Name: Streptomyces otsuchiensis OTB305
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 166286

Type strain:

Species: Streptomyces otsuchiensis

Strain Designation: OTB305

Culture col. no.: NBRC 113255, TBRC 9682, DSM 114175

Strain history: <- TBRC; TBRC 9682 <- IFO; NBRC 113255

NCBI tax ID(s): 2681388 (species)

SI-ID 404724

DSM 114175

Special Collections

Section

Streptomyces otsuchiensis OTB305 is an aerobe, spore-forming, Gram-positive bacterium that has a light yellow pigmentation and was isolated from marine sediment.

Gram-positive
pigmented
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces otsuchiensis**
	Full Scientific Name (LPSN)	Streptomyces otsuchiensis Terahara et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #68033]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %90.316

[Ref.: #69480]	Motility	no Confidence in %90

[Ref.: #68033]	Pigment production	yes
[Ref.: #68033]	Pigmentation color	light yellow
[Ref.: #68033]	Pigment production	yes
[Ref.: #68033]	Pigmentation color	bright ash-yellow
[Ref.: #68033]	Pigment name	bright ash-yellow

Multimedia content

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Caption:

Medium871, 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM + SEAWATER (DSMZ Medium 871)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #68033]			growth	15-37 °C
[Ref.: #69125]			growth	28 °C

	pH	Kind of pH		pH
[Ref.: #68033]			growth	7.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68033]

Oxygen tolerance

aerobe

[Ref.: #68033]	Spore description	Rectiflexibile, spore chains are observed on aerial mycelia. The spores are 0.6-1.2 0.8-1.6 µm in size and spore surface is smooth.
[Ref.: #68033]	Type of spore	spore
[Ref.: #68033]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68033]		NaCl		growth	1.0-6.0	%(w/v)

[Ref.: #68033]	Observation	The peptidoglycan contains LL-diaminopimelic acid
[Ref.: #68033]	Observation	Whole-cell hydrolysates contain glucose and lack characteristic major sugars
[Ref.: #68033]	Observation	The predominant menaquinones are MK-9(H8) and MK-9(H6); MK-9(H4) and MK-9(H10) are minor components
[Ref.: #68033]	Observation	Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid are observed as the polar lipids
[Ref.: #68033]	Observation	colour of aerial mycelia are white to yellow-white
[Ref.: #68033]	Observation	Substrate mycelia appear light yellow on ISP1, ISP4 and ISP6, dark reddish yellow on ISP2 and ISP5, dark yellow red on ISP3, ash yellow red on ISP7, and bright reddish yellow on ISP8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68033]	D-fructose ChEBI	+	carbon source
[Ref.: #68033]	D-glucose ChEBI	+	carbon source
[Ref.: #68033]	D-mannitol ChEBI	+	carbon source
[Ref.: #68033]	D-xylose ChEBI	+	carbon source
[Ref.: #68033]	L-arabinose ChEBI	+	carbon source
[Ref.: #68033]	myo-inositol ChEBI	+	carbon source
[Ref.: #68033]	nitrate ChEBI	-	reduction
[Ref.: #68033]	raffinose ChEBI	+	carbon source
[Ref.: #68033]	rhamnose ChEBI	+	carbon source
[Ref.: #68033]	sucrose ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #68033]	acid phosphatase	+/-	3.1.3.2
[Ref.: #68033]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68033]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #68033]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68033]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68033]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68033]	alpha-mannosidase	+	3.2.1.24
[Ref.: #68033]	beta-galactosidase	-	3.2.1.23
[Ref.: #68033]	beta-glucosidase	-	3.2.1.21
[Ref.: #68033]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68033]	catalase	+	1.11.1.6
[Ref.: #68033]	cystine arylamidase	+/-	3.4.11.3
[Ref.: #68033]	esterase (C 4)	+/-
[Ref.: #68033]	esterase Lipase (C 8)	+/-
[Ref.: #68033]	gelatinase	+
[Ref.: #68033]	leucine arylamidase	+	3.4.11.1
[Ref.: #68033]	lipase (C 14)	+/-
[Ref.: #68033]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #68033]	naphthol-AS-BI-phosphohydrolase	+/-
[Ref.: #68033]	pyrazinamidase	+	3.5.1.B15
[Ref.: #68033]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #68033]	trypsin	-	3.4.21.4
[Ref.: #68033]	urease	-	3.5.1.5
[Ref.: #68033]	valine arylamidase	+/-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #68033]	Sample type/isolated from	marine sediment
[Ref.: #68033]	Geographic location (country and/or sea, region)	Otsuchi Bay, Iwate Prefecture
[Ref.: #68033]	Country	Japan
[Ref.: #68033]	Country ISO 3 Code	JPN
[Ref.: #68033]	Continent	Asia

[Ref.: #69125]	Sample type/isolated from	marine sediment
[Ref.: #69125]	Country	Japan
[Ref.: #69125]	Country ISO 3 Code	JPN
[Ref.: #69125]	Continent	Asia
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #68033]	Streptomyces otsuchiensis OTB305 gene for 16S ribosomal RNA, partial sequence	LC430327	1461	GenBank ENA	2681388 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68033]	Streptomyces otsuchiensis OTB305	GCA_004305975	scaffold	GenBank ENA	2681388 tax ID
[Ref.: #66792]	Streptomyces sp. OTB305	2484744.3	wgs	PATRIC	2681388 tax ID	*
[Ref.: #66792]	Streptomyces otsuchiensis OTB305	2868441088	draft	JGI IMG/M	2681388 tax ID	*

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GC-content

72.83 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptomyces otsuchiensis OTB305 NCBI: GCA_004305975.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptomyces sp. OTB305 PATRIC: 2484744.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.316	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.863	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	88.734	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	89.863	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	91.047	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90	no

Availability in culture collections External links

[Ref.: #68033]

Culture collection no.

NBRC 113255, TBRC 9682, DSM 114175

[Ref.: #114768]

SI-ID 404724

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces otsuchiensis sp. nov., a biosurfactant-producing actinobacterium isolated from marine sediment.	Terahara T, Naemura T, Nampo Y, Kobayashi T, Imada C, Hamada M, Tamura T	Int J Syst Evol Microbiol	10.1099/ijsem.0.003638	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68033

Takeshi Terahara, Takuya Naemura, Yukiko Nampo, Takeshi Kobayashi, Chiaki Imada, Moriyuki Hamada, Tomohiko Tamura: Streptomyces otsuchiensis sp. nov., a biosurfactant-producing actinobacterium isolated from marine sediment. IJSEM 69: 3740 - 3744 2019 ( DOI 10.1099/ijsem.0.003638 )

#69125

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 114175

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#114768

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404724.1 )

* These data were automatically processed and therefore are not curated

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Streptomyces otsuchiensis OTB305

Streptomyces sp. OTB305

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