Strain identifier

BacDive ID: 8489

Type strain: No

Species: Mycobacterium holsaticum

Strain Designation: 5050

Strain history: <- E. Richter, Research Center, Borstel; 5050

NCBI tax ID(s): 152142 (species)

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General

@ref: 11805

BacDive-ID: 8489

DSM-Number: 44479

keywords: Bacteria, aerobe, mesophilic, human pathogen

description: Mycobacterium holsaticum 5050 is an aerobe, mesophilic human pathogen that was isolated from human urine.

NCBI tax id

  • NCBI tax id: 152142
  • Matching level: species

strain history

  • @ref: 11805
  • history: <- E. Richter, Research Center, Borstel; 5050

doi: 10.13145/bacdive8489.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium holsaticum
  • full scientific name: Mycobacterium holsaticum Richter et al. 2002

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium holsaticum

@ref: 11805

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium holsaticum

full scientific name: Mycobacterium holsaticum Richter et al. 2002

strain designation: 5050

type strain: no

Culture and growth conditions

culture medium

  • @ref: 11805
  • name: MIDDLEBROOK MEDIUM (DSMZ Medium 645)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/645
  • composition: Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water

culture temp

@refgrowthtypetemperaturerange
11805positivegrowth37mesophilic
57134positivegrowth31mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 57134
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379catalase-1.11.1.6
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
57134++-+--+-++-----------

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
57134---+-+----+---------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic location
11805human urineGermanyDEUEurope
57134Human urineGermanyDEUEurope1997Borstel

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Urine

Safety information

risk assessment

  • @ref: 11805
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 11805
  • GC-content: 68.4

External links

@ref: 11805

culture collection no.: ATCC BAA 772, DSM 44479, CCUG 46267

straininfo link

  • @ref: 77883
  • straininfo: 265583

literature

  • topic: Phylogeny
  • Pubmed-ID: 12508858
  • title: Mycobacterium holsaticum sp. nov.
  • authors: Richter E, Niemann S, Gloeckner FO, Pfyffer GE, Rusch-Gerdes S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-6-1991
  • year: 2002
  • mesh: *Bacterial Proteins, Base Composition, Base Sequence, Chaperonin 60, Chaperonins/genetics, DNA, Bacterial/chemistry/genetics, DNA, Intergenic/genetics, DNA, Ribosomal/genetics, Genes, Bacterial, Humans, Lipids/analysis, Molecular Sequence Data, Mycobacterium/*classification/genetics/*isolation & purification/metabolism, Mycobacterium tuberculosis/classification/genetics, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11805Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44479)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44479
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
57134Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46267)https://www.ccug.se/strain?id=46267
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
77883Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265583.1StrainInfo: A central database for resolving microbial strain identifiers