Strain identifier

BacDive ID: 8450

Type strain: No

Species: Mycobacterium mucogenicum

Strain Designation: MO12, 77-590, MO 12

Strain history: CIP <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent. at Tyler, TX, USA: strain MO 12 <- V. Silcox, CDC: strain 77-590

NCBI tax ID(s): 1226753 (strain), 56689 (species)

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General

@ref: 11483

BacDive-ID: 8450

DSM-Number: 44124

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Mycobacterium mucogenicum MO12 is an obligate aerobe, mesophilic bacterium that was isolated from cause of peritonitis.

NCBI tax id

NCBI tax idMatching level
1226753strain
56689species

strain history

@refhistory
11483<- A. F. Yassin; <- ATCC; ATCC 49649 <- R. J. WallaceJr; MO12 (Mycobacterium chelonae-like organism) <- V. A. Silcox, CDC, Atlanta; CDC 77-590
121159CIP <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent. at Tyler, TX, USA: strain MO 12 <- V. Silcox, CDC: strain 77-590

doi: 10.13145/bacdive8450.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium mucogenicum
  • full scientific name: Mycobacterium mucogenicum Springer et al. 1995

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium mucogenicum

@ref: 11483

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium mucogenicum

full scientific name: Mycobacterium mucogenicum Springer et al. 1995

strain designation: MO12, 77-590, MO 12

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.755
69480100positive
121159norod-shaped

multimedia

  • @ref: 11483
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44124.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11483MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11483LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
36360MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121159CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
11483positivegrowth37mesophilic
36360positivegrowth30mesophilic
121159positivegrowth30mesophilic
121159nogrowth10psychrophilic
121159nogrowth22psychrophilic
121159nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121159
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.934

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
121159606565hippurate-hydrolysis
12115917632nitrate+reduction
12115916301nitrite-reduction
12115917632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 121159
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
121159oxidase+
121159beta-galactosidase+3.2.1.23
121159gelatinase-
121159amylase-
121159DNase-
121159caseinase-3.4.21.50
121159catalase+1.11.1.6
121159tween esterase-
121159gamma-glutamyltransferase-2.3.2.2
121159lecithinase-
121159lipase-
121159protease-
121159urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
11483---+--+-------------+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121159--++++----++----+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121159++----------------+--------------+---------------+---+-----++----------+++---+-++---+-+--++-+++-+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
11483cause of peritonitisSeattleUSAUSANorth America47.608-122.335
121159Human, Peritoneal fluidSeattle, WashingtonUnited States of AmericaUSANorth America1976

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host Body-Site#Other#Abdomen

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
114832Risk group (German classification)
1211591Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium mucogenicum strain ATCC 49649 16S ribosomal RNA gene, partial sequenceAF4805851435ena56689
20218Mycobacterium mucogenicum strain ATCC 49649 16S ribosomal RNA gene, partial sequenceAY4570731482ena56689
20218Mycobacterium Chelonae-like organism 16S rRNA geneX807731073ena1774

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium mucogenicum DSM 44124GCA_005670685completencbi1226753
66792Mycolicibacterium mucogenicum DSM 44124GCA_009192845contigncbi1226753
66792Mycolicibacterium DSM 441241226753.5wgspatric1226753
66792Mycolicibacterium DSM 441241226753.4wgspatric1226753

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.674no
motileno94.674no
flagellatedno98.421no
flagellatedno98.421no
gram-positiveyes88.019no
gram-positiveyes88.019no
anaerobicno99.078no
anaerobicno99.078no
aerobicyes83.924no
aerobicyes83.924no
halophileno93.013no
halophileno93.013no
spore-formingno87.518no
spore-formingno87.518no
glucose-utilyes84.047no
glucose-utilyes84.047no
thermophileno99.429yes
thermophileno99.429yes
glucose-fermentno90.627yes
glucose-fermentno90.627yes

External links

@ref: 11483

culture collection no.: DSM 44124, ATCC 49649, CDC 77-590, CIP 105225

straininfo link

  • @ref: 77848
  • straininfo: 46888

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7537060Phylogeny of the Mycobacterium chelonae-like organism based on partial sequencing of the 16S rRNA gene and proposal of Mycobacterium mucogenicum sp. nov.Springer B, Bottger EC, Kirschner P, Wallace RJ JrInt J Syst Bacteriol10.1099/00207713-45-2-2621995Base Sequence, DNA, Bacterial/*genetics, Genes, Bacterial, Humans, Molecular Sequence Data, Mycobacterium/*classification/genetics/physiology, Mycobacterium chelonae/classification/genetics, Phylogeny, RNA, Bacterial/*genetics, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, Sequence Homology, Nucleic Acid, Water MicrobiologyPathogenicity
Phylogeny15545441Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing.Adekambi T, Drancourt MInt J Syst Evol Microbiol10.1099/ijs.0.63094-02004Bacterial Proteins/*genetics, Chaperonin 60, Chaperonins/*genetics, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, DNA-Directed RNA Polymerases/*genetics, Genes, rRNA, Molecular Sequence Data, Mycobacterium/classification/*genetics, Mycobacterium chelonae/classification/genetics, Mycobacterium fortuitum/classification/genetics, Mycobacterium smegmatis/classification/genetics, Nontuberculous Mycobacteria/classification/*genetics, *Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/*genetics, Sequence Analysis, DNA, Superoxide Dismutase/*geneticsEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11483Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44124)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44124
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36360Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17221
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77848Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46888.1StrainInfo: A central database for resolving microbial strain identifiers
121159Curators of the CIPCollection of Institut Pasteur (CIP 105225)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105225