Marinifilum breve (KCTC 15646, MCCC 1K03477, JCM 32401)

Name: Marinifilum breve (KCTC 15646, MCCC 1K03477, JCM 32401)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 159845

Type strain

Strain Designation JC075

Culture col. no. KCTC 15646 MCCC 1K03477 JCM 32401 JC075 MCC 1K03477

NCBI tax ID(s) 2184082

Special Collections JCM KCTC Literature strains

Links

Marinifilum breve JC075 is a facultative anaerobe, psychrophilic, Gram-negative prokaryote that forms circular colonies and was isolated from From Sansha Yongle Blue Hole of Paracel Islands in the South China Sea at a depth of 90 m.

Gram-negative rod-shaped colony-forming facultative anaerobe psychrophilic genome sequence

Name and taxonomic classification

SI-ID 27778

KCTC 15646,JCM 32401

@ref 20215

Last LPSN update 2025-12-16 10:08:21

Domain Pseudomonadati

Phylum Bacteroidota

Class Bacteroidia

Order Bacteroidales

Family Marinifilaceae

Genus Marinifilum

Species Marinifilum breve

Full scientific name Marinifilum breve Fu et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
66740	negative	1.0-4.0 µm	0.3-0.6 µm	rod-shaped
67771				rod-shaped
67771	negative

Colony morphology

@ref	Colony color	Colony shape	Medium used
66740	beige	circular	Marine agar (MA)

Culture and growth conditions

Culture medium

@ref	Name	Growth
66740	Marine agar (MA)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
66740	positive	optimum	16	psychrophilic
66740	positive	growth	10-37
67770	positive	growth	16	psychrophilic
67771	positive	growth	16	psychrophilic

Culture pH

@ref	Ability	Type	PH	PH range
66740	positive	optimum	7
66740	positive	growth	6.0-9.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
66740	facultative anaerobe
67771	facultative anaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66740	NaCl	positive	growth	0-6.0 %(w/v)
66740	NaCl	positive	optimum	3 %(w/v)

Observation

@ref	Observation
67770	quinones: MK-7
67771	quinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66740	17256 ChEBI	2-deoxyadenosine	+	assimilation
66740	64552 ChEBI	2-hydroxybutyrate	+	assimilation
66740	58187 ChEBI	alginate	-	hydrolysis
66740	40585 ChEBI	alpha-cyclodextrin	+	assimilation
66740	17925 ChEBI	alpha-D-glucose	+	assimilation
66740	22599 ChEBI	arabinose	+	builds acid from
66740	495055 ChEBI	beta-cyclodextrin	+	assimilation
66740		casein	+	hydrolysis
66740	17057 ChEBI	cellobiose	+	builds acid from
66740	17029 ChEBI	chitin	-	hydrolysis
66740	15824 ChEBI	D-fructose	+	assimilation
66740	12936 ChEBI	D-galactose	+	assimilation
66740	18024 ChEBI	D-galacturonic acid	+	assimilation
66740	30612 ChEBI	D-glucarate	+	assimilation
66740	8391 ChEBI	D-gluconate	+	assimilation
66740	17784 ChEBI	D-glucosaminic acid	+	assimilation
66740	14314 ChEBI	D-glucose 6-phosphate	+	assimilation
66740	16024 ChEBI	D-mannose	+	assimilation
66740	17924 ChEBI	D-sorbitol	+	builds acid from
66740	23652 ChEBI	dextrin	+	assimilation
66740	16991 ChEBI	dna	+	hydrolysis
66740	4853 ChEBI	esculin	+	builds acid from
66740	15740 ChEBI	formate	+	assimilation
66740	16813 ChEBI	galactitol	+	assimilation
66740	5291 ChEBI	gelatin	+	builds acid from
66740	17234 ChEBI	glucose	+	builds acid from
66740	29042 ChEBI	glucose 1-phosphate	+	assimilation
66740	29987 ChEBI	glutamate	+	assimilation
66740	17754 ChEBI	glycerol	+	assimilation
66740	14336 ChEBI	glycerol 1-phosphate	+	assimilation
66740	17596 ChEBI	inosine	+	assimilation
66740	18287 ChEBI	L-fucose	+	assimilation
66740	18050 ChEBI	L-glutamine	+	assimilation
66740	17115 ChEBI	L-serine	+	assimilation
66740	16857 ChEBI	L-threonine	+	assimilation
66740	24996 ChEBI	lactate	+	assimilation
66740	17716 ChEBI	lactose	+	assimilation
66740	17716 ChEBI	lactose	+	builds acid from
66740	6359 ChEBI	lactulose	+	assimilation
66740	17306 ChEBI	maltose	+	builds acid from
66740	29864 ChEBI	mannitol	+	builds acid from
66740	37684 ChEBI	mannose	+	builds acid from
66740	28053 ChEBI	melibiose	+	assimilation
66740	55507 ChEBI	methyl alpha-D-galactoside	+	assimilation
66740	17540 ChEBI	methyl beta-D-galactoside	+	assimilation
66740	37657 ChEBI	methyl D-glucoside	+	assimilation
66740	51850 ChEBI	methyl pyruvate	+	assimilation
66740	63154 ChEBI	N-acetyl-beta-D-mannosamine	+	assimilation
66740	28800 ChEBI	N-acetylgalactosamine	+	assimilation
66740	59640 ChEBI	N-acetylglucosamine	+	assimilation
66740	15361 ChEBI	pyruvate	+	assimilation
66740	16634 ChEBI	raffinose	+	assimilation
66740	26546 ChEBI	rhamnose	+	builds acid from
66740	17814 ChEBI	salicin	+	builds acid from
66740	28017 ChEBI	starch	+	hydrolysis
66740	30031 ChEBI	succinate	+	assimilation
66740	17992 ChEBI	sucrose	+	assimilation
66740	17992 ChEBI	sucrose	+	builds acid from
66740	17748 ChEBI	thymidine	+	assimilation
66740	63528 ChEBI	thymidine 5'-monophosphate	+	assimilation
66740	27082 ChEBI	trehalose	+	assimilation
66740	27082 ChEBI	trehalose	+	builds acid from
66740	53424 ChEBI	tween 20	-	hydrolysis
66740	53423 ChEBI	tween 40	-	hydrolysis
66740	53426 ChEBI	tween 80	-	hydrolysis
66740	16704 ChEBI	uridine	+	assimilation
66740	16695 ChEBI	uridine 5'-monophosphate	+	assimilation
66740	18222 ChEBI	xylose	+	builds acid from

Enzymes

@ref	Value	Activity	Ec
66740	catalase	-	1.11.1.6
66740	cytochrome oxidase	-	1.9.3.1

Isolation, sampling and environmental information

Isolation

@ref	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure	Sample type
67771		China	CHN	Asia								From Sansha Yongle Blue Hole of Paracel Islands in the South China Sea at a depth of 90 m
66740	Yongle Blue Hole in the South China Sea	China	CHN	Asia	16.525	111.768 16.525/111.768	sterile fluid enrichment medium	per litre: 5 g peptone, 1 g yeast extract, 0.01 g FePO 4 , 0.3 g cysteine and 0.001 g resazurin	1 month	16	Strain JC075 was isolated from the Yongle Blue Hole in the South China Sea at water depth of 90 m in the water column. The seawater samples were quickly inoculated into a sterile fluid enrichment medium. The medium (per litre: 5 g peptone, 1 g yeast extract, 0.01 g FePO 4 , 0.3 g cysteine and 0.001 g resazurin) was prepared anaerobically and kept under an atmosphere of mixed gas (10 % H2 , 20 % CO2 and 70 % N2 ). Enrichment cultures were incubated at 16°C in 10 ml vials sealed with butyl-rubber stoppers. After 1 month, the bacterial liquid was coated on marine agar 2216 in an anaerobic packet of AneroPack-Anaero for bacterial purification.
67770												Younle Blue Hole in the South China Sea at a depth of 90 m

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67771	ASM320215v1 assembly for Marinifilum breve JC075	contig	GCA_003202155	2184082.3	2832567879	2184082	32.92

GC content

@ref	GC-content (mol%)	Method
67770	35.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Marinifilum breve JC075
NCBI: GCA_003202155

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	55.80	no
125439	motility	BacteriaNetⓘ	yes	50.80	no
125439	gram_stain	BacteriaNetⓘ	negative	78.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	83.90	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinifilum breve sp. nov., a marine bacterium isolated from the Yongle Blue Hole in the South China Sea and emended description of the genus Marinifilum.	Fu T, Jia C, Fu L, Zhou S, Yao P, Du R, Sun H, Yang Z, Shi X, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003027	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66740	Tianyu Fu, Chao Jia, Liang Fu, Shun Zhou, Peng Yao, Rui Du, Hao Sun, Zuosheng Yang, Xiaochong Shi, Xiao-Hua Zhang: Marinifilum breve sp. nov., a marine bacterium isolated from the Yongle Blue Hole in the South China Sea and emended description of the genus Marinifilum. IJSEM 68: 3540 - 3545 2018 ( DOI 10.1099/ijsem.0.003027 , PubMed 30230441 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Marinifilum breve (KCTC 15646, MCCC 1K03477, JCM 32401)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003202155

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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