Strain identifier

BacDive ID: 10906

Type strain: No

Species: Rhodococcus equi

Strain Designation: S577, 545

Strain history: CIP <- 2001, CCUG <- 1985, DSMZ <- NCIB <- ATCC, Corynebacterium sp. <- R.E. Gordon: strain 545 <- E. Azarowicz <- CBS

NCBI tax ID(s): 1831 (species)

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General

@ref: 10863

BacDive-ID: 10906

DSM-Number: 43199

keywords: 16S sequence, obligate aerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Rhodococcus equi S577 is an obligate aerobe, mesophilic, Gram-positive human pathogen.

NCBI tax id

  • NCBI tax id: 1831
  • Matching level: species

strain history

@refhistory
10863<- NCIB <- ATCC <- R.E. Gordon, 545 (Corynebacterium sp.) <- E. Azarowicz (Nocardia restricta) <- CBS
67770KCC A-0223 <-- DSM 43199 <-- NCIB 10027 <-- ATCC 14887 <-- R. E. Gordon 545.
119113CIP <- 2001, CCUG <- 1985, DSMZ <- NCIB <- ATCC, Corynebacterium sp. <- R.E. Gordon: strain 545 <- E. Azarowicz <- CBS

doi: 10.13145/bacdive10906.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • genus: Rhodococcus
  • species: Rhodococcus equi
  • full scientific name: Rhodococcus equi (Magnusson 1923) Goodfellow and Alderson 1977 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Prescottella equi
    20215Corynebacterium equi
    20215Nocardia restricta
    20215Proactinomyces restrictus
    20215Prescottia equi

@ref: 10863

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Rhodococcus

species: Rhodococcus sp.

full scientific name: Rhodococcus sp.

strain designation: S577, 545

type strain: no

Morphology

cell morphology

  • @ref: 119113
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 119113

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10863GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
36024MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119113CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
10863positivegrowth28mesophilic
36024positivegrowth30mesophilic
67770positivegrowth28mesophilic
119113positivegrowth25-37mesophilic
119113nogrowth10psychrophilic
119113nogrowth41thermophilic
119113nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119113
  • oxygen tolerance: obligate aerobe

halophily

  • @ref: 119113
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
11911316947citrate-carbon source
1191134853esculin-hydrolysis
119113606565hippurate-hydrolysis
11911317632nitrate-reduction
11911316301nitrite-reduction
11911317632nitrate-respiration
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 119113
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11911335581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11911315688acetoin-
11911317234glucose-

enzymes

@refvalueactivityec
68379catalase+1.11.1.6
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
119113oxidase-
119113beta-galactosidase-3.2.1.23
119113alcohol dehydrogenase-1.1.1.1
119113gelatinase-
119113amylase-
119113DNase-
119113caseinase-3.4.21.50
119113catalase+1.11.1.6
119113tween esterase+
119113gamma-glutamyltransferase-2.3.2.2
119113lecithinase-
119113lipase-
119113lysine decarboxylase-4.1.1.18
119113ornithine decarboxylase-4.1.1.17
119113phenylalanine ammonia-lyase-4.3.1.24
119113tryptophan deaminase-
119113urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    46683C14:04.914
    46683C15:01.515
    46683C16:034.316
    46683C18:02.218
    46683C16:0 10-methyl1.616.433
    46683C16:1 ω7c2.915.819
    46683C16:1 ω7c/C15:0 ISO 2OH10.615.85
    46683C17:1 ω8c1.516.792
    46683C18:1 ω9c15.417.769
    46683C18:2 ω6,9c/C18:0 ANTE6.417.724
    46683C20:2 ω6,9c1.619.735
    46683TBSA 10Me18:06.318.392
    46683Unidentified10.919.015
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
46683+-++--+-++----------+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
46683-+---++---++----+---
10863-+---++---++---+/-+---
119113-+-+-++---++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
10863----------------+/-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119113+-----------+------+-----------+-----------------+------------++----++--+--+---++-------+-+-----+--

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_58.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_14;97_50;98_54;99_58&stattab=map
  • Last taxonomy: Rhodococcus
  • 16S sequence: X80613
  • Sequence Identity:
  • Total samples: 31273
  • soil counts: 18366
  • aquatic counts: 4265
  • animal counts: 5588
  • plant counts: 3054

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
10863yes, in single cases1Risk group (German classification)
1191132Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Rhodococcus equi 16S rRNA gene, strain DSM43199T
  • accession: X80613
  • length: 1475
  • database: ena
  • NCBI tax ID: 43767

External links

@ref: 10863

culture collection no.: DSM 43199, ATCC 14887, ATCC 25715, CCRC 11929, CCUG 17758, KCC A-0223, NCIB 10027, VKM Ac-957, CIP 107070, JCM 3223, BCRC 11929, IFM 0220, IMET 7279, LMG 7335, MTCC 2558, NCIMB 10027

straininfo link

  • @ref: 80160
  • straininfo: 92422

literature

  • topic: Metabolism
  • Pubmed-ID: 17351967
  • title: Steroid 9alpha-hydroxylation during testosterone degradation by resting rhodococcus equi cells.
  • authors: Kim YU, Han J, Lee SS, Shimizu K, Tsutsumi Y, Kondo R
  • journal: Arch Pharm (Weinheim)
  • DOI: 10.1002/ardp.200600175
  • year: 2007
  • mesh: Androstanes/metabolism, Biotransformation, Culture Media, Hydroxylation, Kinetics, Magnetic Resonance Spectroscopy, Mass Spectrometry, Rhodococcus equi/*metabolism, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Steroid Hydroxylases/*metabolism, Testosterone/*metabolism
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10863Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43199)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43199
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36024Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19270
46683Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 17758)https://www.ccug.se/strain?id=17758
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80160Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92422.1StrainInfo: A central database for resolving microbial strain identifiers
119113Curators of the CIPCollection of Institut Pasteur (CIP 107070)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107070