Name: Labilibaculum euxinus A4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164274

Type strain:

Species: Labilibaculum euxinus

Strain Designation: A4

Culture col. no.: JCM 32481, KCTC 15662

Strain history: L. Villanueva; R. Neth. Inst. for Sea Res., Netherlands; A4.

NCBI tax ID(s): 2686357 (species)

Section

Labilibaculum euxinus A4 is a facultative anaerobe, chemoorganoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from Deep sulfidic water from the Black Sea.

colony-forming
Gram-negative
motile
rod-shaped
chemoorganoheterotroph
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Marinifilaceae*
	Genus	*Labilibaculum*
	Species	"Labilibaculum euxinus"
	Full Scientific Name (LPSN)	Labilibaculum euxinus Yadav et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #69631]	Gram stain	negative

[Ref.: #69631]	Cell length	3-6 µm

[Ref.: #69631]	Cell width	0.2-0.3 µm

[Ref.: #69631]	Cell shape	rod-shaped

[Ref.: #69631]	Motility	yes

[Ref.: #69631]	Colony color	light pink
[Ref.: #69631]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	28 °C
[Ref.: #69631]	growth	4-35 °C
[Ref.: #69631]	optimum	20-28 °C

[Ref.: #67770]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #69631]			optimum	7-8
[Ref.: #69631]			growth	6.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69631]

Oxygen tolerance

facultative anaerobe

[Ref.: #69631]

Nutrition type

chemoorganoheterotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69631]		NaCl		growth	0.5-5.5	%(w/v)
[Ref.: #69631]		NaCl		optimum	2-3	%(w/v)

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #69631]	Observation	Growth at elevated hydrostatic pressure (~50 MPa)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69631]	acetate ChEBI	+	assimilation
[Ref.: #69631]	cellobiose ChEBI	+	assimilation
[Ref.: #69631]	cellulose ChEBI	+	assimilation
[Ref.: #69631]	D-glucose ChEBI	+	assimilation
[Ref.: #69631]	D-mannitol ChEBI	+	assimilation
[Ref.: #69631]	D-mannose ChEBI	+	assimilation
[Ref.: #69631]	D-sorbitol ChEBI	-	growth
[Ref.: #69631]	D-trehalose ChEBI	-	growth
[Ref.: #69631]	D-xylose ChEBI	+	assimilation
[Ref.: #69631]	esculin ChEBI	+	hydrolysis
[Ref.: #69631]	L-arabinose ChEBI	+	assimilation
[Ref.: #69631]	L-aspartate ChEBI	+	assimilation
[Ref.: #69631]	L-glutamate ChEBI	+	assimilation
[Ref.: #69631]	L-lysine ChEBI	+	assimilation
[Ref.: #69631]	L-rhamnose ChEBI	-	growth
[Ref.: #69631]	lactose ChEBI	+	assimilation
[Ref.: #69631]	maltose ChEBI	+	assimilation
[Ref.: #69631]	melezitose ChEBI	-	growth
[Ref.: #69631]	nitrate ChEBI	-	reduction
[Ref.: #69631]	raffinose ChEBI	-	growth
[Ref.: #69631]	salicin ChEBI	+	assimilation
[Ref.: #69631]	starch ChEBI	+	assimilation
[Ref.: #69631]	sucrose ChEBI	+	assimilation
[Ref.: #69631]	yeast extract	+	required for growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #69631]		hydrogen sulfide ChEBI	no
[Ref.: #69631]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #69631]	amylase	+
[Ref.: #69631]	catalase	-	1.11.1.6
[Ref.: #69631]	cellulase	+	3.2.1.4
[Ref.: #69631]	chitinase	-	3.2.1.14
[Ref.: #69631]	cytochrome oxidase	-	1.9.3.1
[Ref.: #69631]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #69631]

Fatty acid	Percentage
iso-C15:0	45.5
iso-C17:0 3OH	18.3
iso-C15:0 3OH	8.7
anteiso-C15:0	7.7
iso-C15:1 w8c	3.5
anteiso-C17:0 3OH	3.4
C16:0	2.2
C15:0	1.7
iso-C17:1 w8c	1.5
C16:0 3OH	1.5
iso-C17:0	0.9
anteiso-C15:0 3OH	0.7
C16:1 w7c	0.5
anteiso-C15:1 w8c	0.5
iso-C17:1 w8trans	0.5
iso-C13:0	0.4
iso-C16:0	0.3
iso-C17:0 amide 3OH Glycine	0.3
anteiso-C17:0	0.2
iso-C15:1 w8c 3OH	0.1
C14:0	0.1
iso-C17:1 w6c	0.1
anteiso-C13:0	0.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Deep sulfidic water (2,000m depth) from the Black Sea

[Ref.: #69631]	Sample type/isolated from	Sulfidic waters of the Black Sea
[Ref.: #69631]	Geographic location (country and/or sea, region)	Black Sea
[Ref.: #69631]	Country	Bulgaria
[Ref.: #69631]	Country ISO 3 Code	BGR
[Ref.: #69631]	Continent	Europe
[Ref.: #69631]	Geographic location	42.8833°/30.6667°
[Ref.: #69631]	Enrichment culture composition	see materials and methods section of paper
[Ref.: #69631]	Enrichment culture duration	10 days
[Ref.: #69631]	Enrichment culture temperature	10 and 20 ̊C
[Ref.: #69631]	Isolation date	2017-11-22
[Ref.: #69631]	Isolation/enrichment procedure	grown in anaerobic jars
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ancylomarina sp. A4 partial 16S rRNA gene, strain A4	LS999417	1526	ENA	2686357 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Labilibaculum euxinus A4	GCA_009795715	contig	GenBank	2686357 tax ID
[Ref.: #69631]	Whole genome shotgun	WTCR00000000		GenBank	2686357 tax ID

[Ref.: #67770]

GC-content

36.2 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Labilibaculum euxinus A4 NCBI: GCA_009795715.1
[Ref.: #69480]	motile	no	83.338	no
[Ref.: #69480]	flagellated	no	93.619	no
[Ref.: #69480]	gram-positive	no	97.326	no
[Ref.: #69480]	anaerobic	no	68.038	no
[Ref.: #69480]	aerobic	no	92.118	no
[Ref.: #69480]	halophile	no	72.241	no
[Ref.: #69480]	spore-forming	no	91.657	no
[Ref.: #69480]	glucose-util	yes	89.857	no
[Ref.: #69480]	thermophile	no	98.67	no
[Ref.: #69480]	glucose-ferment	no	55.416	no

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 32481, KCTC 15662

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69631

Subhash Yadav, Laura Villanueva, Nicole Bale, Michel Koenen, Ellen C. Hopmans, Jaap S. Sinninghe Damsté: Physiological, chemotaxonomic and genomic characterization of two novel piezotolerant bacteria of the family Marinifilaceae isolated from sulfidic waters of the Black Sea. Syst Appl Microbiol. 43: 2020 ( DOI 10.1016/j.syapm.2020.126122 )

* These data were automatically processed and therefore are not curated

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