus succinogenes

Strain Designation: 130Z

Strain history: CIP <- 2000, ATCC <- Michigan Biotechnol. Inst., USA: Strain 130Z

NCBI tax ID(s): 67854 (species)

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General

@ref: 16239

BacDive-ID: 11687

DSM-Number: 22257

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative

description: Actinobacillus succinogenes 130Z is an aerobe, mesophilic, Gram-negative bacterium that was isolated from cattle rumen.

NCBI tax id

  • NCBI tax id: 67854
  • Matching level: species

strain history

@refhistory
16239<- CCUG <- C. Bizet, CIP <- ATCC <- ?
123367CIP <- 2000, ATCC <- Michigan Biotechnol. Inst., USA: Strain 130Z

doi: 10.13145/bacdive11687.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus succinogenes
  • full scientific name: Actinobacillus succinogenes Guettler et al. 1999

@ref: 16239

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus succinogenes

full scientific name: Actinobacillus succinogenes Guettler et al. 1999

strain designation: 130Z

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.958
123367negativerod-shapedno

colony morphology

@refincubation period
162391-2 days
123367

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16239COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16239BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
41680MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123367CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
123367CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
16239positivegrowth37mesophilic
41680positivegrowth30mesophilic
56056positivegrowth30-37mesophilic
123367positivegrowth25-37mesophilic
123367nogrowth15psychrophilic
123367nogrowth41thermophilic
123367nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56056aerobe
123367obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.996

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1233674853esculin-hydrolysis
123367606565hippurate+hydrolysis
12336717632nitrate+reduction
12336716301nitrite+reduction
6837717634D-glucose+builds acid from
6837718257ornithine-degradation

metabolite production

  • @ref: 123367
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 123367
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
16239catalase+1.11.1.6
16239cytochrome-c oxidase+1.9.3.1
68377ornithine decarboxylase-4.1.1.17
123367oxidase-
123367beta-galactosidase+3.2.1.23
123367alcohol dehydrogenase-1.1.1.1
123367catalase+1.11.1.6
123367gamma-glutamyltransferase+2.3.2.2
123367lysine decarboxylase-4.1.1.18
123367ornithine decarboxylase-4.1.1.17
123367urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123367-+++-+----++-+--+---

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16239++++--+-+-+--
16239++-+--++/-+-+--
16239++++--+++-+-+
16239++++--+++-+--
16239-++++--+-+-+-
16239-++++--+-+-+-
16239-++-+--++/-+-+-
16239-++++--+++-+-
16239+++++--+++-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123367------------------+--------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16239cattle rumen
56056Cattle rumenMichiganUSAUSANorth America
123367Animal, Cattle, rumen contentsMichiganUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Organ#Rumen

taxonmaps

  • @ref: 69479
  • File name: preview.99_5156.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_2508;97_3062;98_3843;99_5156&stattab=map
  • Last taxonomy: Actinobacillus succinogenes subclade
  • 16S sequence: AF024525
  • Sequence Identity:
  • Total samples: 1839
  • soil counts: 52
  • aquatic counts: 160
  • animal counts: 1616
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
162391Risk group (German classification)
1233671Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus succinogenes strain CCUG 43843 16S ribosomal RNA gene, partial sequenceAY3628981365ena67854
16239Actinobacillus succinogenes 16S ribosomal RNA gene, complete sequenceAF0245251488ena67854

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus succinogenes 130Z339671.7completepatric339671
66792Actinobacillus succinogenes 130Z640753001completeimg339671

GC content

  • @ref: 16239
  • GC-content: 45.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno97.955no
anaerobicno91.543yes
halophileno53.29no
spore-formingno98.843no
glucose-utilyes51.581no
motileno89.409no
flagellatedno95.639no
aerobicno96.248no
thermophileno99.331yes
glucose-fermentyes63.035no

External links

@ref: 16239

culture collection no.: DSM 22257, ATCC 55618, CCUG 43843, CIP 106512

straininfo link

  • @ref: 80907
  • straininfo: 43634

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10028265Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen.Guettler MV, Rumler D, Jain MKInt J Syst Bacteriol10.1099/00207713-49-1-2071999Actinobacillus/classification/*isolation & purification/physiology, Animals, Base Composition, Base Sequence, Cattle/*microbiology, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Rumen/*microbiology, Succinic Acid/*metabolismGenetics
Metabolism22002101Performance analyses of a neutralizing agent combination strategy for the production of succinic acid by Actinobacillus succinogenes ATCC 55618.Wang CC, Zhu LW, Li HM, Tang YJBioprocess Biosyst Eng10.1007/s00449-011-0644-62011Actinobacillus/*chemistry/*metabolism, Bioreactors/*microbiology, Carbonates/*pharmacology, Hydrogen-Ion Concentration, Succinic Acid/*metabolismBiotechnology
Metabolism22040346Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618.Zou W, Zhu LW, Li HM, Tang YJMicrob Cell Fact10.1186/1475-2859-10-872011Actinobacillus/*metabolism, Carbon Dioxide/*metabolism, Culture Media/metabolism, Fermentation, Industrial Microbiology/*methods, Magnesium/metabolism, Succinic Acid/*metabolismBiotechnology
Metabolism22112947Simultaneous saccharification and fermentation of acid-pretreated rapeseed meal for succinic acid production using Actinobacillus succinogenes.Chen K, Zhang H, Miao Y, Wei P, Chen JEnzyme Microb Technol10.1016/j.enzmictec.2010.12.0092010Actinobacillus/classification/growth & development/*metabolism, Biotechnology/*methods, Brassica rapa/*metabolism, *Carbohydrate Metabolism, Culture Media, Fermentation, Hydrogen-Ion Concentration, Polygalacturonase/metabolism, Succinic Acid/*metabolism, Sulfuric Acids/pharmacologyBiotechnology
Phylogeny23093852Actinobacillus succinogenes ATCC 55618 fermentation medium optimization for the production of succinic acid by response surface methodology.Zhu LW, Wang CC, Liu RS, Li HM, Wan DJ, Tang YJJ Biomed Biotechnol10.1155/2012/6261372012Actinobacillus/classification/*metabolism, Bioreactors/*microbiology, Computer Simulation, Culture Media/*chemistry/*metabolism, Data Interpretation, Statistical, *Models, Biological, *Models, Statistical, Species Specificity, Succinic Acid/isolation & purification/*metabolismMetabolism
28285227Fermentation and crystallization of succinic acid from Actinobacillus succinogenes ATCC55618 using fresh cassava root as the main substrate.Thuy NTH, Kongkaew A, Flood A, Boontawan ABioresour Technol10.1016/j.biortech.2017.02.1142017*Actinobacillus, Crystallization, Fermentation, Manihot, Succinic Acid
Metabolism28540516Biosuccinic Acid from Lignocellulosic-Based Hexoses and Pentoses by Actinobacillus succinogenes: Characterization of the Conversion Process.Ferone M, Raganati F, Olivieri G, Salatino P, Marzocchella AAppl Biochem Biotechnol10.1007/s12010-017-2514-42017Actinobacillus/*growth & development, Hexoses/*metabolism, Hydrolysis, Lignin/*metabolism, Pentoses/*metabolism, Succinic Acid/*metabolism
Enzymology30084002Continuous Succinic Acid Fermentation by Actinobacillus Succinogenes: Assessment of Growth and Succinic Acid Production Kinetics.Ferone M, Raganati F, Olivieri G, Salatino P, Marzocchella AAppl Biochem Biotechnol10.1007/s12010-018-2846-82018Actinobacillus/drug effects/*growth & development/*metabolism, Bioreactors, Fermentation/drug effects, Glucose/pharmacology, Kinetics, Succinic Acid/*metabolismPathogenicity
34852448Succinic acid fermentation with immobilized Actinobacillus succinogenes using hydrolysate of carbohydrate-rich microalgal biomass.Chiang YY, Nagarajan D, Lo YC, Chen CY, Ng IS, Chang CH, Lee DJ, Chang JSBioresour Technol10.1016/j.biortech.2021.1260142021*Actinobacillus, Biomass, Carbohydrates, *Chlorella vulgaris, Fermentation, *Microalgae, Succinic Acid

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16239Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22257)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22257
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41680Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18651
56056Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43843)https://www.ccug.se/strain?id=43843
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80907Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43634.1StrainInfo: A central database for resolving microbial strain identifiers
123367Curators of the CIPCollection of Institut Pasteur (CIP 106512)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106512