Name: Ephemeroptericola cinctiostellae F02
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164343

Type strain:

Species: Ephemeroptericola cinctiostellae

Strain Designation: F02

Culture col. no.: JCM 32722, FBCC 500047, KCTC 62567

Strain history: H. Kim; Nakdonggang Natl. Inst. of Biol. Resour., South Korea; F02.

NCBI tax ID(s): 2268024 (species)

Section

Ephemeroptericola cinctiostellae F02 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from Gut of the insect Cincticostella levanidovae collected in the Sangju district.

colony-forming
Gram-negative
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Ephemeroptericola*
	Species	**Ephemeroptericola cinctiostellae**
	Full Scientific Name (LPSN)	Ephemeroptericola cinctiostellae Kim et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67851]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.95

[Ref.: #67851]	Cell length	0.7-2.8 µm

[Ref.: #67851]	Cell width	0.4-0.5 µm

[Ref.: #67851]	Cell shape	rod-shaped

[Ref.: #67851]	Motility	no

[Ref.: #67851]	Colony size	1-1.5 mm
[Ref.: #67851]	Colony shape	circular
[Ref.: #67851]	Incubation period	3 days
[Ref.: #67851]	Cultivation medium used	Reasoner's 2A agar (R2A)

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	25 °C
[Ref.: #67851]	growth	4-30 °C
[Ref.: #67851]	optimum	25 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67851]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #67851]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67851]

Oxygen tolerance

facultative anaerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %100
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67851]		NaCl		growth	0-0.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67851]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #67851]	casein	-	hydrolysis
[Ref.: #67851]	D-glucose ChEBI	+	assimilation
[Ref.: #67851]	esculin ChEBI	+	hydrolysis
[Ref.: #67851]	malate ChEBI	+	assimilation
[Ref.: #67851]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #67851]	nitrate ChEBI	-	reduction
[Ref.: #67851]	starch ChEBI	-	hydrolysis
[Ref.: #67851]	tween 20 ChEBI	-	hydrolysis

	Metabolite	Production
[Ref.: #67851]	acetoin ChEBI	no
[Ref.: #67851]	hydrogen sulfide ChEBI	no
[Ref.: #67851]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #67851]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #67851]	acid phosphatase	+	3.1.3.2
[Ref.: #67851]	alkaline phosphatase	-	3.1.3.1
[Ref.: #67851]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #67851]	alpha-fucosidase	-	3.2.1.51
[Ref.: #67851]	alpha-galactosidase	-	3.2.1.22
[Ref.: #67851]	alpha-glucosidase	+	3.2.1.20
[Ref.: #67851]	alpha-mannosidase	-	3.2.1.24
[Ref.: #67851]	beta-galactosidase	+	3.2.1.23
[Ref.: #67851]	beta-glucosidase	+	3.2.1.21
[Ref.: #67851]	beta-glucuronidase	-	3.2.1.31
[Ref.: #67851]	catalase	+	1.11.1.6
[Ref.: #67851]	cystine arylamidase	-	3.4.11.3
[Ref.: #67851]	cytochrome oxidase	+	1.9.3.1
[Ref.: #67851]	esterase (C 4)	+
[Ref.: #67851]	esterase Lipase (C 8)	+
[Ref.: #67851]	leucine arylamidase	+	3.4.11.1
[Ref.: #67851]	lipase (C 14)	-
[Ref.: #67851]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #67851]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #67851]	trypsin	-	3.4.21.4
[Ref.: #67851]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Gut of the insect Cincticostella levanidovae (Tshernova) collected in the Sangju district
[Ref.: #67770]	Host species	Cincticostella levanidovae
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67851]	Sample type/isolated from	gut of C. levanidovae
[Ref.: #67851]	Geographic location (country and/or sea, region)	Sangju district
[Ref.: #67851]	Country	Republic of Korea
[Ref.: #67851]	Country ISO 3 Code	KOR
[Ref.: #67851]	Continent	Asia
[Ref.: #67851]	Geographic location	36.3222°/128.1640°
[Ref.: #67851]	Enrichment culture	R2A agar
[Ref.: #67851]	Enrichment culture temperature	25 ̊C
[Ref.: #67851]	Isolation/enrichment procedure	The sample was diluted and plated on Reasoner's 2A agar using standard techniques and incubated at 25°C.
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ephemeroptericola cinctiostellae strain F02 16S ribosomal RNA gene, partial sequence	MH136843	1512	ENA	2268024 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67851]	Ephemeroptericola cinctiostellae F02	GCA_003339525	complete	GenBank	2268024 tax ID
[Ref.: #66792]	Ephemeroptericola cinctiostellae F02	2848339753	complete	JGI IMG/M	2268024 tax ID	*

[Ref.: #67851]

GC-content

48.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ephemeroptericola cinctiostellae F02 NCBI: GCA_003339525.1
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 32722, FBCC 500047, KCTC 62567

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ephemeroptericola cinctiostellae gen. nov., sp. nov., isolated from gut of an aquatic insect.	Kim H, Bae MJ, Yu SM, Oh J, Lee CS, Yeon Jo B, Chae JC, Kim JH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003296	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#67851

Hyangmi Kim, Mi-jung Bae, Sang Mi Yu, Jeongsu Oh, Chang Soo Lee, Bok Yeon Jo, Jong-Chan Chae, Jee-Hwan Kim: Ephemeroptericola cinctiostellae gen. nov., sp. nov., isolated from gut of an aquatic insect. IJSEM 69: 1546 - 1550 2019 ( DOI 10.1099/ijsem.0.003296 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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