Strain identifier
BacDive ID: 8591
Type strain: 
Species: Mycobacterium minnesotense
Strain Designation: DL49
Strain history: J. L. Dahl; Univ. of Minnesota Duluth, USA; DL49.
NCBI tax ID(s): 1118379 (species)
version 8.1 (current version)
General
@ref: 17964
BacDive-ID: 8591
DSM-Number: 45633
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive, rod-shaped
description: Mycobacterium minnesotense DL49 is a mesophilic, Gram-positive, rod-shaped bacterium that was isolated from sphagnum peat bog.
NCBI tax id
- NCBI tax id: 1118379
- Matching level: species
strain history
| @ref | history |
|---|---|
| 17964 | <- J. Dahl, University of Minnesota Duluth,USA; DL49 |
| 67770 | J. L. Dahl; Univ. of Minnesota Duluth, USA; DL49. |
doi: 10.13145/bacdive8591.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium minnesotense
- full scientific name: Mycobacterium minnesotense Hannigan et al. 2013
synonyms
- @ref: 20215
- synonym: Mycolicibacter minnesotensis
@ref: 17964
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium minnesotense
full scientific name: Mycobacterium minnesotense Hannigan et al. 2013 emend. Nouioui et al. 2018
strain designation: DL49
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell shape | motility | confidence |
|---|---|---|---|---|
| 30493 | positive | rod-shaped | no | |
| 69480 | no | 96.943 | ||
| 69480 | positive | 100 |
pigmentation
- @ref: 30493
- production: yes
Culture and growth conditions
culture medium
- @ref: 17964
- name: MIDDLEBROOK MEDIUM (DSMZ Medium 645)
- growth: yes
- link: https://mediadive.dsmz.de/medium/645
- composition: Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 17964 | positive | growth | 28 | mesophilic |
| 30493 | positive | growth | 28-34 | mesophilic |
| 30493 | positive | optimum | 30 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 30493 | no | |
| 69481 | no | 100 |
| 69480 | no | 99.963 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 30493 | NaCl | positive | growth | 5 % |
| 30493 | NaCl | positive | optimum | 5 % |
metabolite utilization
- @ref: 30493
- Chebi-ID: 53426
- metabolite: tween 80
- utilization activity: +
- kind of utilization tested: carbon source
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 30493 | arylsulfatase | + | 3.1.6.1 |
| 30493 | catalase | + | 1.11.1.6 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 17964 | sphagnum peat bog | Northern Minnesota | USA | USA | North America |
| 67770 | Sphagnum peat bog in northern Minnesota | USA | USA | North America |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Wetland (Swamp) |
| #Host | #Plants | #Peat moss |
Safety information
risk assessment
- @ref: 17964
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17964
- description: Mycobacterium minnesotense strain DL49 16S ribosomal RNA gene, partial sequence
- accession: JN546610
- length: 862
- database: ena
- NCBI tax ID: 1118379
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Mycolicibacter minnesotensis JCM 17932 | GCA_010731755 | complete | ncbi | 1118379 |
| 66792 | Mycobacterium minnesotense strain DSM 45633 | 1118379.3 | wgs | patric | 1118379 |
| 66792 | Mycolicibacter minnesotensis strain JCM 17932 | 1118379.12 | complete | patric | 1118379 |
| 66792 | Mycolicibacter minnesotensis DSM 45633 | 2865558272 | draft | img | 1118379 |
| 67770 | Mycolicibacter minnesotensis DSM 45633 | GCA_002086405 | contig | ncbi | 1118379 |
| 66792 | Mycolicibacter minnesotensis DSM 45633 | GCA_025821425 | scaffold | ncbi | 1118379 |
GC content
- @ref: 67770
- GC-content: 67.1
- method: genome sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 95.542 | yes |
| flagellated | no | 98.776 | yes |
| gram-positive | yes | 90.774 | yes |
| anaerobic | no | 99.153 | no |
| aerobic | yes | 91.157 | no |
| halophile | no | 92.955 | no |
| spore-forming | no | 89.8 | yes |
| glucose-util | yes | 57.015 | no |
| thermophile | no | 98.823 | yes |
| glucose-ferment | no | 92.612 | no |
External links
@ref: 17964
culture collection no.: DSM 45633, JCM 17932, NCCB 100399
straininfo link
- @ref: 77975
- straininfo: 400763
literature
- topic: Phylogeny
- Pubmed-ID: 22345139
- title: Mycobacterium minnesotense sp. nov., a photochromogenic bacterium isolated from sphagnum peat bogs.
- authors: Hannigan GD, Krivogorsky B, Fordice D, Welch JB, Dahl JL
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.037291-0
- year: 2012
- mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Genes, Bacterial, Minnesota, Molecular Sequence Data, Mycobacterium/*classification/genetics/isolation & purification, Mycolic Acids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Sphagnopsida/*microbiology, Wetlands
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 17964 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45633) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45633 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30493 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26826 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 77975 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID400763.1 | StrainInfo: A central database for resolving microbial strain identifiers |