Strain identifier
BacDive ID: 971
Type strain: 
Species: Bacillus subtilis
Strain Designation: natto 3335 UM8, pLS20, 3335 UM8
NCBI tax ID(s): 1423 (species)
version 8.1 (current version)
General
@ref: 1668
BacDive-ID: 971
DSM-Number: 4450
keywords: Bacteria, human pathogen
description: Bacillus subtilis natto 3335 UM8 is a human pathogen of the family Bacillaceae.
NCBI tax id
- NCBI tax id: 1423
- Matching level: species
doi: 10.13145/bacdive971.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus subtilis
- full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Vibrio subtilis
@ref: 1668
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus subtilis
full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872
strain designation: natto 3335 UM8, pLS20, 3335 UM8
type strain: no
Safety information
risk assessment
- @ref: 1668
- pathogenicity human: yes
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 1668
culture collection no.: DSM 4450, PBC pBC16
straininfo link
- @ref: 70644
- straininfo: 112710
literature
| Pubmed-ID | title | authors | journal | DOI | year | mesh | topic | topic2 |
|---|---|---|---|---|---|---|---|---|
| 25340403 | A complex genetic switch involving overlapping divergent promoters and DNA looping regulates expression of conjugation genes of a gram-positive plasmid. | Ramachandran G, Singh PK, Luque-Ortega JR, Yuste L, Alfonso C, Rojo F, Wu LJ, Meijer WJ | PLoS Genet | 10.1371/journal.pgen.1004733 | 2014 | Bacillus subtilis/genetics, *Conjugation, Genetic, Drug Resistance, Microbial/*genetics, Gene Expression Regulation, Bacterial, Plasmids/*genetics, Promoter Regions, Genetic, *Transcription, Genetic | ||
| 26112413 | Induction of Plasmid Conjugation in Bacillus subtilis Is Bistable and Driven by a Direct Interaction of a Rap/Phr Quorum-sensing System with a Master Repressor. | Rosch TC, Graumann PL | J Biol Chem | 10.1074/jbc.M115.664110 | 2015 | Bacillus subtilis/*physiology, *Conjugation, Genetic, Operon, *Plasmids, *Quorum Sensing, Repressor Proteins/*physiology | ||
| 28207825 | Discovery of a new family of relaxases in Firmicutes bacteria. | Ramachandran G, Miguel-Arribas A, Abia D, Singh PK, Crespo I, Gago-Cordoba C, Hao JA, Luque-Ortega JR, Alfonso C, Wu LJ, Boer DR, Meijer WJ | PLoS Genet | 10.1371/journal.pgen.1006586 | 2017 | Amino Acid Sequence, Bacillus subtilis/enzymology, Bacterial Proteins/*genetics, *Conjugation, Genetic, DNA, Single-Stranded/genetics, Drug Resistance, Bacterial/*genetics, Endodeoxyribonucleases/*genetics/isolation & purification, Firmicutes/*enzymology/genetics, Gastrointestinal Microbiome/genetics, Gene Transfer, Horizontal, Humans, Plasmids/genetics | Enzymology | Pathogenicity |
| 29163424 | The Bacillus subtilis Conjugative Plasmid pLS20 Encodes Two Ribbon-Helix-Helix Type Auxiliary Relaxosome Proteins That Are Essential for Conjugation. | Miguel-Arribas A, Hao JA, Luque-Ortega JR, Ramachandran G, Val-Calvo J, Gago-Cordoba C, Gonzalez-Alvarez D, Abia D, Alfonso C, Wu LJ, Meijer WJJ | Front Microbiol | 10.3389/fmicb.2017.02138 | 2017 | |||
| 29316866 | The first high frequency of recombination-like conjugal transfer from an integrated origin of transfer sequence in Bacillus subtilis 168. | Itaya M, Hasegawa M, Tomita M, Sato M | Biosci Biotechnol Biochem | 10.1080/09168451.2017.1422970 | 2018 | Bacillus subtilis/drug effects/*genetics, Conjugation, Genetic/*genetics, Drug Resistance, Microbial/genetics, *Gene Transfer Techniques, Genes, Bacterial/genetics, Plasmids/genetics, Recombination, Genetic/*genetics | Genetics | Pathogenicity |
| 29374463 | Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer. | Miyano M, Tanaka K, Ishikawa S, Takenaka S, Miguel-Arribas A, Meijer WJJ, Yoshida KI | Microb Cell Fact | 10.1186/s12934-017-0855-x | 2018 | Bacillus subtilis/*genetics, Chromosomes, Bacterial/*genetics, *Conjugation, Genetic, DNA, Bacterial/*genetics, Gene Transfer Techniques, Plasmids/*genetics | ||
| 30726909 | Stable and efficient delivery of DNA to Bacillus subtilis (natto) using pLS20 conjugational transfer plasmids. | Itaya M, Nagasaku M, Shimada T, Ohtani N, Shiwa Y, Yoshikawa H, Kaneko S, Tomita M, Sato M | FEMS Microbiol Lett | 10.1093/femsle/fnz032 | 2019 | Bacillus subtilis/*genetics, Conjugation, Genetic, DNA, Bacterial/*genetics, *Gene Transfer Techniques, Genetic Engineering/*methods, Mutation, Plasmids/*genetics | ||
| 31004491 | Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168: hub strains for giant DNA engineering. | Itaya M, Sato M, Watanabe S, Yoshikawa H, Tomita M, Sato R | J Biochem | 10.1093/jb/mvz031 | 2019 | Bacillus subtilis/*genetics, DNA, Bacterial/*genetics, *Genetic Engineering, Mutation | Biotechnology | |
| 31354647 | Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20. | Gago-Cordoba C, Val-Calvo J, Miguel-Arribas A, Serrano E, Singh PK, Abia D, Wu LJ, Meijer WJJ | Front Microbiol | 10.3389/fmicb.2019.01502 | 2019 | |||
| 32658272 | Inactivation of the dimeric RappLS20 anti-repressor of the conjugation operon is mediated by peptide-induced tetramerization. | Crespo I, Bernardo N, Miguel-Arribas A, Singh PK, Luque-Ortega JR, Alfonso C, Malfois M, Meijer WJJ, Boer DR | Nucleic Acids Res | 10.1093/nar/gkaa540 | 2020 | Bacillus subtilis, Bacterial Proteins/chemistry/genetics/*metabolism, Conjugation, Genetic/genetics, *Gene Expression Regulation, Bacterial, *Operon, Peptides/metabolism, Promoter Regions, Genetic, *Protein Multimerization, Tetratricopeptide Repeat, Trans-Activators/chemistry/genetics/*metabolism | Metabolism | Genetics |
| 33045732 | Reversible regulation of conjugation of Bacillus subtilis plasmid pLS20 by the quorum sensing peptide responsive anti-repressor RappLS20. | Singh PK, Serrano E, Ramachandran G, Miguel-Arribas A, Gago-Cordoba C, Val-Calvo J, Lopez-Perez A, Alfonso C, Wu LJ, Luque-Ortega JR, Meijer WJJ | Nucleic Acids Res | 10.1093/nar/gkaa797 | 2020 | Bacillus subtilis/*genetics/metabolism/physiology, Bacterial Proteins/*genetics/metabolism, *Conjugation, Genetic, *Gene Expression Regulation, Bacterial, Plasmids/genetics, Promoter Regions, Genetic, *Quorum Sensing | Metabolism | |
| 33727345 | A Conserved Class II Type Thioester Domain-Containing Adhesin Is Required for Efficient Conjugation in Bacillus subtilis. | Gago-Cordoba C, Val-Calvo J, Abia D, Diaz-Talavera A, Miguel-Arribas A, Aguilar Suarez R, van Dijl JM, Wu LJ, Meijer WJJ | mBio | 10.1128/mBio.00104-21 | 2021 | Adhesins, Bacterial/*chemistry/*genetics, Bacillus subtilis/*genetics/pathogenicity, Bacterial Proteins/*genetics/metabolism, Conjugation, Genetic/*genetics, DNA, Bacterial/genetics, Gene Transfer, Horizontal, Operon, Plasmids/genetics | Genetics | Metabolism |
| 33816561 | Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20. | Meijer WJJ, Boer DR, Ares S, Alfonso C, Rojo F, Luque-Ortega JR, Wu LJ | Front Mol Biosci | 10.3389/fmolb.2021.648468 | 2021 | |||
| 34576826 | Assessment of Bacillus subtilis Plasmid pLS20 Conjugation in the Absence of Quorum Sensing Repression. | Mori K, Verrone V, Amatsu R, Fukui K, Meijer WJJ, Ishikawa S, Wipat A, Yoshida KI | Microorganisms | 10.3390/microorganisms9091931 | 2021 | |||
| 34729475 | pLS20 is the archetype of a new family of conjugative plasmids harboured by Bacillus species. | Val-Calvo J, Miguel-Arribas A, Abia D, Wu LJ, Meijer WJJ | NAR Genom Bioinform | 10.1093/nargab/lqab096 | 2021 | |||
| 34946067 | Establishment Genes Present on pLS20 Family of Conjugative Plasmids Are Regulated in Two Different Ways. | Val-Calvo J, Miguel-Arribas A, Freire F, Abia D, Wu LJ, Meijer WJJ | Microorganisms | 10.3390/microorganisms9122465 | 2021 | |||
| 35198129 | Structural and biochemical characterization of the relaxosome auxiliary proteins encoded on the Bacillus subtilis plasmid pLS20. | Crespo I, Bernardo N, Cuppari A, Calisto BM, Val-Calvo J, Miguel-Arribas A, Meijer WJJ, Carpena X, Gil-Ortiz F, Malfois M, Boer DR | Comput Struct Biotechnol J | 10.1016/j.csbj.2021.12.041 | 2022 | |||
| 35260160 | A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation. | Mori K, Fukui K, Amatsu R, Ishikawa S, Verrone V, Wipat A, Meijer WJJ, Yoshida KI | Microb Cell Fact | 10.1186/s12934-022-01759-8 | 2022 | *Bacillus subtilis/genetics/metabolism, Chromosomes, *Geobacillus/genetics, Plasmids/genetics | Metabolism | |
| 35491108 | Bacillus subtilis 168 as a unique platform enabling synthesis and dissemination of genomes. | Itaya M | J Gen Appl Microbiol | 10.2323/jgam.2021.12.001 | 2022 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1668 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4450) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4450 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 70644 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID112710.1 | StrainInfo: A central database for resolving microbial strain identifiers |