Name: Paenibacillus flagellatus DXL2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 166418

Type strain:

Species: Paenibacillus flagellatus

Strain Designation: DXL2

Culture col. no.: KCTC 33976, CCTCC AB 2018054

NCBI tax ID(s): 2211139 (species)

Section

Paenibacillus flagellatus DXL2 is an aerobe, spore-forming, Gram-negative bacterium that forms circular colonies and was isolated from selenium mineral soil sample.

colony-forming
Gram-negative
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus flagellatus**
	Full Scientific Name (LPSN)	Paenibacillus flagellatus Dai et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67784]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #67784]	Cell length	1.3-2.8 µm

[Ref.: #67784]	Cell width	0.4-0.8 µm

[Ref.: #67784]	Cell shape	rod-shaped

[Ref.: #67784]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.592

[Ref.: #67784]	Flagellum arrangement	peritrichous

[Ref.: #67784]	Colony size	1 mm
[Ref.: #67784]	Colony color	white
[Ref.: #67784]	Colony shape	circular
[Ref.: #67784]	Cultivation medium used	Reasoner's 2A agar (R2A)

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #67784]			growth	15-49 °C
[Ref.: #67784]			optimum	37 °C

[Ref.: #67784]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #67784]			optimum	7-8
[Ref.: #67784]			growth	5.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67784]

Oxygen tolerance

aerobe

[Ref.: #67784]	Type of spore	endospore
[Ref.: #67784]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67784]		NaCl		growth	0-1	%(w/v)
[Ref.: #67784]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67784]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #67784]	casein	+	hydrolysis
[Ref.: #67784]	cellobiose ChEBI	-	builds acid from
[Ref.: #67784]	D-fructose ChEBI	-	builds acid from
[Ref.: #67784]	D-galactose ChEBI	+	assimilation
[Ref.: #67784]	D-galactose ChEBI	-	builds acid from
[Ref.: #67784]	D-glucose ChEBI	+	assimilation
[Ref.: #67784]	D-glucose ChEBI	-	builds acid from
[Ref.: #67784]	D-mannitol ChEBI	-	assimilation
[Ref.: #67784]	D-mannose ChEBI	-	assimilation
[Ref.: #67784]	D-mannose ChEBI	-	builds acid from
[Ref.: #67784]	D-ribose ChEBI	+	assimilation
[Ref.: #67784]	D-ribose ChEBI	-	builds acid from
[Ref.: #67784]	D-xylose ChEBI	+	assimilation
[Ref.: #67784]	D-xylose ChEBI	-	builds acid from
[Ref.: #67784]	dna ChEBI	+	hydrolysis
[Ref.: #67784]	esculin ferric citrate	+	hydrolysis
[Ref.: #67784]	gelatin ChEBI	+	hydrolysis
[Ref.: #67784]	glucose ChEBI	-	fermentation
[Ref.: #67784]	L-arabinose ChEBI	-	assimilation
[Ref.: #67784]	L-arabinose ChEBI	-	builds acid from
[Ref.: #67784]	L-arginine ChEBI	+	hydrolysis
[Ref.: #67784]	L-proline ChEBI	+	assimilation
[Ref.: #67784]	L-sorbose ChEBI	-	builds acid from
[Ref.: #67784]	L-tryptophan ChEBI	-	assimilation
[Ref.: #67784]	lactose ChEBI	-	builds acid from
[Ref.: #67784]	malate ChEBI	-	assimilation
[Ref.: #67784]	maltose ChEBI	+	assimilation
[Ref.: #67784]	maltose ChEBI	-	builds acid from
[Ref.: #67784]	mannitol ChEBI	-	builds acid from
[Ref.: #67784]	myo-inositol ChEBI	-	builds acid from
[Ref.: #67784]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #67784]	nitrate ChEBI	-	reduction
[Ref.: #67784]	potassium gluconate ChEBI	-	assimilation
[Ref.: #67784]	raffinose ChEBI	+	builds acid from
[Ref.: #67784]	rhamnose ChEBI	-	builds acid from
[Ref.: #67784]	sorbitol ChEBI	-	builds acid from
[Ref.: #67784]	starch ChEBI	+	hydrolysis
[Ref.: #67784]	sucrose ChEBI	+	assimilation
[Ref.: #67784]	sucrose ChEBI	-	builds acid from
[Ref.: #67784]	trehalose ChEBI	-	builds acid from
[Ref.: #67784]	tween 40 ChEBI	+	hydrolysis
[Ref.: #67784]	tween 60 ChEBI	+	hydrolysis
[Ref.: #67784]	tween 80 ChEBI	+	hydrolysis
[Ref.: #67784]	urea ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #67784]	acetoin ChEBI	no
[Ref.: #67784]	hydrogen sulfide ChEBI	yes
[Ref.: #67784]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #67784]		acetoin ChEBI	-
[Ref.: #67784]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #67784]	acid phosphatase	+	3.1.3.2
[Ref.: #67784]	alkaline phosphatase	+	3.1.3.1
[Ref.: #67784]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #67784]	alpha-fucosidase	-	3.2.1.51
[Ref.: #67784]	alpha-galactosidase	+	3.2.1.22
[Ref.: #67784]	alpha-mannosidase	-	3.2.1.24
[Ref.: #67784]	beta-galactosidase	+	3.2.1.23
[Ref.: #67784]	beta-glucosidase	-	3.2.1.21
[Ref.: #67784]	beta-glucuronidase	-	3.2.1.31
[Ref.: #67784]	catalase	+	1.11.1.6
[Ref.: #67784]	cystine arylamidase	-	3.4.11.3
[Ref.: #67784]	cytochrome oxidase	-	1.9.3.1
[Ref.: #67784]	esterase (C 4)	+
[Ref.: #67784]	esterase Lipase (C 8)	+
[Ref.: #67784]	leucine arylamidase	+	3.4.11.1
[Ref.: #67784]	lipase (C 14)	-
[Ref.: #67784]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #67784]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #67784]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #67784]	trypsin	-	3.4.21.4
[Ref.: #67784]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #67784]

Fatty acid	Percentage
C15:0 anteiso	30
C16:0 iso	29.6
C15:0 iso	14.9
C17:0 anteiso	8.4
C17:0 iso	6.9
C16:0	5.6
C14:0 iso	2.7
C17:0	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67784]	Sample type/isolated from	selenium mineral soil sample
[Ref.: #67784]	Geographic location (country and/or sea, region)	selenium mine in Yutangba Village, Boyangba Town, Lichuan City, Hubei province
[Ref.: #67784]	Country	China
[Ref.: #67784]	Country ISO 3 Code	CHN
[Ref.: #67784]	Continent	Asia
[Ref.: #67784]	Geographic location	30.5441°/108.9360°
[Ref.: #67784]	Enrichment culture	R2A agar
[Ref.: #67784]	Enrichment culture duration	10 days
[Ref.: #67784]	Enrichment culture temperature	28 ̊C
[Ref.: #67784]	Isolation/enrichment procedure	dilution-plating method
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67784]	Paenibacillus sp. DXL2 16S ribosomal RNA gene, partial sequence	MH396718	1498	ENA	2211139 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67784]	Paenibacillus flagellatus DXL2	GCA_003217775	contig	GenBank	2211139 tax ID
[Ref.: #66792]	Paenibacillus sp. DXL2	2211139.3	wgs	PATRIC	2211139 tax ID	*
[Ref.: #66792]	Paenibacillus flagellatus DXL2	2882543331	draft	JGI IMG/M	2211139 tax ID	*

[Ref.: #67784]

GC-content

60.2 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paenibacillus sp. DXL2 PATRIC: 2211139.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus sp. DXL2 PATRIC: 2211139.3
[Ref.: #69480]	motile	yes	89.831	yes
[Ref.: #69480]	gram-positive	yes	64.776	yes
[Ref.: #69480]	anaerobic	no	98.328	yes
[Ref.: #69480]	aerobic	yes	87.238	yes
[Ref.: #69480]	halophile	no	94.749	yes
[Ref.: #69480]	spore-forming	yes	96.659	yes
[Ref.: #69480]	glucose-util	yes	89.765	yes
[Ref.: #69480]	flagellated	yes	85.072	yes
[Ref.: #69480]	thermophile	no	95.096	yes
[Ref.: #69480]	glucose-ferment	no	90.641	yes

Availability in culture collections External links

[Ref.: #67784]

Culture collection no.

KCTC 33976, CCTCC AB 2018054

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus flagellatus sp. nov., isolated from selenium mineral soil.	Dai X, Shi K, Wang X, Fan J, Wang R, Zheng S, Wang G	Int J Syst Evol Microbiol	10.1099/ijsem.0.003125	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67784

Xingli Dai, Kaixiang Shi, Xin Wang, Jun Fan, Rui Wang, Shixue Zheng, Gejiao Wang: Paenibacillus flagellatus sp. nov., isolated from selenium mineral soil. IJSEM 69: 183 - 188 2019 ( DOI 10.1099/ijsem.0.003125 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Paenibacillus sp. DXL2

Paenibacillus sp. DXL2

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