Name: Chryseobacterium phosphatilyticum ISE14
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164405

Type strain:

Species: Chryseobacterium phosphatilyticum

Strain Designation: ISE14

Culture col. no.: JCM 32876, KACC 19820

Strain history: K. D. Kim; Korea Univ., South Korea; ISE14.

NCBI tax ID(s): 475075 (species)

Section

Chryseobacterium phosphatilyticum ISE14 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from Sterilized root of a cucumber plant collected in a field in Iksan.

colony-forming
Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Weeksellaceae*
	Genus	*Chryseobacterium*
	Species	**Chryseobacterium phosphatilyticum**
	Full Scientific Name (LPSN)	Chryseobacterium phosphatilyticum Jeong et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67779]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.989

[Ref.: #67779]	Cell length	1.5-2.2 µm

[Ref.: #67779]	Cell width	0.5-0.9 µm

[Ref.: #67779]	Cell shape	rod-shaped

[Ref.: #67779]	Motility	no

[Ref.: #67779]	Colony color	yellow
[Ref.: #67779]	Colony shape	circular
[Ref.: #67779]	Cultivation medium used	Nutrient agar (NA)

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	30 °C
[Ref.: #67779]	growth	15-28 °C
[Ref.: #67779]	optimum	25-28 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67779]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #67779]			optimum	7
[Ref.: #67779]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67779]

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998
[Ref.: #69481]	Ability of spore formation	no Confidence in %99

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67779]		NaCl		growth	1-4	%
[Ref.: #67779]		NaCl		optimum	1-2	%

[Ref.: #67770]

Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67779]	amygdalin ChEBI	-	builds acid from
[Ref.: #67779]	casein	+	hydrolysis
[Ref.: #67779]	cellobiose ChEBI	-	builds acid from
[Ref.: #67779]	citrate ChEBI	+	assimilation
[Ref.: #67779]	D-fructose ChEBI	-	builds acid from
[Ref.: #67779]	D-glucose ChEBI	-	builds acid from
[Ref.: #67779]	D-mannitol ChEBI	-	builds acid from
[Ref.: #67779]	D-mannose ChEBI	-	builds acid from
[Ref.: #67779]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #67779]	esculin ChEBI	+	degradation
[Ref.: #67779]	L-arabinose ChEBI	-	builds acid from
[Ref.: #67779]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #67779]	maltose ChEBI	+	builds acid from
[Ref.: #67779]	melibiose ChEBI	-	builds acid from
[Ref.: #67779]	myo-inositol ChEBI	-	builds acid from
[Ref.: #67779]	nitrate ChEBI	-	reduction
[Ref.: #67779]	starch ChEBI	+	hydrolysis
[Ref.: #67779]	sucrose ChEBI	-	builds acid from
[Ref.: #67779]	trehalose ChEBI	-	builds acid from
[Ref.: #67779]	tween 20 ChEBI	+	hydrolysis
[Ref.: #67779]	tween 80 ChEBI	+	hydrolysis
[Ref.: #67779]	tyrosine ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #67779]	acetoin ChEBI	no
[Ref.: #67779]	hydrogen sulfide ChEBI	no
[Ref.: #67779]	indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #67779]	acid phosphatase	+	3.1.3.2
[Ref.: #67779]	alkaline phosphatase	+	3.1.3.1
[Ref.: #67779]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #67779]	alpha-fucosidase	-	3.2.1.51
[Ref.: #67779]	alpha-galactosidase	-	3.2.1.22
[Ref.: #67779]	alpha-glucosidase	-	3.2.1.20
[Ref.: #67779]	alpha-mannosidase	-	3.2.1.24
[Ref.: #67779]	arginine dihydrolase	-	3.5.3.6
[Ref.: #67779]	beta-galactosidase	-	3.2.1.23
[Ref.: #67779]	beta-glucosidase	-	3.2.1.21
[Ref.: #67779]	beta-glucuronidase	-	3.2.1.31
[Ref.: #67779]	catalase	+	1.11.1.6
[Ref.: #67779]	cystine arylamidase	-	3.4.11.3
[Ref.: #67779]	cytochrome oxidase	+	1.9.3.1
[Ref.: #67779]	esterase (C 4)	+
[Ref.: #67779]	esterase Lipase (C 8)	+
[Ref.: #67779]	gelatinase	+
[Ref.: #67779]	leucine arylamidase	+	3.4.11.1
[Ref.: #67779]	lipase (C 14)	-
[Ref.: #67779]	lysine decarboxylase	-	4.1.1.18
[Ref.: #67779]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #67779]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #67779]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #67779]	trypsin	-	3.4.21.4
[Ref.: #67779]	tryptophan deaminase	-	4.1.99.1
[Ref.: #67779]	urease	+	3.5.1.5
[Ref.: #67779]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Sterilized root of a cucumber (Cucumis sativus L.) plant collected in a field in Iksan
[Ref.: #67770]	Host species	Cucumis sativus
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jeonbuk Province
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67779]	Sample type/isolated from	root of a cucumber (Cucumis sativus L.) plant
[Ref.: #67779]	Sampling date	2002
[Ref.: #67779]	Geographic location (country and/or sea, region)	Iksan, Jeonbuk province
[Ref.: #67779]	Country	Republic of Korea
[Ref.: #67779]	Country ISO 3 Code	KOR
[Ref.: #67779]	Continent	Asia
[Ref.: #67779]	Geographic location	35.9438°/126.9540°
[Ref.: #67779]	Enrichment culture	tryptic soy agar
[Ref.: #67779]	Enrichment culture composition	supplemented with 50 µg ml-1 cycloheximide
[Ref.: #67779]	Enrichment culture duration	48 h
[Ref.: #67779]	Enrichment culture temperature	28 ̊C
[Ref.: #67779]	Isolation/enrichment procedure	the root sample was surfaced-sterilized with 1 % sodium hypochlorite for 90 s, washed several times with sterile distilled water and ground with a sterile homogenizer. This ground root was placed in 100 ml of sterile distilled water and kept in a rotary shaker (160 r.p.m.) at 28°C for 30 min. Then, the suspension was spread on tryptic soy agar and further incubated at the same temperature for 48 h.
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Chryseobacterium phosphatilyticum 16S ribosomal RNA gene, complete sequence	EU034659	1442	ENA	475075 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Chryseobacterium phosphatilyticum ISE14	GCA_002899825	scaffold	GenBank	475075 tax ID
[Ref.: #66792]	Chryseobacterium phosphatilyticum ISE14	2874358511	draft	JGI IMG/M	475075 tax ID	*

[Ref.: #67770]

GC-content

36.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Chryseobacterium phosphatilyticum ISE14 NCBI: GCA_002899825.2
[Ref.: #69481]	spore-forming	no	99	no

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 32876, KACC 19820

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Chryseobacterium phosphatilyticum sp. nov., a phosphate-solubilizing endophyte isolated from cucumber (Cucumis sativus L.) root.	Jeong JJ, Sang MK, Lee DW, Choi IG, Kim KD	Int J Syst Evol Microbiol	10.1099/ijsem.0.003091	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#67779

Jin-Ju Jeong, Mee Kyung Sang, Dong Wan Lee, In-Geol Choi, Ki Deok Kim: Chryseobacterium phosphatilyticum sp. nov., a phosphate-solubilizing endophyte isolated from cucumber (Cucumis sativus L.) root. IJSEM 69: 610 - 615 2019 ( DOI 10.1099/ijsem.0.003091 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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