Name: Marinifilum breve JC075
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159845

Type strain:

Species: Marinifilum breve

Strain Designation: JC075

Culture col. no.: KCTC 15646, MCCC 1K03477, JCM 32401, MCC 1K03477

Strain history: <- Xiaochong Shi, Ocean Univ. of China

NCBI tax ID(s): 2184082 (species)

Section

Marinifilum breve JC075 is a facultative anaerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from From Sansha Yongle Blue Hole of Paracel Islands in the South China Sea at a depth of 90 m.

colony-forming
Gram-negative
rod-shaped
facultative anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Marinifilaceae*
	Genus	*Marinifilum*
	Species	**Marinifilum breve**
	Full Scientific Name (LPSN)	Marinifilum breve Fu et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66740]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #66740]	Cell length	1.0-4.0 µm

[Ref.: #66740]	Cell width	0.3-0.6 µm

[Ref.: #66740]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #66740]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #66740]	Colony color	beige
[Ref.: #66740]	Colony shape	circular
[Ref.: #66740]	Cultivation medium used	Marine agar (MA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66740]

Culture medium

Marine agar (MA)

[Ref.: #66740]

Culture medium growth

		Temperature
[Ref.: #66740]	optimum	16 °C
[Ref.: #66740]	growth	10-37 °C
[Ref.: #67770]	growth	16 °C
[Ref.: #67771]	growth	16 °C

[Ref.: #66740]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	psychrophilic
[Ref.: #67771]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #66740]			optimum	7
[Ref.: #66740]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66740]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66740]		NaCl		growth	0-6.0	%(w/v)
[Ref.: #66740]		NaCl		optimum	3	%(w/v)

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66740]	2-deoxyadenosine ChEBI	+	assimilation
[Ref.: #66740]	2-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #66740]	alginate ChEBI	-	hydrolysis
[Ref.: #66740]	alpha-cyclodextrin ChEBI	+	assimilation
[Ref.: #66740]	alpha-D-glucose ChEBI	+	assimilation
[Ref.: #66740]	arabinose ChEBI	+	builds acid from
[Ref.: #66740]	beta-cyclodextrin ChEBI	+	assimilation
[Ref.: #66740]	casein	+	hydrolysis
[Ref.: #66740]	cellobiose ChEBI	+	builds acid from
[Ref.: #66740]	chitin ChEBI	-	hydrolysis
[Ref.: #66740]	D-fructose ChEBI	+	assimilation
[Ref.: #66740]	D-galactose ChEBI	+	assimilation
[Ref.: #66740]	D-galacturonic acid ChEBI	+	assimilation
[Ref.: #66740]	D-glucarate ChEBI	+	assimilation
[Ref.: #66740]	D-gluconate ChEBI	+	assimilation
[Ref.: #66740]	D-glucosaminic acid ChEBI	+	assimilation
[Ref.: #66740]	D-glucose 6-phosphate ChEBI	+	assimilation
[Ref.: #66740]	D-mannose ChEBI	+	assimilation
[Ref.: #66740]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #66740]	dextrin ChEBI	+	assimilation
[Ref.: #66740]	dna ChEBI	+	hydrolysis
[Ref.: #66740]	esculin ChEBI	+	builds acid from
[Ref.: #66740]	formate ChEBI	+	assimilation
[Ref.: #66740]	galactitol ChEBI	+	assimilation
[Ref.: #66740]	gelatin ChEBI	+	builds acid from
[Ref.: #66740]	glucose ChEBI	+	builds acid from
[Ref.: #66740]	glucose 1-phosphate ChEBI	+	assimilation
[Ref.: #66740]	glutamate ChEBI	+	assimilation
[Ref.: #66740]	glycerol ChEBI	+	assimilation
[Ref.: #66740]	glycerol 1-phosphate ChEBI	+	assimilation
[Ref.: #66740]	inosine ChEBI	+	assimilation
[Ref.: #66740]	L-fucose ChEBI	+	assimilation
[Ref.: #66740]	L-glutamine ChEBI	+	assimilation
[Ref.: #66740]	L-serine ChEBI	+	assimilation
[Ref.: #66740]	L-threonine ChEBI	+	assimilation
[Ref.: #66740]	lactate ChEBI	+	assimilation
[Ref.: #66740]	lactose ChEBI	+	assimilation
[Ref.: #66740]	lactose ChEBI	+	builds acid from
[Ref.: #66740]	lactulose ChEBI	+	assimilation
[Ref.: #66740]	maltose ChEBI	+	builds acid from
[Ref.: #66740]	mannitol ChEBI	+	builds acid from
[Ref.: #66740]	mannose ChEBI	+	builds acid from
[Ref.: #66740]	melibiose ChEBI	+	assimilation
[Ref.: #66740]	methyl alpha-D-galactoside ChEBI	+	assimilation
[Ref.: #66740]	methyl beta-D-galactoside ChEBI	+	assimilation
[Ref.: #66740]	methyl D-glucoside ChEBI	+	assimilation
[Ref.: #66740]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #66740]	N-acetyl-beta-D-mannosamine ChEBI	+	assimilation
[Ref.: #66740]	N-acetylgalactosamine ChEBI	+	assimilation
[Ref.: #66740]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #66740]	pyruvate ChEBI	+	assimilation
[Ref.: #66740]	raffinose ChEBI	+	assimilation
[Ref.: #66740]	rhamnose ChEBI	+	builds acid from
[Ref.: #66740]	salicin ChEBI	+	builds acid from
[Ref.: #66740]	starch ChEBI	+	hydrolysis
[Ref.: #66740]	succinate ChEBI	+	assimilation
[Ref.: #66740]	sucrose ChEBI	+	assimilation
[Ref.: #66740]	sucrose ChEBI	+	builds acid from
[Ref.: #66740]	thymidine ChEBI	+	assimilation
[Ref.: #66740]	thymidine 5'-monophosphate ChEBI	+	assimilation
[Ref.: #66740]	trehalose ChEBI	+	assimilation
[Ref.: #66740]	trehalose ChEBI	+	builds acid from
[Ref.: #66740]	tween 20 ChEBI	-	hydrolysis
[Ref.: #66740]	tween 40 ChEBI	-	hydrolysis
[Ref.: #66740]	tween 80 ChEBI	-	hydrolysis
[Ref.: #66740]	uridine ChEBI	+	assimilation
[Ref.: #66740]	uridine 5'-monophosphate ChEBI	+	assimilation
[Ref.: #66740]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66740]		catalase	-	1.11.1.6
[Ref.: #66740]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67771]	Sample type/isolated from	From Sansha Yongle Blue Hole of Paracel Islands in the South China Sea at a depth of 90 m
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Younle Blue Hole in the South China Sea at a depth of 90 m

[Ref.: #66740]	Geographic location (country and/or sea, region)	Yongle Blue Hole in the South China Sea
[Ref.: #66740]	Country	China
[Ref.: #66740]	Country ISO 3 Code	CHN
[Ref.: #66740]	Continent	Asia
[Ref.: #66740]	Geographic location	16.5250°/111.7680°
[Ref.: #66740]	Enrichment culture	sterile fluid enrichment medium
[Ref.: #66740]	Enrichment culture composition	per litre: 5 g peptone, 1 g yeast extract, 0.01 g FePO 4 , 0.3 g cysteine and 0.001 g resazurin
[Ref.: #66740]	Enrichment culture duration	1 month
[Ref.: #66740]	Enrichment culture temperature	16 ̊C
[Ref.: #66740]	Isolation/enrichment procedure	Strain JC075 was isolated from the Yongle Blue Hole in the South China Sea at water depth of 90 m in the water column. The seawater samples were quickly inoculated into a sterile fluid enrichment medium. The medium (per litre: 5 g peptone, 1 g yeast extract, 0.01 g FePO 4 , 0.3 g cysteine and 0.001 g resazurin) was prepared anaerobically and kept under an atmosphere of mixed gas (10 % H2 , 20 % CO2 and 70 % N2 ). Enrichment cultures were incubated at 16°C in 10 ml vials sealed with butyl-rubber stoppers. After 1 month, the bacterial liquid was coated on marine agar 2216 in an anaerobic packet of AneroPack-Anaero for bacterial purification.
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67771]	Marinifilum breve strain JC075, whole genome shotgun sequencing project	QFLI00000000	213	ENA	2184082 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67771]	Marinifilum breve JC075	GCA_003202155	contig	GenBank	2184082 tax ID
[Ref.: #66792]	Marinifilum breve JC075	2832567879	draft	JGI IMG/M	2184082 tax ID	*

[Ref.: #67770]

GC-content

35.8 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #66740]

Culture collection no.

KCTC 15646, MCCC 1K03477, JCM 32401, MCC 1K03477

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinifilum breve sp. nov., a marine bacterium isolated from the Yongle Blue Hole in the South China Sea and emended description of the genus Marinifilum.	Fu T, Jia C, Fu L, Zhou S, Yao P, Du R, Sun H, Yang Z, Shi X, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003027	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66740

Tianyu Fu, Chao Jia, Liang Fu, Shun Zhou, Peng Yao, Rui Du, Hao Sun, Zuosheng Yang, Xiaochong Shi, Xiao-Hua Zhang: Marinifilum breve sp. nov., a marine bacterium isolated from the Yongle Blue Hole in the South China Sea and emended description of the genus Marinifilum. IJSEM 68: 3540 - 3545 2018 ( DOI 10.1099/ijsem.0.003027 , PubMed 30230441 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Marinifilum breve in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF