Strain identifier

BacDive ID: 8448

Type strain: Yes

Species: Mycobacterium austroafricanum

Strain Designation: E9789-SA 12441, SA12441, 9789, 14 150 0001

Strain history: CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 150 0001 <- ATCC <- M. Tsukamura: strain 9789 <- H.J. Nieuwoudt: strain SA12441

NCBI tax ID(s): 39687 (species)

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General

@ref: 11589

BacDive-ID: 8448

DSM-Number: 44191

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Mycobacterium austroafricanum E9789-SA 12441 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from water.

NCBI tax id

  • NCBI tax id: 39687
  • Matching level: species

strain history

@refhistory
11589<- ATCC <- M. Tsukamura, 9789 <- H.J. Nieuwoudt
67770M. Tsukamura 30005 (=E9789) <-- H. J. van der Meulen SA12441.
116650CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 150 0001 <- ATCC <- M. Tsukamura: strain 9789 <- H.J. Nieuwoudt: strain SA12441

doi: 10.13145/bacdive8448.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium austroafricanum
  • full scientific name: Mycobacterium austroafricanum Tsukamura et al. 1983

  • synonyms

    @refsynonym
    20215Mycobacterium vanbaalenii
    20215Mycolicibacterium austroafricanum
    20215Mycolicibacterium vanbaalenii

@ref: 11589

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium austroafricanum

full scientific name: Mycobacterium austroafricanum Tsukamura et al. 1983 emend. Nouioui et al. 2018

strain designation: E9789-SA 12441, SA12441, 9789, 14 150 0001

type strain: yes

Morphology

cell morphology

  • @ref: 116650
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
20015Daffodil yellow (1007)10-14 days5006
20015Daffodil yellow (1007)10-14 daysBUG
20015Daffodil yellow (1007)10-14 daysMB7H9

multimedia

  • @ref: 11589
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44191.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11589MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11589LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
20015BUGyes
200155006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
20015MB7H9yesName: Middlebrook 7H9 (MB7H9) Broth Base Composition: Middelbrook 7H9 Broth Base 4.7 g/l Glycerol 2.0 g/l Casaminoacids 11.0 g/l Yeast extract 5.5 g/l Tween80 (20%) 2.5 g/l OADC-Enrichment 100 ml Agar 15.0 g/l Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Maintenance and revitalization Organisms: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
39282MEDIUM 55 - for Mycobacteriumyes
116650CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
20015positiveoptimum37mesophilic
11589positivegrowth37mesophilic
39282positivegrowth37mesophilic
67770positivegrowth37mesophilic
116650positivegrowth25-37mesophilic
116650nogrowth10psychrophilic
116650nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116650
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116650hippurate-hydrolysis606565
116650nitrate+reduction17632
116650nitrite-reduction16301
116650nitrate-respiration17632
68379esculin-hydrolysis4853
68379D-glucose-fermentation17634
68379D-ribose-fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379maltose-fermentation17306
68379lactose-fermentation17716
68379sucrose-fermentation17992
68379glycogen-fermentation28087

metabolite production

  • @ref: 116650
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379catalase+1.11.1.6
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
116650oxidase-
116650beta-galactosidase-3.2.1.23
116650gelatinase-
116650amylase-
116650DNase-
116650caseinase-3.4.21.50
116650catalase+1.11.1.6
116650tween esterase-
116650gamma-glutamyltransferase-2.3.2.2
116650lecithinase-
116650lipase-
116650urease+3.5.1.5
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
20015-+++--+--++--------
53794++++--+-------------+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20015+++-+----+----+----
53794+-+-+----++---------
116650-++-+-+--++---+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116650-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116650-+-----+-------------+----------++--------+------+----------+--------------+--+++---+---+-------+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
11589waterSouth AfricaZAFAfrica
53794Water
67770WaterSouth AfricaZAFAfrica
116650Environment, WaterSouth AfricaZAFAfrica

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115891Risk group (German classification)
200151German classification
1166501Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.austroafricanum 16S rRNA geneX931821462ena39687
20218Mycobacterium austroafricanum strain CIP 105395 16S ribosomal RNA gene, partial sequenceAF547897542ena39687

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium austroafricanum strain DSM 4419139687.3wgspatric39687
66792Mycobacterium austroafricanum strain DSM 4419139687.4wgspatric39687
66792Mycolicibacterium austroafricanum DSM 441912648501395draftimg39687
67770Mycolicibacterium austroafricanum DSM 44191GCA_000612725scaffoldncbi39687

GC content

  • @ref: 67770
  • GC-content: 67.7
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes87.254no
anaerobicno98.976no
halophileno91.813no
spore-formingno83.459no
glucose-utilyes86.017no
aerobicyes86.607no
motileno91.946no
flagellatedno97.736no
thermophileno99.389yes
glucose-fermentno91.012yes

External links

@ref: 11589

culture collection no.: DSM 44191, ATCC 33464, JCM 6369, CCUG 37667, CIP 105395, HAMBI 2271, KCTC 9504

straininfo link

  • @ref: 77846
  • straininfo: 40510

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12508859Classification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, Mycobacterium sp. strain PYR-1, as Mycobacterium vanbaalenii sp. nov.Khan AA, Kim SJ, Paine DD, Cerniglia CEInt J Syst Evol Microbiol10.1099/00207713-52-6-19972002Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Environmental Pollutants/metabolism, Fatty Acids/analysis, Geologic Sediments/microbiology, Mycobacterium/*classification/genetics/isolation & purification/*metabolism, Mycolic Acids/analysis, Petroleum/metabolism, Phenotype, Phylogeny, Polycyclic Aromatic Hydrocarbons/*metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityMetabolism
Metabolism12534814Degradation of straight-chain aliphatic and high-molecular-weight polycyclic aromatic hydrocarbons by a strain of Mycobacterium austroafricanum.Bogan BW, Lahner LM, Sullivan WR, Paterek JRJ Appl Microbiol10.1046/j.1365-2672.2003.01824.x2003Alkanes/metabolism, Benzopyrenes/metabolism, Culture Media, Fluorenes/metabolism, Fluorine/metabolism, Hydrophobic and Hydrophilic Interactions, Molecular Weight, Mycobacterium/growth & development/*metabolism, Phenanthrenes/metabolism, Polycyclic Aromatic Hydrocarbons/*metabolism, Pyrenes/metabolismCultivation
Genetics24744336Draft Genome Sequence of Mycobacterium austroafricanum DSM 44191.Croce O, Robert C, Raoult D, Drancourt MGenome Announc10.1128/genomeA.00317-142014
Phylogeny34878372Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species.Cheng Y, Lei W, Wang X, Tian Z, Liu H, Yang J, Lu S, Lai XH, Pu J, Huang Y, Zhang S, Yang C, Lian X, Bai Y, Wan K, Wang S, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0050192021Animals, Antelopes/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Feces/microbiology, Mycobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TibetTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11589Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44191)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44191
20015Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44191.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39282Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17409
53794Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37667)https://www.ccug.se/strain?id=37667
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
77846Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40510.1StrainInfo: A central database for resolving microbial strain identifiers
116650Curators of the CIPCollection of Institut Pasteur (CIP 105395)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105395