Strain identifier
BacDive ID: 11149
Type strain: 
Species: Nocardioides mesophilus
Strain Designation: MSL-22
Strain history: <- CJ Kim, KRIBB
NCBI tax ID(s): 433659 (species)
version 8.1 (current version)
General
@ref: 8012
BacDive-ID: 11149
DSM-Number: 19432
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped
description: Nocardioides mesophilus MSL-22 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 433659
- Matching level: species
strain history
| @ref | history |
|---|---|
| 8012 | <- C. J. Kim, Korea Res. Inst. Biosci. & Biotechnol. (KRIBB), Taejon, South Korea <- Y. J. Ju; MSL-22 |
| 67771 | <- CJ Kim, KRIBB |
doi: 10.13145/bacdive11149.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Propionibacteriales
- family: Nocardioidaceae
- genus: Nocardioides
- species: Nocardioides mesophilus
- full scientific name: Nocardioides mesophilus Dastager et al. 2010
@ref: 8012
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Nocardioidaceae
genus: Nocardioides
species: Nocardioides mesophilus
full scientific name: Nocardioides mesophilus Dastager et al. 2010
strain designation: MSL-22
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility |
|---|---|---|---|---|---|
| 29703 | positive | 0.9-1.4 µm | 0.3-0.8 µm | rod-shaped | yes |
| 67771 | positive |
pigmentation
- @ref: 29703
- production: yes
Culture and growth conditions
culture medium
- @ref: 8012
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 8012 | positive | growth | 28 | mesophilic |
| 29703 | positive | growth | 20-37 | |
| 29703 | positive | optimum | 28 | mesophilic |
| 67771 | positive | growth | 30 | mesophilic |
culture pH
- @ref: 29703
- ability: positive
- type: optimum
- pH: 7-7.5
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 29703 | aerobe |
| 67771 | aerobe |
spore formation
- @ref: 29703
- spore formation: no
observation
- @ref: 29703
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29703 | 29864 | mannitol | + | carbon source |
| 29703 | 16634 | raffinose | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29703 | acid phosphatase | + | 3.1.3.2 |
| 29703 | alkaline phosphatase | + | 3.1.3.1 |
| 29703 | catalase | + | 1.11.1.6 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 8012 | soil | Bigeum Island, Jeollanam-do Province | Republic of Korea | KOR | Asia |
| 67771 | From farming field | Bikeum island | Republic of Korea | KOR | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 8012
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 8012
- description: Nocardioides mesophilus strain MSL 22 16S ribosomal RNA gene, partial sequence
- accession: EF466117
- length: 1403
- database: ena
- NCBI tax ID: 433659
GC content
| @ref | GC-content | method |
|---|---|---|
| 8012 | 68.7 | high performance liquid chromatography (HPLC) |
| 29703 | 68.7 |
External links
@ref: 8012
culture collection no.: DSM 19432, KCTC 19310, MSL 22, NBRC 107606
straininfo link
- @ref: 80398
- straininfo: 370984
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19915109 | Nocardioides mesophilus sp. nov., isolated from soil. | Dastager SG, Lee JC, Pandey A, Kim CJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.019059-0 | 2009 | Actinomycetales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Korea, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Temperature, Vitamin K 2/analysis | Genetics |
| Phylogeny | 20935090 | Nocardioides iriomotensis sp. nov., an actinobacterium isolated from forest soil. | Yamamura H, Ohkubo SY, Nakagawa Y, Ishida Y, Hamada M, Otoguro M, Tamura T, Hayakawa M | Int J Syst Evol Microbiol | 10.1099/ijs.0.025080-0 | 2010 | Actinomycetales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Japan, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Trees | Genetics |
| Phylogeny | 21742819 | Nocardioides daejeonensis sp. nov., a denitrifying bacterium isolated from sludge in a sewage-disposal plant. | Woo SG, Srinivasan S, Yang J, Jung YA, Kim MK, Lee M | Int J Syst Evol Microbiol | 10.1099/ijs.0.033308-0 | 2011 | Actinomycetales/*classification/genetics/*isolation & purification/physiology, Aerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Denitrification, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Vitamin K 2/analysis | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 8012 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19432) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19432 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29703 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26086 | 28776041 | |
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
| 80398 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID370984.1 | StrainInfo: A central database for resolving microbial strain identifiers |