Strain identifier

BacDive ID: 11058

Type strain: Yes

Species: Aeromicrobium alkaliterrae

Strain Designation: KSL-107

Strain history: CIP <- 2005, DSMZ <- J-H Yoon, KRIBB, Taejon, Korea: strain KSL-107

NCBI tax ID(s): 302168 (species)

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General

@ref: 6616

BacDive-ID: 11058

DSM-Number: 16824

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped

description: Aeromicrobium alkaliterrae KSL-107 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 302168
  • Matching level: species

strain history

@refhistory
6616<- Jung-Hoon Yoon, KSL-107
67770DSM 16824 <-- J.-H. Yoon KSL-107.
67771<- JH Yoon, KRIBB
116249CIP <- 2005, DSMZ <- J-H Yoon, KRIBB, Taejon, Korea: strain KSL-107

doi: 10.13145/bacdive11058.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Nocardioidaceae
  • genus: Aeromicrobium
  • species: Aeromicrobium alkaliterrae
  • full scientific name: Aeromicrobium alkaliterrae Yoon et al. 2005

@ref: 6616

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardioidaceae

genus: Aeromicrobium

species: Aeromicrobium alkaliterrae

full scientific name: Aeromicrobium alkaliterrae Yoon et al. 2005

strain designation: KSL-107

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31446positive1.25 µm1.25 µmcoccus-shapedno
67771positive
116249positiveoval-shapedno

colony morphology

@refcolony colorincubation periodmedium used
18405Beige (1001)10-14 daysISP 2
18405Beige (1001)10-14 daysISP 3
18405Beige (1001)10-14 daysISP 4
1840510-14 daysISP 5
18405Beige (1001)10-14 daysISP 6
1840510-14 daysISP 7
116249

multicellular morphology

@refforms multicellular complexmedium name
18405noISP 2
18405noISP 3
18405noISP 4
18405noISP 5
18405noISP 6
18405noISP 7

pigmentation

  • @ref: 31446
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6616TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18405ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18405ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18405ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18405ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18405ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18405ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
6616TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
116249CIP Medium 338yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=338

culture temp

@refgrowthtypetemperaturerange
6616positivegrowth28mesophilic
18405positiveoptimum28mesophilic
31446positivegrowth4.0-35.0
31446positiveoptimum19.5psychrophilic
67770positivegrowth28mesophilic
67771positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
31446positivegrowth6.0-11.0alkaliphile
31446positiveoptimum7.25

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31446aerobe
67771aerobe
116249obligate aerobe

spore formation

  • @ref: 31446
  • spore formation: no

halophily

  • @ref: 31446
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-9 %

observation

@refobservation
31446aggregates in clumps
67770quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3144629016arginine+carbon source
3144617057cellobiose+carbon source
3144628260galactose+carbon source
3144625115malate+carbon source
3144630031succinate+carbon source
3144653424tween 20+carbon source
3144653423tween 40+carbon source
3144653425tween 60+carbon source
3144653426tween 80+carbon source
11624917632nitrate-reduction
11624916301nitrite-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917992sucrose+fermentation

metabolite production

@refChebi-IDmetaboliteproduction
3144616136hydrogen sulfideyes
3144635581indoleyes
11624935581indoleno

metabolite tests

  • @ref: 31446
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
116249oxidase-
116249alcohol dehydrogenase-1.1.1.1
116249catalase+1.11.1.6
116249lysine decarboxylase-4.1.1.18
116249ornithine decarboxylase-4.1.1.17
116249urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELSAC
18405-++--+--+-+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18405+++++--+++----+----
116249++++++---++---+-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6616soilKwangchunRepublic of KoreaKORAsia
67770Alkaline soilKwangchunRepublic of KoreaKORAsia
67771From soilRepublic of KoreaKORAsia
116249Environment, Alkaline soilKwangchunRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
66161Risk group (German classification)
184051German classification
1162491Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6616
  • description: Aeromicrobium alkaliterrae 16S ribosomal RNA gene, partial sequence
  • accession: AY822044
  • length: 1473
  • database: ena
  • NCBI tax ID: 302168

GC content

@refGC-contentmethod
661671.5
6777071.5high performance liquid chromatography (HPLC)

External links

@ref: 6616

culture collection no.: DSM 16824, CIP 108938, JCM 13518, KCTC 19073, BCRC 16830

straininfo link

  • @ref: 80307
  • straininfo: 232306

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16166727Aeromicrobium alkaliterrae sp. nov., isolated from an alkaline soil, and emended description of the genus Aeromicrobium.Yoon JH, Lee CH, Oh TKInt J Syst Evol Microbiol10.1099/ijs.0.63582-02005Actinomycetales/chemistry/*classification/genetics/isolation & purification, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Hydrogen-Ion Concentration, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny26354190Aeromicrobium camelliae sp. nov., isolated from Pu'er tea.Niu L, Xiong M, Tang T, Song L, Hu X, Zhao M, Zhang KInt J Syst Evol Microbiol10.1099/ijsem.0.0005832015Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tea/*microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6616Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16824)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16824
18405Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM16824.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31446Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2774928776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
80307Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID232306.1StrainInfo: A central database for resolving microbial strain identifiers
116249Curators of the CIPCollection of Institut Pasteur (CIP 108938)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108938