Strain identifier
BacDive ID: 8598
Type strain: 
Species: Mesomycoplasma bovoculi
Strain Designation: M165/69
Strain history: <- J. K. Davis; M165/69 <- J. G. Tully <- ATCC <- NCTC <- R. H. Leach, MRL <- E. V. Langford
NCBI tax ID(s): 45362 (species)
version 8.1 (current version)
General
@ref: 15735
BacDive-ID: 8598
DSM-Number: 21484
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, animal pathogen
description: Mesomycoplasma bovoculi M165/69 is a mesophilic, Gram-negative animal pathogen that was isolated from eye of cattle with infectious bovine keratoconjunctivitis.
NCBI tax id
- NCBI tax id: 45362
- Matching level: species
strain history
- @ref: 15735
- history: <- J. K. Davis; M165/69 <- J. G. Tully <- ATCC <- NCTC <- R. H. Leach, MRL <- E. V. Langford
doi: 10.13145/bacdive8598.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/mycoplasmatota
- domain: Bacteria
- phylum: Mycoplasmatota
- class: Mollicutes
- order: Mycoplasmoidales
- family: Metamycoplasmataceae
- genus: Mesomycoplasma
- species: Mesomycoplasma bovoculi
- full scientific name: Mesomycoplasma bovoculi (Langford and Leach 1973) Gupta et al. 2019
synonyms
@ref synonym 20215 Mesomycoplasma bovoculi 20215 Mycoplasma bovoculi
@ref: 15735
domain: Bacteria
phylum: Tenericutes
class: Mollicutes
order: Mycoplasmatales
family: Metamycoplasmataceae
genus: Mesomycoplasma
species: Mesomycoplasma bovoculi
full scientific name: Mesomycoplasma bovoculi (Langford and Leach 1973) Gupta et al. 2019
strain designation: M165/69
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 98.803
Culture and growth conditions
culture medium
- @ref: 15735
- name: SP4-Z MEDIUM (DSMZ Medium 1076b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1076b
- composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
culture temp
- @ref: 15735
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.996 |
Isolation, sampling and environmental information
isolation
- @ref: 15735
- sample type: eye of cattle with infectious bovine keratoconjunctivitis
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Infection | #Disease | |
| #Host | #Mammals | #Bovinae (Cow, Cattle) |
| #Host Body-Site | #Organ | #Eye |
taxonmaps
- @ref: 69479
- File name: preview.99_7078.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15820;96_3320;97_4071;98_5204;99_7078&stattab=map
- Last taxonomy: Mycoplasma bovoculi subclade
- 16S sequence: U44768
- Sequence Identity:
- Total samples: 359
- soil counts: 26
- aquatic counts: 4
- animal counts: 326
- plant counts: 3
Safety information
risk assessment
- @ref: 15735
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Mycoplasma bovoculi strain ATCC 29104 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | AY785380 | 864 | ena | 743966 |
| 15735 | Mycoplasma bovoculi M165/69 16S ribosomal RNA (rrn) gene, partial sequence | U44768 | 1474 | ena | 743966 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Mycoplasma bovoculi M165/69 | 743966.3 | complete | patric | 743966 |
| 66792 | Mesomycoplasma bovoculi M165/69 | GCA_000524555 | complete | ncbi | 743966 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 93.065 | no |
| flagellated | no | 97.292 | no |
| gram-positive | yes | 52.226 | no |
| anaerobic | no | 97.972 | no |
| aerobic | no | 91.694 | no |
| halophile | yes | 50.667 | no |
| spore-forming | no | 98.698 | no |
| thermophile | no | 95.163 | no |
| glucose-util | yes | 87.168 | no |
| glucose-ferment | yes | 76.948 | no |
External links
@ref: 15735
culture collection no.: DSM 21484, ATCC 29104, NCTC 10141
straininfo link
- @ref: 77982
- straininfo: 44811
literature
| Pubmed-ID | title | authors | journal | year | mesh | topic | DOI |
|---|---|---|---|---|---|---|---|
| 2461129 | Attachment of Mycoplasma bovoculi to bovine conjunctival epithelium and lung fibroblasts. | Salih BA, Rosenbusch RF | Am J Vet Res | 1988 | Animals, Antigens, Bacterial/analysis, Antigens, Surface/analysis, *Bacterial Adhesion, Cattle, Cells, Cultured, Conjunctiva/*microbiology, Epithelium/microbiology, Epitopes/analysis, Fibroblasts/microbiology, Immunohistochemistry, Lung/cytology/*microbiology, Microscopy, Electron, Mycoplasma/immunology/*physiology/ultrastructure | ||
| 24558249 | Complete Genome Sequence of Mycoplasma bovoculi Strain M165/69T (ATCC 29104). | Calcutt MJ, Foecking MF | Genome Announc | 2014 | Genetics | 10.1128/genomeA.00115-14 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 15735 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21484) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21484 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 77982 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44811.1 | StrainInfo: A central database for resolving microbial strain identifiers |