Name: Rhodococcus equi S577
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 10906

Type strain:

Species: Rhodococcus equi

Strain Designation: S577, 545

Culture col. no.: DSM 43199, ATCC 14887, ATCC 25715, CCRC 11929, CCUG 17758, KCC A-0223, NCIB 10027, VKM Ac-957, CIP 107070, JCM 3223, BCRC 11929, IFM 0220, IMET 7279, LMG 7335, MTCC 2558, NCIMB 10027

Strain history: CIP <- 2001, CCUG <- 1985, DSMZ <- NCIB <- ATCC, Corynebacterium sp. <- R.E. Gordon: strain 545 <- E. Azarowicz <- CBS

NCBI tax ID(s): 1831 (species)

SI-ID 92422

LMG 7335, ATCC 14887, DSM 43199, ICMP 4147, NCIB 10027, PDDCC 4147, CCUG 17758, ATCC 25715, CCRC 11929, JCM 3223, IMET 7279, NCIMB 10027, VKM Ac-957, CIP 107070, BCRC 11929, DSM 26605

Other strains from Rhodococcus equi

R. equi 304-74, CIP 74.35
R. equi 165-87, CIP 102900
R. equi 787-80, CIP 104292
R. equi 3158, 413-86, CIP 102409
R. equi CIP 102613
R. equi 16.111, CIP 58.70
R. equi 16449, CIP 58.71
R. equi Cattin, 219-79, CIP 79.36
R. equi CIP 101849
R. equi CIP 102374
R. equi 6600, CIP 58.69
R. equi GK1, CIP 105335

Section

Rhodococcus equi S577 is an obligate aerobe, mesophilic, Gram-positive human pathogen.

Gram-positive
rod-shaped
human pathogen
mesophilic
obligate aerobe
16S sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Genus	*Rhodococcus*
	Species	**Rhodococcus equi**
	Full Scientific Name (LPSN)	Rhodococcus equi (Magnusson 1923) Goodfellow and Alderson 1977 (Approved Lists 1980)

	Synonym	"Prescottella* equi"*
	Synonym	Corynebacterium equi
	Synonym	Nocardia restricta
	Synonym	"Proactinomyces* restrictus"*
	Synonym	"Prescottia* equi"*

Information on morphological and physiological properties Morphology

[Ref.: #119113]	Gram stain	positive
[Ref.: #119113]	Cell shape	rod-shaped
[Ref.: #119113]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #10863]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #10863]

Culture medium growth

[Ref.: #10863]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10863]

Culture medium composition

expand / minimize

[Ref.: #36024]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36024]

Culture medium growth

[Ref.: #36024]

Culture medium composition

expand / minimize

[Ref.: #119113]

Culture medium

CIP Medium 72

[Ref.: #119113]

Culture medium growth

[Ref.: #119113]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #10863]		growth	28 °C
[Ref.: #36024]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #119113]		growth	25-37 °C
[Ref.: #119113]	no	growth	10 °C
[Ref.: #119113]	no	growth	41 °C
[Ref.: #119113]	no	growth	45 °C

[Ref.: #10863]	Temperature range	mesophilic
[Ref.: #36024]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #119113]	Temperature range	mesophilic
[Ref.: #119113]	Temperature range	psychrophilic
[Ref.: #119113]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119113]

Oxygen tolerance

obligate aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #119113]		NaCl		growth	0-10	%

[Ref.: #67770]

Observation

quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #119113]	citrate ChEBI	-	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE, API Coryne
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #119113]	esculin ChEBI	-	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE, API Coryne
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #119113]	hippurate ChEBI	-	hydrolysis
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #119113]	nitrate ChEBI	-	reduction
[Ref.: #119113]	nitrate ChEBI	-	respiration
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #119113]	nitrite ChEBI	-	reduction
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119113]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #119113]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #119113]	acetoin ChEBI	-
[Ref.: #119113]	glucose ChEBI		-
[Ref.: #68369]	indole ChEBI			-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119113]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119113]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #119113]	beta-galactosidase	-	3.2.1.23
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #119113]	caseinase	-	3.4.21.50
[Ref.: #119113]	catalase	+	1.11.1.6
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #119113]	DNase	-
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #119113]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #119113]	gelatinase	-
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20NE
[Ref.: #119113]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119113]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119113]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119113]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119113]	oxidase	-
[Ref.: #119113]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119113]	tryptophan deaminase	-
[Ref.: #119113]	tween esterase	+
[Ref.: #119113]	urease	+	3.5.1.5
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #46683]

Fatty acid	Percentage	ECL
C16:0	34.3	16
C18:1 ω9c	15.4	17.769
Unidentified	10.9	19.015
C16:1 ω7c/C15:0 ISO 2OH	10.6	15.85
C18:2 ω6,9c/C18:0 ANTE	6.4	17.724
TBSA 10Me18:0	6.3	18.392
C14:0	4.9	14
C16:1 ω7c	2.9	15.819
C18:0	2.2	18
C16:0 10-methyl	1.6	16.433
C20:2 ω6,9c	1.6	19.735
C17:1 ω8c	1.5	16.792
C15:0	1.5	15

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #10863]	25126	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	+	-	-	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #119113]	1984	not determinedn.d.	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	-	+	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	+	-	-

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #46683]	797	+	-	+	+	-	-	+	-	+	+	-	-	-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #46683]	1525	-	+	-	-	-	+	+	-	-	-	+	+	-	-	-	-	+	-	-	-
[Ref.: #10863]	14257	-	+	-	-	-	+	+	-	-	-	+	+	-	-	-	+/-	+	-	-	-
[Ref.: #119113]	10038	-	+	-	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence X80613 (>99% sequence identity) for Rhodococcus from Microbeatlas

Aquatic Samples	4265
Soil Samples	18366
Animal Samples	5588
Plant Samples	3054
Total Samples	31273

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #10863]	Pathogenicity (human)	yes, in single cases
[Ref.: #10863]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119113]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Rhodococcus equi 16S rRNA gene, strain DSM43199T	X80613	1475	ENA	43767 tax ID	*

Availability in culture collections External links

[Ref.: #10863]

Culture collection no.

DSM 43199, ATCC 14887, ATCC 25715, CCRC 11929, CCUG 17758, KCC A-0223, NCIB 10027, VKM Ac-957, CIP 107070, JCM 3223, BCRC 11929, IFM 0220, IMET 7279, LMG 7335, MTCC 2558, NCIMB 10027

[Ref.: #80160]

SI-ID 92422

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Steroid 9alpha-hydroxylation during testosterone degradation by resting rhodococcus equi cells.	Kim YU, Han J, Lee SS, Shimizu K, Tsutsumi Y, Kondo R	Arch Pharm (Weinheim)	10.1002/ardp.200600175	2007	*

References

#10863

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43199

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36024 ; Curators of the CIP;
#46683 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 17758
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80160

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92422.1 )

#119113 Collection of Institut Pasteur ; Curators of the CIP; CIP 107070
* These data were automatically processed and therefore are not curated

Change proposal

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