Strain identifier

BacDive ID: 11683

Type strain: Yes

Species: Actinobacillus seminis

Strain Designation: 5, A51 and A65, K3844/C

Strain history: CIP <- 1986, NCTC <- 1972, ATCC <- G.C. Simmons, Anim. Res. Inst., Yeerongpilly, Queensland, Australia: strain K3844/C

NCBI tax ID(s): 722 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 16243

BacDive-ID: 11683

DSM-Number: 22263

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, animal pathogen

description: Actinobacillus seminis 5 is an obligate aerobe, mesophilic, Gram-negative animal pathogen that was isolated from sheep semen.

NCBI tax id

  • NCBI tax id: 722
  • Matching level: species

strain history

@refhistory
16243<- CCUG <- NCTC <- ATCC <- G.C. Simmons
122554CIP <- 1986, NCTC <- 1972, ATCC <- G.C. Simmons, Anim. Res. Inst., Yeerongpilly, Queensland, Australia: strain K3844/C

doi: 10.13145/bacdive11683.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus seminis
  • full scientific name: Actinobacillus seminis (ex Baynes and Simmons 1960) Sneath and Stevens 1990

@ref: 16243

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus seminis

full scientific name: Actinobacillus seminis (ex Baynes and Simmons 1960) Sneath and Stevens 1990

strain designation: 5, A51 and A65, K3844/C

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.976
122554negativerod-shapedno

colony morphology

  • @ref: 16243
  • type of hemolysis: gamma

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16243BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
40386MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122554CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
16243positivegrowth37mesophilic
40386positivegrowth37mesophilic
122554positivegrowth25-41
122554nogrowth15psychrophilic
122554nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122554
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.994

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
122554esculin-hydrolysis4853
122554hippurate+hydrolysis606565
122554nitrate+reduction17632
122554nitrite-reduction16301
68377D-glucose+builds acid from17634
68377maltose-builds acid from17306
68377urea-hydrolysis16199
68377tryptophan-energy source27897

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12255435581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837735581indole-
12255415688acetoin-

enzymes

@refvalueactivityec
16243catalase-1.11.1.6
16243cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
122554oxidase-
122554beta-galactosidase+3.2.1.23
122554alcohol dehydrogenase-1.1.1.1
122554catalase+1.11.1.6
122554gamma-glutamyltransferase+2.3.2.2
122554lysine decarboxylase-4.1.1.18
122554ornithine decarboxylase+4.1.1.17
122554tryptophan deaminase-
122554urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122554-+++-+----++--++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122554+/---+/-+/-+/------+----+/-+/----------+/-------------------+/--+/-

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16243++--+-----+--
16243-++--+-----+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122554--------------------------------------+------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
16243sheep semen
48675Sheep semen
122554Animal, Sheep, semenNew South WalesAustraliaAUSAustralia and Oceania1959

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body Product#Urogenital tract#Semen

taxonmaps

  • @ref: 69479
  • File name: preview.99_158892.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_23416;97_29287;98_104605;99_158892&stattab=map
  • Last taxonomy: Actinobacillus seminis subclade
  • 16S sequence: AY362897
  • Sequence Identity:
  • Total samples: 321
  • soil counts: 5
  • aquatic counts: 11
  • animal counts: 305

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
16243yes2Risk group (German classification)
1225541Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus seminis 16S ribosomal RNA gene, partial sequence, tRNA-Glu gene, complete sequence, and 23S ribosomal RNA gene, partial sequenceAF013275562ena722
20218Actinobacillus seminis 16S ribosomal RNA gene, partial sequence, tRNA-Ile and tRNA-Ala genes, complete sequence, and 23S ribosomal RNA gene, partial sequenceAF013276691ena722
20218Actinobacillus seminis strain ATCC 15768 16S ribosomal RNA gene, partial sequenceM750471479ena722
16243Actinobacillus seminis strain CCUG 27187 16S ribosomal RNA gene, partial sequenceAY3628971364ena722

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus seminis NCTC10851GCA_900460625contigncbi722
66792Actinobacillus seminis ATCC 15768GCA_002263215contigncbi722
66792Actinobacillus seminis strain ATCC 15768722.6wgspatric722
66792Actinobacillus seminis strain NCTC10851722.7wgspatric722
66792Actinobacillus seminis NCTC 108512806310645draftimg722
66792Actinobacillus seminis ATCC 157682834290966draftimg722

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno90.614no
flagellatedno96.215no
gram-positiveno98.208no
anaerobicno97.509no
aerobicno89.281no
halophileyes60.717no
spore-formingno98.473no
glucose-utilno50.912no
thermophileno96.086yes
glucose-fermentyes58.494no

External links

@ref: 16243

culture collection no.: DSM 22263, ATCC 15768, CCUG 27187, CIP 102633, NCTC 10851, Simmons K3844-C

straininfo link

  • @ref: 80903
  • straininfo: 36478

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology7287893Cellular fatty acid and soluble protein composition of Actinobacillus actinomycetemcomitans and related organisms.Calhoon DA, Mayberry WR, Slots JJ Clin Microbiol10.1128/jcm.14.4.376-382.19811981Actinobacillus/*analysis/classification, Bacterial Proteins/*analysis, Electrophoresis, Polyacrylamide Gel, Fatty Acids/*analysis, Haemophilus/analysisPhylogeny
Phylogeny10684692Isolation of Actinobacillus seminis from the genital tract of rams in spain.de la Puente-Redondo VA, Garcia del Blanco N, Perez-Martinez C, Gonzalez-Rodriguez MC, Rodriguez-Ferri EF, Gutierrez-Martin CBJ Comp Pathol10.1053/jcpa.1999.03592000Actinobacillosis/*microbiology/pathology, Actinobacillus/drug effects/genetics/*isolation & purification, Animals, Anti-Bacterial Agents/pharmacology, DNA Fingerprinting, DNA, Bacterial/genetics, Genitalia, Male/*microbiology/pathology, Male, Microbial Sensitivity Tests, Semen/microbiology, Sheep, Spain, Testis/microbiologyEnzymology
Genetics29326222Genome Sequence of Actinobacillus seminis Strain ATCC 15768, a Reference Strain of Ovine Pathogens That Causes Infections in Reproductive Organs.Negrete-Abascal E, Montes-Garcia F, Vaca-Pacheco S, Leyto-Gil AM, Fragoso-Garcia E, Carvente-Garcia R, Perez-Agueros S, Castelan-Sanchez HG, Garcia-Molina A, Villamar TE, Sanchez-Alonso P, Vazquez-Cruz CGenome Announc10.1128/genomeA.01453-172018

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16243Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22263)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22263
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40386Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14341
48675Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 27187)https://www.ccug.se/strain?id=27187
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80903Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36478.1StrainInfo: A central database for resolving microbial strain identifiers
122554Curators of the CIPCollection of Institut Pasteur (CIP 102633)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102633