Strain identifier

BacDive ID: 10461

Type strain: Yes

Species: Kingella kingae

Strain Designation: 4177/66

Strain history: CIP <- 1980, NCTC <- 1967, K. Boevre, Oslo, Norway: strain 4177/66, Moraxella kingae

NCBI tax ID(s): 887327 (strain), 504 (species)

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General

@ref: 3208

BacDive-ID: 10461

DSM-Number: 7536

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Kingella kingae 4177/66 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human nose.

NCBI tax id

NCBI tax idMatching level
887327strain
504species

strain history

@refhistory
3208<- ATCC <- S.D. Henriksen and K. Bovre, 4177/66
120414CIP <- 1980, NCTC <- 1967, K. Boevre, Oslo, Norway: strain 4177/66, Moraxella kingae

doi: 10.13145/bacdive10461.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Kingella
  • species: Kingella kingae
  • full scientific name: Kingella kingae (Henriksen and Bøvre 1968) Henriksen and Bøvre 1976 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Moraxella kingii

@ref: 3208

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Kingella

species: Kingella kingae

full scientific name: Kingella kingae (Henriksen and Bøvre 1968) Henriksen and Bøvre 1976

strain designation: 4177/66

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.984

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3208COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
37715MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120414CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
3208positivegrowth37mesophilic
37715positivegrowth30mesophilic
44171positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 44171
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no98
69480no100

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377sucrose+builds acid from17992
68377ornithine-degradation18257
68377urea-hydrolysis16199
68377tryptophan-energy source27897

metabolite production

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase+2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase+3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120414-------------------------------------------------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3208-++++---+--+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120414---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
3208human noseOsloNorwayNOREurope
44171Human nose1966
120414Human, NoseOsloNorwayNOREurope1966

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Nose

taxonmaps

  • @ref: 69479
  • File name: preview.99_2241.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_1209;97_1433;98_1746;99_2241&stattab=map
  • Last taxonomy: Kingella kingae subclade
  • 16S sequence: M22517
  • Sequence Identity:
  • Total samples: 422
  • soil counts: 12
  • aquatic counts: 10
  • animal counts: 399
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
32082Risk group (German classification)
1204141Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Kingella kingae ATCC:23330 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceFJ4103923579ena887327
20218Kingella kingae 16S ribosomal RNA, complete sequenceM225171476ena887327
20218Kingella kingae 16S rRNA gene, partialAJ247216453ena887327
20218K.kingii gene for 16S rRNAX74902719ena887327
3208Kingella kingae strain ATCC 23330 16S ribosomal RNA gene, partial sequenceAY5519991482ena887327

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kingella kingae NCTC10529GCA_900475905completencbi504
66792Kingella kingae ATCC 23330GCA_900111845contigncbi887327
66792Kingella kingae ATCC 23330GCA_000213535scaffoldncbi887327
66792Kingella kingae ATCC 23330887327.3wgspatric887327
66792Kingella kingae ATCC 23330887327.11wgspatric887327
66792Kingella kingae strain NCTC10529504.101completepatric504
66792Kingella kingae NCTC 105292841829122completeimg504
66792Kingella kingae ATCC 23330651324044draftimg887327
66792Kingella kingae ATCC 233302850297160draftimg887327
66792Kingella kingae 4177/66GCA_022871005completencbi504

GC content

  • @ref: 3208
  • GC-content: 48.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
flagellatedno95.034no
gram-positiveno98.311no
anaerobicno97.763yes
aerobicno95.234no
halophileno75.133no
spore-formingno96.49no
thermophileno98.758yes
glucose-utilyes61.333no
motileno86.398no
glucose-fermentno88.753no

External links

@ref: 3208

culture collection no.: CCUG 352, NCTC 10529, DSM 7536, ATCC 23330, CIP 80.16, CCM 5679, JCM 1479

straininfo link

  • @ref: 79738
  • straininfo: 37994

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28699877Isolation and characterization of Kingella negevensis sp. nov., a novel Kingella species detected in a healthy paediatric population.El Houmami N, Bakour S, Bzdrenga J, Rathored J, Seligmann H, Robert C, Armstrong N, Schrenzel J, Raoult D, Yagupsky P, Fournier PEInt J Syst Evol Microbiol10.1099/ijsem.0.0019572017Bacterial Typing Techniques, Base Composition, Child, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Humans, Israel, Kingella/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Oropharynx/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwitzerlandPathogenicity
Phylogeny36309905Kingella pumchi sp. nov., an organism isolated from human vertebral puncture tissue.Xiao M, Liu R, Du J, Liu R, Zhai L, Wang H, Yao S, Xu YCAntonie Van Leeuwenhoek10.1007/s10482-022-01786-w2022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3208Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7536)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7536
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37715Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11108
44171Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 352)https://www.ccug.se/strain?id=352
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
79738Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37994.1StrainInfo: A central database for resolving microbial strain identifiers
120414Curators of the CIPCollection of Institut Pasteur (CIP 80.16)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2080.16