Name: Nocardia fusca CIP 104513
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 136777

Type strain:

Species: Nocardia fusca

Culture col. no.: CIP 104513, IFO 14340, JCM 10427, BCRC 13734, NBRC 14340, DSM 100631, IMSNU 21193

Strain history: CIP <- 1995, IFO, "Nocardia fusca" <- IMAS

NCBI tax ID(s): 1223549 (strain), 941183 (species)

Section

Nocardia fusca CIP 104513 is an obligate aerobe, spore-forming, mesophilic prokaryote of the family Not assigned to family.

Gram-positive
spore-forming
mesophilic
obligate aerobe
16S sequence
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

[Ref.: #36347]	Phylum	Not assigned to order
[Ref.: #36347]	Class	Not assigned to order
[Ref.: #36347]	Order	Not assigned to order
[Ref.: #36347]	Family	Not assigned to family
[Ref.: #36347]	Genus	Nocardia
[Ref.: #36347]	Species	Nocardia fusca
[Ref.: #36347]	Type strain

Information on morphological and physiological properties Morphology

[Ref.: #36347]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #36347]

Culture medium

MEDIUM 3 - Columbia agar

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Culture medium growth

[Ref.: #36347]

Culture medium composition

expand / minimize

[Ref.: #36347]

Culture medium

CIP Medium 3

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Culture medium growth

[Ref.: #36347]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #36347]		growth	30 °C
[Ref.: #36347]		growth	30-37 °C
[Ref.: #36347]	no	growth	10 °C
[Ref.: #36347]	no	growth	45 °C
[Ref.: #67770]		growth	28 °C

[Ref.: #36347]	Temperature range	mesophilic
[Ref.: #36347]	Temperature range	psychrophilic
[Ref.: #36347]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #36347]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	yes Confidence in %95
[Ref.: #69480]	Ability of spore formation	no Confidence in %98.806

	Salt	Tested relation		Salt conc.
[Ref.: #36347]	NaCl		growth	0-6	%
[Ref.: #36347]	NaCl	no	growth	8	%
[Ref.: #36347]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #36347]	esculin ChEBI	+	hydrolysis
[Ref.: #36347]	hippurate ChEBI	-	hydrolysis
[Ref.: #36347]	nitrate ChEBI	+	reduction
[Ref.: #36347]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #36347]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #36347]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #36347]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #36347]	amylase	-
[Ref.: #36347]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #36347]	caseinase	-	3.4.21.50
[Ref.: #36347]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #36347]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #36347]	gelatinase	-
[Ref.: #36347]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #36347]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #36347]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #36347]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #36347]	tween esterase	-
[Ref.: #36347]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #36347]	691	not determinedn.d.	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #36347]	8005	-	+	+	+	-	+	-	-	+	-	+	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Beijing
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #36347]	Sample type/isolated from	Environment, Soil
[Ref.: #36347]	Country	China
[Ref.: #36347]	Country ISO 3 Code	CHN
[Ref.: #36347]	Continent	Asia

[Ref.: #36347]	Country	China
[Ref.: #36347]	Country ISO 3 Code	CHN
[Ref.: #36347]	Continent	Asia
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence AB647161 (>99% sequence identity) for Nocardia from Microbeatlas

Aquatic Samples	469
Soil Samples	8278
Animal Samples	943
Plant Samples	1687
Total Samples	11377

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #36347]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardia fusca gene for 16S rRNA, partial sequence	AB647161	1457	ENA	941183 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardia fusca NBRC 14340	GCA_001618425	contig	GenBank	1223549 tax ID
[Ref.: #66792]	Nocardia fusca NBRC 14340	1223549.4	wgs	PATRIC	1223549 tax ID	*
[Ref.: #66792]	Nocardia fusca NBRC 14340	2681812988	draft	JGI IMG/M	1223549 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nocardia fusca NBRC 14340 PATRIC: 1223549.4
[Ref.: #69481]	spore-forming	yes	95	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nocardia fusca NBRC 14340 PATRIC: 1223549.4
[Ref.: #69480]	motile	no	93.072	no
[Ref.: #69480]	gram-positive	yes	87.881	no
[Ref.: #69480]	anaerobic	no	99.036	no
[Ref.: #69480]	aerobic	yes	88.357	no
[Ref.: #69480]	halophile	no	88.02	no
[Ref.: #69480]	spore-forming	yes	74.295	no
[Ref.: #69480]	glucose-util	yes	89.768	no
[Ref.: #69480]	flagellated	no	98.05	no
[Ref.: #69480]	glucose-ferment	no	92.15	no
[Ref.: #69480]	thermophile	no	98.955	yes

Availability in culture collections External links

[Ref.: #36347]

Culture collection no.

CIP 104513, IFO 14340, JCM 10427, BCRC 13734, NBRC 14340, DSM 100631, IMSNU 21193

References
#36347 Collection of Institut Pasteur ; Curators of the CIP; CIP 104513

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Nocardia fusca NBRC 14340

Nocardia fusca NBRC 14340

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