Strain identifier

BacDive ID: 11469

Type strain: Yes

Species: Paenibacillus lautus

Strain Designation: 160

Strain history: CIP <- 1988, L.K. Nakamura, NRRL <- N.R. Smith: strain NRS-666, Bacillus circulans

NCBI tax ID(s): 1349780 (strain), 1401 (species)

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General

@ref: 1277

BacDive-ID: 11469

DSM-Number: 3035

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Paenibacillus lautus 160 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from intestinal tract of child.

NCBI tax id

NCBI tax idMatching level
1349780strain
1401species

strain history

@refhistory
1277<- NRRL <- N.R. Smith, 666 (Bacillus circulans) <- B.S. Henry, 160
67770NRRL NRS-666 <-- N. R. Smith NRS-666 <-- B. S. Henry.
120573CIP <- 1988, L.K. Nakamura, NRRL <- N.R. Smith: strain NRS-666, Bacillus circulans

doi: 10.13145/bacdive11469.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus lautus
  • full scientific name: Paenibacillus lautus (Nakamura 1984 ex Batchelor 1919) Heyndrickx et al. 1996

  • synonyms

    • @ref: 20215
    • synonym: Bacillus lautus

@ref: 1277

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus lautus

full scientific name: Paenibacillus lautus (Nakamura 1984) Heyndrickx et al. 1996

strain designation: 160

type strain: yes

Morphology

cell morphology

  • @ref: 120573
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40530MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
1277NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
120573CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
120573CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
1277positivegrowth30mesophilic
40530positivegrowth30mesophilic
48770positivegrowth37mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
48770aerobe
120573facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose+builds acid from6731
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside+builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol-fermentation16899
68368D-glucose-fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
120573nitrate+reduction17632
120573nitrite-reduction16301

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
12057335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
120573oxidase-
120573catalase+1.11.1.6
120573urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120573--++-+---+-+++------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
48770---------+-----------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
48770-+-++++--+++++----+-++++++++++++++++++-++---+-----
120573+-+/-+++--+++++-+/---+-+/-++++++++++++-++++-++---+/---+/---

Isolation, sampling and environmental information

isolation

@refsample type
1277intestinal tract of child
67770Human intestinaltract
120573Human, Child, intestinal tract

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body-Site#Gastrointestinal tract
#Host#Human#Child

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
12771Risk group (German classification)
1205731Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Paenibacillus lautus clone Blautus-1R 16S ribosomal RNA gene, and 16S-23S internal transcribed spacer, partial sequenceAF478100663ena1401
20218Paenibacillus lautus DNA for 16S ribosomal RNAD784721436ena1401
20218Paenibacillus lautus gene for 16S rRNA, partial sequenceAB0731881505ena1401
20218Paenibacillus lautus partial 16S rRNA gene, strain JCM 9073, isolate AB236dFR7754381437ena1401
20218Paenibacillus lautus gene for 16S rRNA, partial sequence, strain: NBRC 15380AB3637331480ena1401
20218B.lautus 16S ribosomal RNAX606211430ena85381
20218Paenibacillus lautus DNA for 16S rRNAD784731436ena1401

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paenibacillus lautus NBRC 153801349780.3wgspatric1349780
67770Paenibacillus lautus NBRC 15380GCA_004000945contigncbi1349780

GC content

@refGC-contentmethod
127751.5
6777051Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes91.676no
gram-positiveyes83.588no
anaerobicno97.713yes
halophileno81.448no
spore-formingyes95.538no
glucose-utilyes87.921no
thermophileno99.43no
flagellatedyes77.774no
aerobicyes88.219yes
glucose-fermentno88.14no

External links

@ref: 1277

culture collection no.: DSM 3035, ATCC 43898, LMG 11157, NCIMB 12780, CCUG 27414, NRRL NRS-666, CCM 3892, CIP 103118, JCM 9073, NRS 666, IFO 15380, KCTC 3456, NBRC 15380

straininfo link

  • @ref: 80703
  • straininfo: 125606

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24611959Isolation and characterization of chitinase-producing Bacillus and Paenibacillus strains from salted and fermented shrimp, Acetes japonicus.Han KI, Patnaik BB, Kim YH, Kwon HJ, Han YS, Han MDJ Food Sci10.1111/1750-3841.123872014Animals, Bacillus/*enzymology/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Bioreactors, Chitin/*metabolism, Chitinases/genetics/*metabolism, DNA, Bacterial/genetics, Decapoda/*microbiology, Fatty Acids/analysis, Fermentation, *Food Microbiology, Genes, rRNA, Humans, Nucleic Acid Hybridization, Paenibacillus/*enzymology/genetics/isolation & purification, Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood/*microbiology, Sequence Analysis, DNA, Sodium Chloride, Spores, Bacterial/geneticsEnzymology
Phylogeny25288280Paenibacillus lemnae sp. nov., an endophytic bacterium of duckweed (Lemna aequinoctialis).Kittiwongwattana C, Thawai CInt J Syst Evol Microbiol10.1099/ijs.0.067876-02014Araceae/*microbiology, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, ThailandGenetics
Phylogeny25858246Paenibacillus qingshengii sp. nov., isolated from a lead-zinc tailing.Chen L, Wang L, Sheng XFInt J Syst Evol Microbiol10.1099/ijs.0.0002322015Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Mining, Molecular Sequence Data, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry, ZincGenetics
Phylogeny26373452Paenibacillus zeae sp. nov., isolated from maize (Zea mays L.) seeds.Liu Y, Zhai L, Wang R, Zhao R, Zhang X, Chen C, Cao Y, Cao Y, Xu T, Ge Y, Zhao J, Cheng CInt J Syst Evol Microbiol10.1099/ijsem.0.0006082015Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Endophytes/classification/genetics/isolation & purification, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seeds/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, Zea mays/*microbiologyEnzymology
Phylogeny27498790Paenibacillus solani sp. nov., isolated from potato rhizosphere soil.Liu B, Liu GH, Sengonca C, Schumann P, Lan JL, Chen DJ, Cui WD, Lin NQInt J Syst Evol Microbiol10.1099/ijsem.0.0013782016Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Solanum tuberosum/*microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1277Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3035)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3035
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40530Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14881
48770Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 27414)https://www.ccug.se/strain?id=27414
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
80703Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125606.1StrainInfo: A central database for resolving microbial strain identifiers
120573Curators of the CIPCollection of Institut Pasteur (CIP 103118)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103118