Strain identifier

BacDive ID: 159864

Type strain: Yes

Species: Amycolatopsis antarctica

Strain Designation: AU-G6

NCBI tax ID(s): 1854586 (species)

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General

@ref: 66596

BacDive-ID: 159864

keywords: 16S sequence, aerobe, mesophilic, gram-positive, rod-shaped

description: Amycolatopsis antarctica AU-G6 is an aerobe, mesophilic, gram-positive archaeon that builds a substrate mycelium and was isolated from surface of an antarctic brown macroalga Adenocystis utricularis.

doi: 10.13145/bacdive159864.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Amycolatopsis
  • species: Amycolatopsis antarctica
  • full scientific name: Amycolatopsis antarctica Wang et al. 2018

@ref: 66596

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Amycolatopsis

species: Amycolatopsis antarctica

strain designation: AU-G6

type strain: yes

Morphology

cell morphology

  • @ref: 66596
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

multicellular morphology

  • @ref: 66596
  • forms multicellular complex: yes
  • complex name: substrate mycelium
  • complex color: ivory to beige brown
  • medium name: different ISP agar media

Culture and growth conditions

culture medium

@refnamegrowth
66596Czapek's agaryes
66596ISP 2yes
66596ISP 3yes
66596ISP 4yes
66596ISP 5yes
66596ISP 6yes
66596ISP 7yes
66596modified Bennett's agaryes
66596potato-dextrose agaryes

culture temp

@refgrowthtypetemperaturerange
66596nogrowth37mesophilic
66596positiveoptimum15-20psychrophilic
66596positivegrowth4-28

culture pH

@refabilitytypepH
66596nogrowth5
66596nogrowth11
66596positivegrowth6-10
66596positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 66596
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
66596NaClpositivegrowth0.5-7.0 %(w/v)
66596NaClnogrowth10 %(w/v)
66596NaClpositiveoptimum3.0-5.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6659616411acetic acid-carbon source
6659630623oxalate-carbon source
66596132950tartrate-carbon source
6659615443inulin+carbon source
6659615824D-fructose+carbon source
6659616813galactitol+carbon source
6659616899D-mannitol+carbon source
6659617268myo-inositol+carbon source
6659617306maltose+carbon source
6659617634D-glucose+carbon source
6659617716lactose+carbon source
6659617754glycerol+carbon source
6659617924D-sorbitol+carbon source
6659617992sucrose+carbon source
6659627082trehalose+carbon source
6659628017starch+carbon source
6659630031succinate+carbon source
6659630849L-arabinose+carbon source
6659652071dextran+carbon source
6659653258citric acid+carbon source
6659662345L-rhamnose+carbon source
6659665327D-xylose+carbon source
665964853esculin+/-carbon source
665966731melezitose+/-carbon source
6659612936D-galactose+/-carbon source
6659616024D-mannose+/-carbon source
6659616634raffinose+/-carbon source
6659617113erythritol+/-carbon source
6659617814salicin+/-carbon source
6659623652dextrin+/-carbon source
6659628053melibiose+/-carbon source
6659635696cobalt dichloride-growth
6659649976zinc dichloride-growth
66596278547sodium azide-growth
6659663317barium chloride+growth
6659675248potassium tellurite+/-growth
6659616235guanine-hydrolysis
6659616708adenine-hydrolysis
6659617309pectin-hydrolysis
6659617368hypoxanthine-hydrolysis
6659628017starch-hydrolysis
6659637166xylan-hydrolysis
6659685146carboxymethylcellulose-hydrolysis
665964853esculin+hydrolysis
665965291gelatin+hydrolysis
6659615676allantoin+hydrolysis
6659618305arbutin+hydrolysis
6659653423tween 40+hydrolysis
6659653424tween 20+hydrolysis
6659653425tween 60+hydrolysis
6659653426tween 80+hydrolysis
66596606565hippurate+hydrolysis
66596casein+hydrolysis
6659615318xanthine+/-hydrolysis
6659616199urea+/-hydrolysis
6659617029chitin+/-hydrolysis
6659616643L-methionine-nitrogen source
6659615428glycine+nitrogen source
6659615971L-histidine+nitrogen source
6659616414L-valine+nitrogen source
6659616467L-arginine+nitrogen source
6659616977L-alanine+nitrogen source
6659617115L-serine+nitrogen source
6659617203L-proline+nitrogen source
6659617295L-phenylalanine+nitrogen source
6659617561L-cysteine+nitrogen source
6659624741hydroxyproline+nitrogen source
6659624898isoleucine+/-nitrogen source
6659617632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6659617698chloramphenicolyesyes30 µg (disc)
6659648923erythromycinyesyes15 µg (disc)
6659629687teicoplaninyesyes30 µg (disc)
6659618208penicillin gyesyes10 Unit (disc)
6659627902tetracyclineyesyes30 µg (disc)
6659628001vancomycinyesyes30 µg (disc)
6659628077rifampicinyesyes5 µg (disc)
66596100241ciprofloxacinyesyes
665966104kanamycinyesyes
665966472lincomycinyesyes
66596100246norfloxacinyesyes
6659617076streptomycinyesyes
6659628669bacitracinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
6659689634melaninyes
6659616136hydrogen sulfideno

enzymes

@refvalueactivityec
66596alpha-chymotrypsin-3.4.21.1
66596alpha-fucosidase-3.2.1.51
66596alpha-galactosidase-3.2.1.22
66596alpha-glucosidase-3.2.1.20
66596alpha-mannosidase-3.2.1.24
66596beta-galactosidase-3.2.1.23
66596beta-glucosidase-3.2.1.21
66596beta-glucuronidase-3.2.1.31
66596trypsin-3.4.21.4
66596acid phosphatase+3.1.3.2
66596alkaline phosphatase+3.1.3.1
66596catalase+1.11.1.6
66596cystine arylamidase+3.4.11.3
66596cytochrome oxidase+1.9.3.1
66596esterase+
66596esterase Lipase (C 8)+
66596leucine arylamidase+3.4.11.1
66596lipase+
66596N-acetyl-beta-glucosaminidase+3.2.1.52
66596naphthol-AS-BI-phosphohydrolase+
66596valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66596C15:1 w6c3
    66596C18:1 w9c0.6
    66596C17:0 anteiso0.9
    66596C18:0 iso0.9
    66596C16:0 10-methyl / iso-C17:1w9c1.9
    66596C17:1 w8c13.5
    66596C16:1 w7c / C16:1 w6c2.1
    66596C17:0 10-methyl2.1
    66596C17:0 iso2.3
    66596C16:02.6
    66596C17:1 w6c3.1
    66596C16:0 iso42.8
    66596C16:1 iso H5.7
    66596C17:06.6
    66596C15:0 iso8.1
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • incubation temperature: 25
  • incubation time: 5
  • library/peak naming table: TSBA 6.0
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

  • @ref: 66596
  • sample type: surface of an antarctic brown macroalga Adenocystis utricularis
  • host species: Adenocystis utricularis
  • sampling date: 2014-01-30
  • geographic location: Punta Rodriguez Site, King George Island
  • country: Antarctica
  • origin.country: ATA
  • continent: Australia and Oceania
  • latitude: -62.1992
  • longitude: -58.9428
  • enrichment culture: ZoBell's marine 2216E medium

isolation source categories

Cat1Cat2Cat3
#Host#Algae#Brown Algae
#Environmental#Aquatic#Marine
#Climate#Cold#Polar

Sequence information

16S sequences

  • @ref: 66596
  • description: Amycolatopsis sp. AU-G6 16S ribosomal RNA gene, partial sequence
  • accession: KX084450
  • length: 1421
  • database: ena
  • NCBI tax ID: 1854586

GC content

  • @ref: 66596
  • GC-content: 70.30
  • method: genome sequence analysis

External links

@ref: 66596

culture collection no.: CGMCC 4.7351, NBRC 112404

Reference

@idauthorstitledoi/urljournal
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
66596Jian Wang, Sergio Leiva, Jiao Huang and Ying HuangAmycolatopsis antarctica sp. nov., isolated from the surface of an Antarctic brown macroalga10.1099/ijsem.0.002844IJSEM 68: 2348-2356 2018